고지방 식이에 의한 흰쥐의 지방간증에서 오미자 추출물의 간보호 효과
Hepatoprotective effect of Schisandra chinensis on high-fat diet-induced fatty liver in rats
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초록
The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD＋SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.
Abstract
The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD＋SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.
참고문헌 (34)
- Albu J, "Obesity solutions: report of a meeting", Nutr Rev, 55, pp.150~156 (1997)
- Asselah T, "Steatosis in chronic hepatitis C: why does it really matter?", Gut, 55, pp.123~130 (2006)
- 조영제, "오미자(Schizandra chinensis Baillon) 추출물의 생리활성", Journal of the Korean Society for Applied Biological Chemistry, 50(3), pp.198~203 (2007), 한국응용생명화학회
- Frings CS, "Improved determination of total serum lipids by the sulfophospho- vanillin reaction", Clin Chem, 18, pp.673~674 (1972)
- Grundy SM, "Multifactorial causation of obesity: implications for prevention", Am J Clin Nutr, 67, pp.563S~572S (1998)
- Hosseinian FS, "Measurement of anthocyanins and other phytochemicals in purple wheat", Food Chem, 109, pp.916~924 (2008)
- Ikeya Y, "The constituents of Schisandra chinensis Baill. Ⅹ Ⅴ. Isolation and structure determination of two new lignans, gomisin S and gomisin T", Chem Pharm Bull, 36, pp.3974~3981 (1988)
- Jo SH, "In vitro and in vivo anti-hyperglycemic effects of Omija (Schizandra chinensis) fruit", Int J Mol Sci, 12, pp.1359~1370 (2011)
- 김대중, "천년초의 열수 및 에탄올 추출물의 항산화 및 항비만 활성", 한국식품저장유통학회지, 18(3), pp.366~373 (2011), 한국식품저장유통학회
- 김현구, "오미자 추출물의 추출특성 및 항산화 효과", 한국식생활문화학회지, 19(5), pp.484~490 (2004), 한국식생활문화학회
- Kim SH, "Anti-apoptotic and hepatoprotective effects of gomisin A on fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice", J Pharmacol Sci, 106, pp.225~228 (2008)
- 김수민, "녹차와 오미자 첨가 육제품(양념계육 및 패티)이 흰쥐의 체중, 혈청지질 및 간조직에 미치는 영향", 한국식품영양과학회지, 34(7), pp.980~986 (2005), 한국식품영양과학회
- Kim YS, "Drug treatment of obesity", Kor Endocrine Soc, 16, pp.9~15 (2001)
- Kwon TD, "Effects of polyphenol or vitamin C ingestion on antioxidative activity during exercise in rats", Kor J Physical Education, 3, pp.891~899 (2001)
- 이혜성, "함초 열수추출물의 섭취가 비만유도 흰쥐의 항비만 효과에 미치는 영향", 한국식품영양과학회지, 41(7), pp.950~956 (2012), 한국식품영양과학회[DOI]
- 이성환, "陽陵泉 五味子약침이 고지방식이로 유발된 고지혈증 흰쥐에 미치는 영향", 대한침구의학회지, 28(2), pp.143~153 (2011), 대한침구의학회
- 이수정, "홍마늘 복합물이 고지방-콜레스테롤 식이성 흰쥐의 분변 지질 함량 및 간 조직의 항산화 효소 활성에 미치는 영향", 한국식품영양과학회지, 42(1), pp.17~25 (2013), 한국식품영양과학회
- Lee YK, "Effect of omija (Schisandra chinensis Baillon) methanol extract on benzo(a)pyrene induced hepatotoxicity in rats", J East Asian Soc Dietary Life, 5, pp.21~27 (1995)
- Lim SC, "Effects of Schisandra chinensis BAILL on lipid lowering and antioxidant in hyperlipidemic rat", Korean J Plant Resources, 7, pp.216~221 (2004)
- Marchesini G, "Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome", Hepatology, 37, pp.917~923 (2003)
- Na M, "Fatty acid synthase inhibitory activity of dibenzocyclooctadiene lignans isolated from Schisandra chinensis", Phytother Res, 24, pp.225~228 (2010)
- Ock ES, "Effect of Schisandra chinensis extract in hyperlipidemic rats", J Korean Soc Food Sci Nutr, 24, pp.658~662 (1995)
- Oh SL, "Composition of free sugars free amino acids non volatile organic acids and tannins in the extracts of L. chinensis M., A. acutiloba K., S. chinensis B. and A. sessiliflorum S", Korean J Food Sci Technol, 22, pp.76~81 (1990)
- Ozsoy N, "In vitro antioxidant activity of Amaranthus lividus L", Food Chem, 116, pp.867~872 (2009)
- Park HJ, "Anti-obesity effect of Schisandra chinensis in 3T3-L1 cells and high fat diet-induced obese rats", Food Chem, 134, pp.227~234 (2012)
- Park HS, "Pharmacological therapy of obesity", Kor J Soc Study Obes, 10, pp.118~127 (2001)
- 박성혜, "기능성 식품으로의 활용을 위한 한약자원에 관한 연구. 1. 오미자 열수추출물이 흰쥐의 국소 뇌혈류량과 혈압에 미치는 영향", 한국식품영양과학회지, 33(1), pp.34~40 (2004), 한국식품영양과학회
- 박승하, "Prevalence and risk factors of non-alcoholic fatty liver disease among Korean adults", JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 21, pp.138~143 (2006), BLACKWELL PUBLISHING
- 성기천, "천연 오미자 추출물의 약리, 화학적 특성 및 분석", 韓國油化學會誌, 28(3), pp.290~298 (2011), 한국유화학회
- Uchiyama M, "Determination of malondialdehyde precursor in tissues by TBA test", Anal Biochem, 86, pp.271~278 (1978)
- Visscher TL, "The public health impact of obesity", Annu Rev Public Health, 22, pp.355~375 (2001)
- Yagi K, "Assay for blood plasma or serum, In Method in Enzymology", pp.328~331 (1984), Academic Press
- Yim SY, "Gomisin N isolated from Schisandra chinensis significantly induces anti-proliferative and pro-apoptotic effects in hepatic carcinoma", Mol Med Rep, 2, pp.725~732 (2009)
- Se Na Yun, "Wild Ginseng Prevents the Onset of High-Fat Diet Induced Hyperglycemia and Obesity in ICR Mice", Archives of Pharmacal Research, 27(7), pp.790~796 (2004), 대한약학회