Identification of SNPs Affecting Porcine Carcass Weight with the 60K SNP Chip
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Carcass weight (CW) is one of the most important economic traits in pigs, directly affecting the income of farmers. In this study, a genome wide association study was performed to detect significant single nucleotide polymorphisms (SNPs) affecting CW in pigs derived from a F2 intercross between Landrace and Korean native pig (KNP). Using high-density porcine SNP chips,highly significant SNPs were identified on SSC12. Two candidate genes, LOC100523510 and LOC100621652, were subsequently selected within this region and further investigated. Within these candidate genes, five SNPs were identified and genotyped using the VeraCode GoldenGate assay. The results revealed that one SNP in the LOC100621652 gene and four SNPs in the LOC100523510 gene are highly associated with CW. These SNP markers can thus have significant applications for improving CW in KNP. However, the functions of these candidate genes are not fully understood and require further study.
Abstract
Carcass weight (CW) is one of the most important economic traits in pigs, directly affecting the income of farmers. In this study, a genome wide association study was performed to detect significant single nucleotide polymorphisms (SNPs) affecting CW in pigs derived from a F2 intercross between Landrace and Korean native pig (KNP). Using high-density porcine SNP chips,highly significant SNPs were identified on SSC12. Two candidate genes, LOC100523510 and LOC100621652, were subsequently selected within this region and further investigated. Within these candidate genes, five SNPs were identified and genotyped using the VeraCode GoldenGate assay. The results revealed that one SNP in the LOC100621652 gene and four SNPs in the LOC100523510 gene are highly associated with CW. These SNP markers can thus have significant applications for improving CW in KNP. However, the functions of these candidate genes are not fully understood and require further study.
참고문헌 (19)
- Aulchenko, Y. S., "Genomewide rapid association using mixed model and regression : a fast and simple method for genome-wide pedigree-based quantitative trait loci association analysis", Genetics, 177, pp.577~585 (2007)
- Balding, D. J., "A tutorial on statistical methods for population association studies", Nat. Rev. Genet, 7, pp.781~791 (2006)
- Cho, I. C., "QTL analysis of white blood cell, platelet and red blood cell-related traits in an F2 intercross between Landrace and Korean native pigs", Anim. Genet, 42, pp.621~626 (2011)
- 조수현, "재래돼지의 출하체중별 도체수율 및 육질특성", 한국동물자원과학회지, 49(4), pp.523~530 (2007), 한국동물자원과학회
- Cho, Y. M., "A study on growth characteristics of Korean native pig (KNP) and Landrace using individual growth curve parameters", J. Anim. Sci. & Technol, 43(6), pp.817~822 (2001)
- Ernst, C. W., "Molecular advances in QTL discovery and application in pig breeding", Trends Genet, 29(4), pp.215~224 (2013)
- Geldermann, H., "Genome-wide mapping of quantitative trait loci for fatness, fat cell characteristics and fat metabolism in three porcine F2 crosses", Genet. Sel. Evol, 42, pp.31~ (2010)
- Eung Woo Park, "A Large-Insert Porcine Library with Sevenfold Genome Coverage: a Tool for Positional Cloning of Candidate Genes for Major Quantitative Traits", Molecules and Cells, 16(1), pp.113~116 (2003), 한국분자세포생물학회
- Jin, S. K., "Physicochemical characteristics of longissimus muscle between the Korean native pig and Landrace", Korean J. Food Sci. Ani. Resour, 21(2), pp.142~148 (2001)
- E.H. Kim, "Detection of Mendelian and Parent-of-origin Quantitative Trait Loci in a Cross between Korean Native Pig and Landrace I. Growth and Body Composition Traits", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 20(5), pp.669~676 (2007), 아세아·태평양축산학회
- S.W. Kim, "QTL Scan for Meat Quality Traits Using High-density SNP Chip Analysis in Cross between Korean Native Pig and Yorkshire", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 24(9), pp.1184~1191 (2011), 아세아·태평양축산학회
- Tae-Hun Kim, "Identification of Quantitative Trait Loci (QTL) Affecting Teat Number in Pigs", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(9), pp.1210~1213 (2004), 아세아·태평양축산학회
- Kwon, O. S., "Preservation background and environmental status of Korean native black pigs", Symposium proceedings of preservation and utilization of Korean native black pigs, pp.3~20 (2006)
- H.K.Lee, "Detection of Imprinted Quantitative Trait Loci (QTL) for Growth Traits in Pigs", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 16(8), pp.1087~1092 (2003), 아세아·태평양축산학회
- Ma, J., "Genome-wide identification of quantitative trait loci for carcass composition and meat quality in a large-scale White Duroc X Chinese Erhualian resource population", Anim. Genet, 40(5), pp.637~647 (2009)
- -Lphenylalanine methyl ester, "Met. Based Drugs", 1998, 1~9
- Purcell, S., "PLINK: A tool set for whole-genome association and population-based linkage analyses", Am. J. Hum. Genet, 81, pp.559~575 (2007)
- Thomsen, H., "Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine", J. Anim. Sci, 82(8), pp.2213~2218 (2004)
- Yoo, C. K., "QTL analysis of back fat thickness and carcass pH in an F2 intercross between Landrace and Korean native pigs.", Mol. Biol. Rep, 39, pp.8327~8333 (2012)