Anti-Inflammatory Effect of Cynanchone a in Lipopolysaccharide-Induced RAW 264.7 Cells
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It has been reported that Cynanchum wilfordii exhibits anti-oxidant, and anti-cancer activities. However, little is known about biological activity of cynanchone A, an acetophenone isolated from C. wilfordii. In the present study, we investigated the anti-inflammatory effect and the underlying molecular mechanisms of cynanchone A in lipopolysaccharide(LPS)-induced RAW 264.7 cells. During the LPS-induced inflammatory process in RAW 264.7 macrophages, nitric oxide(NO) and prostaglandin E2(PGE2) productions increased. Cynanchone A significantly decreased the protein levels of NO and PGE2 in LPS-treated RAW 264.7 macrophages. Moreover, cynanchone A significantly reduced the elevated the expression levels of inducible nitric oxide(iNOS), cyclooxygenase-2(COX-2) and tumor necrosis factor-a(TNF-a) in LPS-stimulated RAW264.7 cells. Consequently, cynanchone A exerted an anti-inflammatory action through inhibiting nuclear factor-kappa B(NF-κB) activation. Taken together, our results show that the anti-inflammatory properties of cyanchone A are involved in the down-regulating pro-inflammatory cytokines and regulating NF-kB signal pathway in LPS-stimulated RAW264.7 cells.
Abstract
It has been reported that Cynanchum wilfordii exhibits anti-oxidant, and anti-cancer activities. However, little is known about biological activity of cynanchone A, an acetophenone isolated from C. wilfordii. In the present study, we investigated the anti-inflammatory effect and the underlying molecular mechanisms of cynanchone A in lipopolysaccharide(LPS)-induced RAW 264.7 cells. During the LPS-induced inflammatory process in RAW 264.7 macrophages, nitric oxide(NO) and prostaglandin E2(PGE2) productions increased. Cynanchone A significantly decreased the protein levels of NO and PGE2 in LPS-treated RAW 264.7 macrophages. Moreover, cynanchone A significantly reduced the elevated the expression levels of inducible nitric oxide(iNOS), cyclooxygenase-2(COX-2) and tumor necrosis factor-a(TNF-a) in LPS-stimulated RAW264.7 cells. Consequently, cynanchone A exerted an anti-inflammatory action through inhibiting nuclear factor-kappa B(NF-κB) activation. Taken together, our results show that the anti-inflammatory properties of cyanchone A are involved in the down-regulating pro-inflammatory cytokines and regulating NF-kB signal pathway in LPS-stimulated RAW264.7 cells.
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