Investigation of optimum pantothenic acid level in the feeds for post-larvae fleshy prawn, Penaeus chinensis
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The present study assessed the dietary requirement of pantothenic acid (PA) for fleshy prawn (Penaeus chinensis) post-larvae. Five experimental diets were formulated by supplementing different levels of PA as 0, 100, 200, 300 and 400 mg kg–1 diet (designated as P0, P100, P200, P300 and P400, respectively). Triplicate groups of 50 prawns (initial mean body weight: 3 mg) per treatment were fed for 40 days. Prawns fed with P100 diet showed significantly higher (p < 0.05) growth and feed efficiency performance than P0 group. Survival rates in all PA supplemented groups were significantly higher (p < 0.05) than P0 group. However, treatments containing PA > 200 mg kg–1 demonstrated a negative trend with increasing PA levels. Upsurge of hepatopancreatic PA level was observed with increasing dietary PA level, just as P100 and P200 groups contained significantly higher (p < 0.05) hepatopancreatic PA levels than P0 and P400 groups. Meanwhile, prawns fed P200 diet showed significantly higher (p < 0.05) acyl-coenzyme A binding protein gene expression level than P0 group. Consistent with previous fatty acid binding protein gene expression level in P100 and P200 groups were significantly higher (p < 0.05) than that of P0 group. The gene expression of target of rapamycin showed no significant difference (p > 0.05) among the groups. Blasenzellen cell counts per tubule in prawn hepatopancreas were significantly higher (p < 0.05) in P100 group compared to P0 group. Similarly, compared to P0 group, fibrillar cell and resorptive cell count per tubule was significantly higher (p < 0.05) in P100 and P200 groups. When analyzing amino acid profiles, cysteine level in all the PA supplemented groups were significantly lower (p < 0.05) than that of P0 group. Histidine, serine and tryptophan levels in P200, P300 and P400 groups were significantly lower (p < 0.05) than that in P0 group. Isoleucine, methionine and threonine levels in P300 group was significantly higher (p < 0.05) than that in P0 group. Valine level in P400 group was significantly lower (p < 0.05) than that in P0 group. By the broken-line regression, optimum dietary PA requirements for fleshy prawn post-larvae were 93–123 mg kg–1 based on specific growth rate and whole-body PA level.
Abstract
The present study assessed the dietary requirement of pantothenic acid (PA) for fleshy prawn (Penaeus chinensis) post-larvae. Five experimental diets were formulated by supplementing different levels of PA as 0, 100, 200, 300 and 400 mg kg–1 diet (designated as P0, P100, P200, P300 and P400, respectively). Triplicate groups of 50 prawns (initial mean body weight: 3 mg) per treatment were fed for 40 days. Prawns fed with P100 diet showed significantly higher (p < 0.05) growth and feed efficiency performance than P0 group. Survival rates in all PA supplemented groups were significantly higher (p < 0.05) than P0 group. However, treatments containing PA > 200 mg kg–1 demonstrated a negative trend with increasing PA levels. Upsurge of hepatopancreatic PA level was observed with increasing dietary PA level, just as P100 and P200 groups contained significantly higher (p < 0.05) hepatopancreatic PA levels than P0 and P400 groups. Meanwhile, prawns fed P200 diet showed significantly higher (p < 0.05) acyl-coenzyme A binding protein gene expression level than P0 group. Consistent with previous fatty acid binding protein gene expression level in P100 and P200 groups were significantly higher (p < 0.05) than that of P0 group. The gene expression of target of rapamycin showed no significant difference (p > 0.05) among the groups. Blasenzellen cell counts per tubule in prawn hepatopancreas were significantly higher (p < 0.05) in P100 group compared to P0 group. Similarly, compared to P0 group, fibrillar cell and resorptive cell count per tubule was significantly higher (p < 0.05) in P100 and P200 groups. When analyzing amino acid profiles, cysteine level in all the PA supplemented groups were significantly lower (p < 0.05) than that of P0 group. Histidine, serine and tryptophan levels in P200, P300 and P400 groups were significantly lower (p < 0.05) than that in P0 group. Isoleucine, methionine and threonine levels in P300 group was significantly higher (p < 0.05) than that in P0 group. Valine level in P400 group was significantly lower (p < 0.05) than that in P0 group. By the broken-line regression, optimum dietary PA requirements for fleshy prawn post-larvae were 93–123 mg kg–1 based on specific growth rate and whole-body PA level.