음향적 흐름을 이용한 혈액형 분석을 위한 새로운 방법
A novel Method for Blood Typing using Acoustic Streaming
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.
Abstract
Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.
참고문헌 (14)
- 서인범, "미세 유체 분배 기법을 이용한 혈액형 검사 키트의 개발", Blood Research, 42(4), pp.12~12 (2007), 대한혈액학회
- T. Kline, "ABO, D Blood Typing and Subtyping Using Plug-Based Microfluidics", Analytical Chemistry, 80(16), pp.6190~6197 (2008)
- A. Nilghaz, "Red blood cell transport mechanisms in polyester thread-based blood typing devices", Analytical and Bioanalytical Chemistry, 408(5), pp.1365~1371 (2015)
- H. Ashiba, "Microfluidic chips for forward blood typing performed with a multichannel waveguide-mode sensor", Sensing and Bio-Sensing Research, 7, pp.121~126 (2016)
- T. Songjaroen, "Simultaneous forward and reverse ABO blood group typing using a paperbased device and barcode-like interpretation", Analytica Chimica Acta, 921, pp.67~76 (2016)
- N. Yeow, "Paper-based assay for red blood cell antigen typing by the indirect antiglobulin test", Analytical and Bioanalytical Chemistry, 408(19), pp.5231~5238 (2016)
- H. Zhang, "A dye-assisted paper-based point-of-care assay for fast and reliable blood grouping", Science Translational Medicine, 9(381), pp.eaaf9209~ (2017)
- A. Rezk, "Simple, low cost MHz-order acoustomicrofluidics using aluminium foil electrodes", Lab Chip, 14(11), pp.1802~1805 (2014)
- G. Destgeer, "Lamb Wave-Based Acoustic Radiation Force-Driven Particle Ring Formation Inside a Sessile Droplet", Analytical Chemistry, 88(7), pp.3976~3981 (2016)
- Amgad R. Rezk, "Lithography-free, crystal-based multiresonant Lamb waves for reconfigurable microparticle manipulation", Journal of Microelectronics, Electronic Components and Materials, 46(4), pp.176~182 (2016)
- R. Shilton, "Particle concentration and mixing in microdrops driven by focused surface acoustic waves", Journal of Applied Physics, 104(1), pp.014910~ (2008)
- Leslie Y. Yeo, "Surface Acoustic Wave Microfluidics", The Annual Review of Fluid Mechanics, 46, pp.379~406 (2013)
- L. Yeo, "Ultrafast microfluidics using surface acoustic waves", Biomicrofluidics, 3(1), pp.012002~ (2009)
- J. Nam, "Lamb wavebased blood coagulation test", Sensors and Actuators B:Chemical, 263, pp.190~195 (2018)