Evaluation on Anticancer Effect Against HL-60 Cells and Toxicity in vitro and in vivo of the Phenethyl Acetate Isolated from a Marine Bacterium, Streptomyces griseus
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We previously identified Streptomyces griseus as an anti-cancer agent (Kim et al., 2014). In this study, we isolated compounds from S. griseus and evaluated their anticancer effect and toxicity in vitro and in vivo. Preparative centrifugal partition chromatography (CPC) was used to obtain three compounds, cyclo(L-[4-hydroxyprolinyl]- L –leucine], cyclo(L-Phe-trans-4-hydroxy-L-Pro) and phenethyl acetate (PA). We chose PA, which had the highest anticancer activity, as a target compound for further experiments. PA induced the formation of apoptotic bodies, DNA fragmentation, DNA accumulation in G0/G1 phase, and reactive oxygen species (ROS) formation. Furthermore, PA treatment increased Bax/Bcl-xL expression, activated caspase-3, and cleaved poly-ADP-ribose polymerase (PARP) in HL-60 cells. Simultaneous evaluation in vitro and in vivo, revealed that PA exhibited no toxicity in Vero cells and zebrafish embryos. We revealed, for the first time, that PA generates ROS, and that this ROS accumulation induced the Bcl signaling pathway.
Abstract
We previously identified Streptomyces griseus as an anti-cancer agent (Kim et al., 2014). In this study, we isolated compounds from S. griseus and evaluated their anticancer effect and toxicity in vitro and in vivo. Preparative centrifugal partition chromatography (CPC) was used to obtain three compounds, cyclo(L-[4-hydroxyprolinyl]- L –leucine], cyclo(L-Phe-trans-4-hydroxy-L-Pro) and phenethyl acetate (PA). We chose PA, which had the highest anticancer activity, as a target compound for further experiments. PA induced the formation of apoptotic bodies, DNA fragmentation, DNA accumulation in G0/G1 phase, and reactive oxygen species (ROS) formation. Furthermore, PA treatment increased Bax/Bcl-xL expression, activated caspase-3, and cleaved poly-ADP-ribose polymerase (PARP) in HL-60 cells. Simultaneous evaluation in vitro and in vivo, revealed that PA exhibited no toxicity in Vero cells and zebrafish embryos. We revealed, for the first time, that PA generates ROS, and that this ROS accumulation induced the Bcl signaling pathway.
참고문헌 (44)
- Arctander S, "Perfume and Flavor Chemicals (Aroma Chemicals), 2"
- Bewley CA, "Lithistid sponges: Star performers or hosts to the stars?", Angewandte Chemie International Edition, pp.2162~2178 (1998)
- Bringmann G, "Evariquinone, isoemericellin, and stromemycin from a sponge derived strain of the fungus Emericella variecolor", Phytochemistry, 63, pp.437~443 (2003)
- Bringmann G, "The first sorbicillinoid alkaloids, the antileukemic sorbicillactones A and B, from a sponge-derived Penicillium chrysogenum strain", Tetrahedron, 61, pp.7252~7265 (2005)
- Cory S, "The Bcl2 family: regulators of the cellular life-or-death switch", Nat. Rev. Cancer, 2, pp.647~656 (2002)
- Coyle JT, "Oxidative stress, glutamate, and neurodegenerative disorders", Science, 262, pp.689~695 (1993)
- Demain AL, "Microbial drug discovery : 80 years of progress", J Antibiot, 62, pp.5~16 (2009)
- Faulkner DJ, "Drugs from the Sea", pp.107~119 (2000), Karger
- Fernandes-Alnemri T, "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein CED-3 and mammalian interleukin-1beta-converting enzyme", J Biol Chem, 269, pp.30761~30764 (1994)
- Garcia-Ruiz C, "Direct effect of ceramide on the mitochondrial electron transport chain leads to generation of reactive oxygen species", J Biol Chem, 272, pp.11369~11377 (1997)
- Han Z, "Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos", larvae exposed to tris(2-butoxyethyl)phosphate, 2014, pp.150, 175~181
- Haygood MG, "Small-subunit rRNA genes and in-situ hybridization with oligonucleotides specific for bacterial symbionts in larvae of the bryozoan Bugula neritina and proposal of"Candidatus Endobugula sertula"", Appl Environ Microbiol, 63, pp.4612~4616 (1997)
- Hentschel U, "Molecular evidence for a uniform microbial community in sponges from different oceans", Appl Environ Microb, 68, pp.4431~4440 (2002)
- Hseu YC, "Antioxidant activities of Toona Sinensis leaves extracts using different antioxidant models", Food Chem Toxicol, 46, pp.105~114 (2008)
- Hsieh FY, "Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish", Devel Biol, 375, pp.1~12 (2013)
- Jinap S, "Effect of roasting time and temperature on volatile component profiles during nib roasting of cocoa beans(Theobroma cacao)", J Sci Food Agricult, 77, pp.441~448 (1998)
- Kang MH, "Bcl-2 inhibitor : targeting mitochondrial apoptotic pathways in cancer therapy", Clin Cancer Res, 15, pp.1126~1132 (2009)
- Kang SM, "Neuroprotective effects of phlorotannins isolated from a brown alga, Ecklonia cava, against H2O2-induced oxidative stress in murine hippocampal HT22 cells", Environ Toxicol Pharmacol, 34, pp.96~105 (2012)
- Kim CY, "A preparative isolation and purification of arctigenin and matairesinol from Forsythia koreana by centrifugal partition chromatography", J Sep Sci, 29, pp.656~659 (2006)
- 김현수, "Evaluation of the Biological Activities of Marine Bacteria Collected from Jeju Island, Korea, and Isolation of Active Compounds from their Secondary Metabolites", Fisheries and Aquatic Sciences, 17(2), pp.215~222 (2014), 한국수산과학회
- Konopleva M, "Expression and function of leptin receptor isoforms in myeloid leukemia and myelodysplastic syndromes: proliferative and antiapoptotic activities", Blood, 93, pp.1668~1676 (1999)
- Lee JH, "Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step", Food Chem, 158, pp.433~437 (2014)
- Lee JH, "Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity", Food Chem Toxicol, 62, pp.54~60 (2013)
- Li GX, "Differential involvement of reactive oxygen species in apoptosis induced by two classes of selenium compounds in human prostate cancer cells", Intern J Cancer, 120, pp.2034~2043 (2007)
- Rui Liu, "Potent in Vitro Anticancer Activity of Metacycloprodigiosin and Undecylprodigiosin from a Sponge-Derived Actinomycete Saccharopolyspora sp. nov.", Archives of Pharmacal Research, 28(12), pp.1341~1344 (2005), 대한약학회
- MarinL, "A marine literature database produced and maintained by the Department of Chemistry", pp.2008
- Oliver FJ, "Importance of poly(ADP-ribose)polymerase and its cleavage in apoptosis lesson from an uncleavable mutant", J Biol Chem, 273, pp.33533~33539 (1998)
- Pelicano H, "ROS stress in cancer cells and therapeutic implications", Drug Resistance Updates, 7, pp.97~110 (2004)
- Pimentel-Elardo SM, "Anti-parasitic compounds from Streptomyces sp. strains isolated from Mediterranean sponges", Mar Drug, 8, pp.373~380 (2010)
- Proksch P, "Drugs from the seas : Current status and microbiological implications", Appl Microbiol Biotechnol, 59, pp.125~134 (2002)
- Rumyantsev VV, "Ustoichivost’i stabilizatsiya dvizheniya po otnosheniyu k chasti peremennykh" (1987), Nauka
- Schumacker PT, "Reactive oxygen species in cancer cells : live by the sword, die by the sword", Cancer Cell, 10, pp.175~176 (2006)
- Soles RM, "Ester Concentration Differences in Wine Fermented by Various Species and Strains of Yeasts", Am J Enol Vitic, 33, pp.94~98 (1982)
- Szatrowski TP, "Production of large amounts of hydrogen peroxide by human tumor cells", Cancer Res, 51, pp.794~798 (1991)
- Thoms C, "Biotechnological potential of marine sponges and their associated bacteria as producers of new pharmaceuticals(Part II)", J Intern Biotechnol Law, 2, pp.257~264 (2005)
- Thoms C, "Biotechnological potential of marine sponges and their associated bacteria as producers of new pharmaceuticals(Part I)", J Intern Biotechnol Law, 2, pp.217~220 (2005)
- Trachootham D, "Selective killing of ncogenically transformed cells through a ROS mediated mechanism by bphenylethyl isothiocyanate", Cancer Cell, 10, pp.241~252 (2006)
- Vacelet J, "Étude en microscopie électronique de l’association entre bactéries et spongiaires du genre Verongia(Dictyoceratida)", J Microscopy Biol Cell, 23, pp.271~288 (1975)
- Valko M, "Free radical, metals and antioxidants in oxidative stress-induce cancer", Chem Biol Intern, 160, pp.1~40 (2006)
- Wen J, "Oxidative stress-mediated apoptosis : the anticancer effect of the sesquiterpene lactone parthenolide", J Biolog Chem, 277, pp.38954~38964 (2002)
- Xu Y, "Corosolic acid induces apoptosis through mitochondrial pathway and caspases activation in human cervix adenocarcinoma HeLa cells", Cancer Lett, 284, pp.229~237 (2009)
- Yan Y, "Emodinazide methyl anthraquinone derivative trigger mitochondrial-dependent cell apoptosis involving in caspase-8-midiated Bid cleavage", Mol Cancer Ther, 7, pp.1688~1697 (2008)
- Zhang Z, "Role of reactive oxygen species and MAPKs in vanadate-induced G2", M phase arrest, 2003, pp.34, 1333~1342
- Zheng L, "Antimicrobial screening and active compound isolation from marine bacterium NJ6-3-1 associated with the sponge Hymeniacidon perleve", World J Microbiol Biotechnol, 21, pp.201~206 (2005)
