Adenophora remotiflora protects human skin keratinocytes against UVB-induced photo-damage by regulating antioxidative activity and MMP-1 expression
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BACKGROUND/OBJECTIVES: Chronic ultraviolet (UV) exposure-induced reactive oxygen species (ROS) are commonly involved in the pathogenesis of skin damage by activating the metalloproteinases (MMP) that break down type I collagen. Adenophora remotiflora (AR) is a perennial wild plant that inhabits Korea, China, and Japan. The present study investigated the protective effects of AR against UVB-induced photo-damage in keratinocytes. MATERIALS/METHODS: An in vitro cell-free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitric oxide (NO). The effect of AR on ROS formation, antioxidant enzymes, elastase, MMP-1 level, and mRNA expression of MMP-1 were determined in UVB-irradiated human keratinocyte HaCaT cells. RESULTS: AR demonstrated strong DPPH free radical and NO scavenging activity in a cell-free system exhibiting IC50 values of 1.88 mg/mL and 6.77 mg/mL, respectively. AR pretreatment dose-dependently attenuated the production of UVB-induced intracellular ROS, and antioxidant enzymes (catalase and superoxide dismutase) were enhanced in HaCaT cells. Furthermore, pretreatment of AR prevented UVB-induced elastase and collagen degradation by inhibiting the MMP-1 protein level and mRNA expression. Accordingly, AR treatment elevated collagen content in UVB-irradiated HaCaT cells. CONCLUSION: The present study provides the first evidence of AR inhibiting UVB-induced ROS production and induction of MMP-1 as a result of augmentation of antioxidative activity in HaCaT human keratinocytes. These results suggest that AR might act as an effective inhibitor of UVB-modulated signaling pathways and might serve as a photo-protective agent.
Abstract
BACKGROUND/OBJECTIVES: Chronic ultraviolet (UV) exposure-induced reactive oxygen species (ROS) are commonly involved in the pathogenesis of skin damage by activating the metalloproteinases (MMP) that break down type I collagen. Adenophora remotiflora (AR) is a perennial wild plant that inhabits Korea, China, and Japan. The present study investigated the protective effects of AR against UVB-induced photo-damage in keratinocytes. MATERIALS/METHODS: An in vitro cell-free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitric oxide (NO). The effect of AR on ROS formation, antioxidant enzymes, elastase, MMP-1 level, and mRNA expression of MMP-1 were determined in UVB-irradiated human keratinocyte HaCaT cells. RESULTS: AR demonstrated strong DPPH free radical and NO scavenging activity in a cell-free system exhibiting IC50 values of 1.88 mg/mL and 6.77 mg/mL, respectively. AR pretreatment dose-dependently attenuated the production of UVB-induced intracellular ROS, and antioxidant enzymes (catalase and superoxide dismutase) were enhanced in HaCaT cells. Furthermore, pretreatment of AR prevented UVB-induced elastase and collagen degradation by inhibiting the MMP-1 protein level and mRNA expression. Accordingly, AR treatment elevated collagen content in UVB-irradiated HaCaT cells. CONCLUSION: The present study provides the first evidence of AR inhibiting UVB-induced ROS production and induction of MMP-1 as a result of augmentation of antioxidative activity in HaCaT human keratinocytes. These results suggest that AR might act as an effective inhibitor of UVB-modulated signaling pathways and might serve as a photo-protective agent.
참고문헌 (32)
- Pillai S, "Ultraviolet radiation and skin aging:roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review", Int J Cosmet Sci, 27, pp.17~34 (2005)
- Svobodová A, "Natural phenolics in the prevention of UV-induced skin damage. A review", Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 147, pp.137~145 (2003)
- de Gruijl FR., "Photocarcinogenesis: UVA vs UVB", Methods Enzymol, 319, pp.359~366 (2000)
- Podhaisky HP, "UV light and oxidative damage of the skin", Pharmazie, 57, pp.30~33 (2002)
- Sander CS, "Photoaging is associated with protein oxidation in human skin in vivo", J Invest Dermatol, 118, pp.618~625 (2002)
- Jang J, "Photo-oxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes", Environ Toxicol Pharmacol, 34, pp.926~934 (2012)
- Meyer W, "Elastic fibre arrangement in the skin of the pig", Arch Dermatol Res, 270, pp.391~401 (1981)
- Wiedow O, "Elafin:an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence", J Biol Chem, 265, pp.14791~14795 (1990)
- Nichols JA, "Skin photoprotection by natural polyphenols:anti-inflammatory, antioxidant and DNA repair mechanisms", Arch Dermatol Res, 302, pp.71~83 (2010)
- Kim TC., "Korean Resources Plants, IV", pp.183~189 (1998), Seoul National University Press
- 김애정, "The antioxidant and chemopreventive potentialities of Mosidae (Adenophora remotiflora) leaves", Nutrition Research and Practice, 4(1), pp.30~35 (2010), 한국영양학회
- 김성향, "모시대(Adenophora remotiflora) 추출물의 휘발성 성분 및 항산화 활성", 한국식품과학회지, 39(2), pp.109~113 (2007), 한국식품과학회
- Huang D, "The chemistry behind antioxidant capacity assays", J Agric Food Chem, 53, pp.1841~1856 (2005)
- Chan WH, "Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells", J Cell Biochem, 90, pp.327~338 (2003)
- Kraunsoe JA, "Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor", Biochemistry, 35, pp.9090~9096 (1996)
- Pfaffl MW., "A new mathematical model for relative quantification in real-time RT-PCR", Nucleic Acids Res, 29, pp.e45~ (2001)
- Wawszczyk J, "In vitro evaluation of antiproliferative and cytotoxic properties of pterostilbene against human colon cancer cells", Acta Pol Pharm, 71, pp.1051~1055 (2014)
- Russo PA, "Inhibition of nitric oxide and reactive oxygen species production improves the ability of a sunscreen to protect from sunburn, immunosuppression and photocarcinogenesis", Br J Dermatol, 155, pp.408~415 (2006)
- Deliconstantinos G, "Nitric oxide and peroxynitrite released by ultraviolet B-irradiated human endothelial cells are possibly involved in skin erythema and inflammation", Exp Physiol, 81, pp.1021~1033 (1996)
- Afaq F, "Botanical antioxidants in the prevention of photocarcinogenesis and photoaging", Exp Dermatol, 15, pp.678~684 (2006)
- Sawa T, "Alkylperoxyl radical-scavenging activity of various flavonoids and other phenolic compounds: implications for the anti-tumor-promoter effect of vegetables", J Agric Food Chem, 47, pp.397~402 (1999)
- Gülçin I, "In vitro antioxidant properties of morphine", Pharmacol Res, 49, pp.59~66 (2004)
- Shin MH, "H2O2 accumulation by catalase reduction changes MAP kinase signaling in aged human skin in vivo", J Invest Dermatol, 125, pp.221~229 (2005)
- Pluemsamran T, "Caffeic acid and ferulic acid inhibit UVA-induced matrix metalloproteinase-1 through regulation of antioxidant defense system in keratinocyte HaCaT cells", Photochem Photobiol, 88, pp.961~968 (2012)
- Zaid MA, "Inhibition of UVB-mediated oxidative stress and markers of photoaging in immortalized HaCaT keratinocytes by pomegranate polyphenol extract POMx", Photochem Photobiol, 83, pp.882~888 (2007)
- Graf BL, "Compounds leached from quinoa seeds inhibit matrix metalloproteinase activity and intracellular reactive oxygen species", Int J Cosmet Sci, 37, pp.212~221 (2015)
- Chiang HM, "Coffea arabica extract and its constituents prevent photoaging by suppressing MMPs expression and MAP kinase pathway", Food Chem Toxicol, 49, pp.309~318 (2011)
- Bae JY, "(-)Epigallocatechin gallate hampers collagen destruction and collagenase activation in ultraviolet-B-irradiated human dermal fibroblasts: involvement of mitogen-activated protein kinase", Food Chem Toxicol, 46, pp.1298~1307 (2008)
- Reeve VE, "Protection against photoaging in the hairless mouse by the isoflavone equol", Photochem Photobiol, 81, pp.1548~1553 (2005)
- Sun Z, "Salvianolic acid B protects normal human dermal fibroblasts against ultraviolet B irradiation-induced photoaging through mitogen-activated protein kinase and activator protein-1 pathways", Photochem Photobiol, 91, pp.879~886 (2015)
- Rittié L, "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1, pp.705~720 (2002)
- Uitto J., "The role of elastin and collagen in cutaneous aging: intrinsic aging versus photoexposure", J Drugs Dermatol, 7, pp.s12~s16 (2008)
