Apostichopus japonicas (Echinodermata; Holothuroidea)에서 온도 스트레스에 의한 Hsp90 및 Ferritin 유전자의 발현
The Expression of Hsp90 and Ferritin Genes under Thermal Stress in the Sea Cucumber (Apostichopus japonicas)
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
The Apostichopus japonicus is an important species in some Asia countries including Korea, China and Japan. The purpose of the present study was to investigate the differential gene expression of heat shock protein90 (Hsp90) and ferritin as a biomarker for the thermal stress during water temperature rising in the sea cucumber, A. japonicus. The A. japonicus (1.4 g) was cultured in incubator of separate temperature (15°C, 20°C, 25°C and 30°C) for each 0, 3, 6, 12, 24, 48 hours. The mRNA expression levels of Hsp90 and ferritin were examined using RT-PCR assay. Results showed that, the expression of Hsp90 mRNA was not significantly changed at 15°C. The expression of Hsp90 mRNA was significantly increased at high temperature such as 20°C and 25°C. Furthermore, Hsp90 mRNA was early increased at 25°C than 20°C. The ferritin mRNA was similar expression pattern with Hsp90. But, Hsp90 mRNA was more sensitive than ferritin mRNA at high thermal stress. These results indicate that Hsp90 and ferritin mRNAs were involved in the temperature changes response and may be play an important role in mediating the thermal stress in A. japonicas.
Abstract
The Apostichopus japonicus is an important species in some Asia countries including Korea, China and Japan. The purpose of the present study was to investigate the differential gene expression of heat shock protein90 (Hsp90) and ferritin as a biomarker for the thermal stress during water temperature rising in the sea cucumber, A. japonicus. The A. japonicus (1.4 g) was cultured in incubator of separate temperature (15°C, 20°C, 25°C and 30°C) for each 0, 3, 6, 12, 24, 48 hours. The mRNA expression levels of Hsp90 and ferritin were examined using RT-PCR assay. Results showed that, the expression of Hsp90 mRNA was not significantly changed at 15°C. The expression of Hsp90 mRNA was significantly increased at high temperature such as 20°C and 25°C. Furthermore, Hsp90 mRNA was early increased at 25°C than 20°C. The ferritin mRNA was similar expression pattern with Hsp90. But, Hsp90 mRNA was more sensitive than ferritin mRNA at high thermal stress. These results indicate that Hsp90 and ferritin mRNAs were involved in the temperature changes response and may be play an important role in mediating the thermal stress in A. japonicas.
참고문헌 (43)
- Basu N., "Heat shock protein genes and their functional significance in fish", Gene, 295, pp.173~183 (2002)
- Colinet H., "Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster", FEBS J, 277, pp.174~185 (2010)
- Dong YW., "Effects of different thermal regimes on growth and physiological performance of the sea cucumber Apostichopus japonicus Selenka", Aquaculture, 275, pp.329~334 (2008)
- Fangue NA., "Intraspecific variation in thermal tolerance and heat shock protein gene expression in common killifish, Fundulus heteroclitus", J. Exp. Biol, 209, pp.2859~2872 (2006)
- Feder ME., "Heat-shock proteins, molecular chaperones, and the stress response : evolutionary and ecological physiology", Annu. Rev. Physiol, 61, pp.243~282 (1999)
- Gagnaire B., "Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas(Thunberg)", Fish Shellfish Immunol, 20, pp.536~547 (2006)
- Galea-Lauri J., "Increased heat shock protein 90(hsp90)expression leads to increased apoptosis in the monoblastoid cell line U937 following induction with TNF-alpha and cycloheximide : a possible role in immunopathology", J. Immunol, 157, pp.4109~4118 (1996)
- Georgopoulos C., "Role of the major heat shock proteins as molecular chaperones", Annu. Rev. Cell Biol, 9, pp.601~634 (1993)
- Harrison PM., "Ferritins: molecular properties, iron storage function and cellular regulation", BioChim. Biophys. Acta, 1275, pp.161~203 (1996)
- Heckathorn SA., "The small, methionine-rich chloroplast heat-shock protein protects photosystem II electron transport during heat stress", Plant Physiol, 116, pp.439~444 (1998)
- Imai J., "Role of HSP90 in salt stress tolerance via stabilization and regulation of calcineurin", Mol. Cell. Biol, 20, pp.9262~9270 (2000)
- Jakob U., "Transient interaction of Hsp90 with early unfolding intermediates of citrate synthase", J. Biol. Chem, 270, pp.7288~7294 (1995)
- Kakhlon O., "Repression of the heavy ferritin chain increases the labile iron pool of human K562 cells", Biochem. J, 356, pp.311~316 (2001)
- Konijn AM., "The cellular labile iron pool and intracellular ferritin in K562 cells", Blood, 94, pp.2128~2134 (1999)
- Kregel KC., "Heat shock proteins : modifying factors in physiological stress responses and acquired thermotolerance", J. Appl. Physiol, 92, pp.2177~2186 (2002)
- Li BQ., "Effects of temperature on respiration and excretion of sea cucumber Apostichopus japonicas", Oceanol. Limnol. Sin, 33, pp.182~187 (2002)
- Li FX., "Study on aestivating habit of sea cucumber Apostichopus japonicus Selenka. II. Ecological characteristic of aestivation", J. Fish. Sci. China, 3, pp.49~57 (1996)
- Li P., "Molecular cloning, mRNA expression, and characterization of HSP90 gene from Chinese mitten crab Eriocheir japonica sinensis", Comp. Biochem. Physiol. B, Biochem. Mol. Biol, 153, pp.229~235 (2009)
- Liao Y., "FAUNA SINICA: phylum echinodermata" (1997), class holothuroidea Science Press
- Liu YH., "Study on aestivating habit of sea cucumber Apostichopus japonicus. I. Ecological characteristic of aestivation", J. Fish. Sci. China, 3, pp.41~48 (1996)
- Logue J., "Heat injury and resistance adaptation in fish", J. Therm. Biol, 20, pp.191~197 (1995)
- Morimoto RI., "Regulation of the heat shock transcriptional responses : cross talk between a family of heat shock factors, molecular chaperones, and negative regulators", Genes Dev, 12, pp.3788~3796 (1998)
- Murtha JM., "Characterization of the heat shock response in mature zebrafish(Danio rerio)", Exp. Gerontol, 38, pp.683~691 (2003)
- Palmisano AN., "Tissue specific induction of hsp90 mRNA and plasma cortisol response in chinook salmon following heat shock, seawater challenge, and handling challenge", Mar. Biotechnol, 2, pp.329~338 (2000)
- Park K., "Biological and molecular responses of Chironomus riparius(Diptera, Chironomidae)to herbicide 2, 4-D(2, 4-dichlorophenoxyacetic acid)", Comp. Biochem. Physiol. C-Toxicol. Pharmacol, 151, pp.439~446 (2010)
- Parsell D., "The function of heat-shock proteins in stress tolerance : degradation and reactivation of damaged proteins", Annu. Rev. Genet, 27, pp.437~496 (1993)
- Picard V., "Overexpression of the ferritin H subunit in cultured erythroid cells changes the intracellular iron distribution", Blood, 87, pp.2057~2064 (1996)
- Picard V., "Role of ferritin in the control of the labile iron pool in murine erythroleukemia cells", J. Biol. Chem, 273, pp.15382~15386 (1998)
- Queitsch C., "Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis", Plant Cell, 12, pp.479~492 (2000)
- Richter K., "Hsp90 : chaperoning signal transduction", J. Cell. Physiol, 188, pp.281~290 (2001)
- Sathiyaa R., "Cortisol modulates hsp90 mRNA expression in primary cultures of trout hepatocytes", Comp. Biochem. Physiol. B, Biochem. Mol. Biol, 129, pp.679~685 (2001)
- Soetaert A., "Molecular impact of propiconazole on Daphnia magna using a reproduction-related cDNA array", Comp. Biochem. Physiol. C, Toxicol. Pharmacol, 142, pp.66~76 (2006)
- Song L., "The cDNA cloning and mRNA expression of heat shock protein 70 gene in the haemocytes of bay scallop(Argopecten irradians, Lamarck 1819)responding to bacteria challenge and naphthalin stress", Fish Shellfish Immunol, 21, pp.335~345 (2006)
- Theil EC., "Ferritin : structure, gene regulation, and cellular function in animals, plants, and microorganisms", Annu. Rev. Biochem, 56, pp.289~315 (1987)
- Tsuji Y., "Coordinate transcriptional and translational regulation of ferritin in response to oxidative stress", Mol. Cell. Biol, 20, pp.5818~5827 (2000)
- Wang FY., "Effects of acute temperature or salinity stress on the immune response in sea cucumber, Apostichopus japonicus", Comp. Biochem. Physiol. A-Mol. Integr. Physiol, 151, pp.491~498 (2008)
- Wu LT., "Characterization of heat shock protein 90 in the shrimp Metapenaeus ensis : evidence for its role in the regulation of vitellogenin synthesis", Mol. Reprod. Dev, 75, pp.952~959 (2008)
- Xu D., "Polymorphisms of heat shock protein 90(Hsp 90)in the sea cucumber Apostichopus japonicas and their association with heat-resistance", Fish Shellfish Immunol, 41, pp.428~436 (2014)
- Xu Q., "Molecular cloning of heat shock protein 60(PtHSP60)from Portunus trituberculatus and its expression response to salinity stress", Cell Stress Chaperone, 17, pp.589~601 (2012)
- Yang HS., "Metabolic characteristics of sea cucumber Apostichopus japonicus(Selenka)during aestivation", J. Exp. Mar. Biol. Ecol, 330, pp.505~510 (2006)
- You L., "The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge", Fish Shellfish Immunol, 35, pp.343~350 (2013)
- Zhang P., "Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicas. Comp. Biochem. Physiol", Part D Genomics Proteomics, 8, pp.194~200 (2013)
- Zhao H., "The molecular characterization and expression of heat shock protein 90(Hsp90)and 26(Hsp26)cDNAs in sea cucumber(Apostichopus japonicas)", Cell Stress Chaperone, 16, pp.481~493 (2011)