Comparing the mortality of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) caused by entomopathogenic bacteria and Serratia marcescens (Enterobacteriales: Enterobacteriaceae)
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of 4 × 108 pfu/mL in 2nd stage P. b. seulensis larvae and at 6 × 108 pfu/mL in 3rd stage P. b. seulensis larvae. In particular, mortality resulting from a combination of P. popilliae and S. marcescens was markedly increased in 2nd stage P. b. seulensis larvae. Therefore, we confirmed that mortality was increased when S. marcescens was infected together with other entomopathogenic bacteria, and that peroral infection itself can be fatal beyond certain concentrations
Abstract
To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of 4 × 108 pfu/mL in 2nd stage P. b. seulensis larvae and at 6 × 108 pfu/mL in 3rd stage P. b. seulensis larvae. In particular, mortality resulting from a combination of P. popilliae and S. marcescens was markedly increased in 2nd stage P. b. seulensis larvae. Therefore, we confirmed that mortality was increased when S. marcescens was infected together with other entomopathogenic bacteria, and that peroral infection itself can be fatal beyond certain concentrations
참고문헌 (15)
- Alejandra Bravo, "Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control", Toxicon, 49(4), pp.423~435 (2007), Elsevier BV[DOI]
- Carol R. Lauzon TGB, "Serratia marcescens as a bacterial pathogen of rhagoletis pomonella flies", Eur J Entomol, 100, pp.87~92 (2003)
- Mi Yeon Chung, "흰점박이꽃무지(Protaetia brevitarsis)의 일반성분 및 유해물질 분석", 생명과학회지, 23(5), pp.664~668 (2013), 한국생명과학회[DOI]
- Mi Yeon Chung, "식약용으로의 장수풍뎅이 유충 전처리 방법에 대한 연구", 생명과학회지, 23(3), pp.426~431 (2013), 한국생명과학회[DOI]
- F. E. El-Borai LWD, "Bionomics of a phoretic assciation between Paenibacillus sp. and the entomopathogenic nematode Steinernema diaprepesi", Journal of Nematology, 37, pp.18~25 (2005)
- Yulin Gao, "Bacillus thuringiensis Cry3Aa toxin increases the susceptibility of Crioceris quatuordecimpunctata to Beauveria bassiana infection", Journal of Invertebrate Pathology, 109(2), pp.260~263 (2012), Elsevier BV[DOI]
- Helen Harrison, "Paenibacillus Associated with Milky Disease in Central and South American Scarabs", Journal of Invertebrate Pathology, 76(3), pp.169~175 (2000), Elsevier BV[DOI]
- Kenichi Ishii, "Identification of a Serratia marcescens virulence factor that promotes hemolymph bleeding in the silkworm, Bombyx mori", Journal of Invertebrate Pathology, 117, pp.61~67 (2014), Elsevier BV[DOI]
- 곽규원, "Detection of Insect Pathogen Serratia marcescens in Protaetia brevitarsis seulensis (Kolbe) from Korea", International Journal of Industrial Entomology, 28(2), pp.25~31 (2014), 한국잠사학회
- 권은영, "흰점박이꽃무지(Protaetia brevitarsis)의 식품원료화를 위한 전처리 조건 확립", 한국식품영양과학회지, 42(3), pp.397~402 (2013), 한국식품영양과학회
- Lauzon C. R. BTG, "Serratia marcescens as a bacterial pathogen of rhagoletis pomonella flies(Diptera: Tephritidae)", Eur J Entomol, 87-92 (2003)
- Lee S, "First Report of Oryctes rhinoceros nudivirus (Coleoptera: Scarabaeidae) Causing Severe Disease in Allomyrina dichotoma in Korea", Journal of insect science, 15(1) (2015)[DOI]
- S. L. Murdoch, "The Opportunistic Pathogen Serratia marcescens Utilizes Type VI Secretion To Target Bacterial Competitors", Journal of Bacteriology, 193(21), pp.6057~6069 (2011), American Society for Microbiology[DOI]
- P. A. Noguera, "Detection of New cry Genes of Bacillus thuringiensis by Use of a Novel PCR Primer System", Applied and Environmental Microbiology, 76(18), pp.6150~6155 (2010), American Society for Microbiology[DOI]
- Renaud Stalinski, "Pre-selecting resistance against individual Bti Cry toxins facilitates the development of resistance to the Bti toxins cocktail", Journal of Invertebrate Pathology, 119, pp.50~53 (2014), Elsevier BV[DOI]