소양호에 존재하는 새로운 Flavobacterium의 분포와 특징
Characterization and Distribution of Various Flavobacterium sp. in Lake Soyang
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
우리나라 최대 담수호인 소양호에 존재하는 미생물 중Flavobacterium을 분리하여 그 특징을 조사하였다. 그 중에서 97%와 98% 사이의 상동성을 지니는 Flavobacterium을대상으로 21종을 선택하였으며, 그 균주들의 생리학적, 생태학적 위치를 살펴보았다. 그 결과, 소양호에 존재하는 21종의 분리된 Flavobacterium은 4개의 주된 node 에 위치하였으며, 그 중에서 대부분이 하나의 group에 속하여 있었다. 각 Flavoacterium 21종의 생리적인 특징은기존의 보고된 Flavoacterium들과는 서로 다른 특징을 지니지만, total fatty acid의 분석결과 iso C15:0과 summed feature 3 (comprised C16:1 ω7c and/or C16:1 ω6c)가 주요 성분으로 전형적인 Flavobacterium의 특징을 지닌 것으로 나타났다. 따라서 소양호내에는 유전적 유연관계가 있는 아변종의 Flavobacterium이 존재하며, 기존에 알려진 종들과는 다른 형질을 가지고 있는 것으로 보아 소양호 생태계의 생물 종 다양성이 유지되고 있는 것으로 판단된다.
Abstract
In this study, samples were collected from Lake Soyang in Kangwon-do in order to isolate novel Flavobacterium sp. strains. Totally, 21 strains of Flavobacterium showing 97%~98%similarity in 16S rRNA were selected and thoroughly investigated individual characteristics and ecological differences. As results, we could categorize Flavobacterium isolated from Lake Soyang into four major node groups, where most of Flavobacterium belonged to single group. Next, fatty acid analyses were performed demonstrating similar pattern of the majority of fatty acids as either iso C15:0 or summed feature 3 (comprised C16:1 ω7c and/or C16:1 ω6c) of the other Flavobacterium. However, other phenotypic data were different from the other Flavobacterium sp. Group. Our data showed that genetically related species of Flavobacterium have been distributed in Lake Soyang. Those Flavobacterium strains were phenotypically different from previously reported genus of Flavobacterium species. Taken together, we speculated that isolated Flavobacterium strains from Lake Soyang might be ecologically important members to maintain ecosystem.
참고문헌 (19)
- 이은주, "소양호 지류에서의(상걸리)식 물플랑크톤 군집구조(1984~1997)", 한국육수학회지, 31, pp.119~128 (1988)
- Ahn TS., "Factor analysis of the environmental impact on microbial populations in Soyang Lake", Kor. J. Lim, 19, pp.11~17 (1989)
- Bernardet JF., "The Genus Flavobacterium, In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7", pp.481~531 (2006), Springer
- Bernardet JF., "Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family", Int. J. Syst. Evol. Microbiol, 52, pp.1049~1070 (2002)
- Choi SI., "The Cellulose degradation and distribution of attached bacteria on cellulose film in lake Soyang", Kor. Jour. Microbiol, 31, pp.355~464 (1993)
- 최연규, "국내 호소에서 autotrophic picoplankton의 밀도 및 식물플랑크톤 생물량에 대한 기여도", 생태와 환경, 34(3), pp.141~152 (2001), 한국하천호수학회
- Chun J., "EzTaxon : a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences", Int. J. Syst. Evol. Microbiol, 57, pp.2259~2261 (2007)
- Fu Y., "Flavobacterium beibuense sp. nov., isolated from marine sediment", Int. J. Syst. Evol. Microbiol, 61, pp.205~209 (2011)
- Kim MW., "Distribution of bacterial biomass in the water column of Soyang Lake", Kor. Jour. Microbiol, 27, pp.130~138 (1989)
- Kim SJ., "Diurnal variation for the bacterial activities affected by the phytoplankton changes in a branch of lake Soyang", Kor. Jour. Microbiol, 32, pp.580~585 (1994)
- Miyashita M., "Flavobacterium algicola sp. nov., isolated from marine algae", Int. J. Syst. Evol. Microbiol, 60, pp.344~348 (2010)
- Mudarris M., "Flavobacterium scophthalmum sp. nov., a pathogen of turbot(Scophthalmus maximus L. )", Int. J. Syst. Bacteriol, 44, pp.447~453 (1994)
- Pérez-Pascual D., "Spreading versus biomass production by colonies of the fish pathogen Flavobacterium psychrophilum : role of nutrient concentration", Int. Microbiol, 12, pp.207~214 (2009)
- Pilarski F., "Isolation and characterization of Flavobacterium columnare(Bernardet et al. 2002)from four tropical fish species in Brazil", Braz. J. Biol, 68, pp.409~414 (2008)
- Saitou N., "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Tamura K., "MEGA4 : Molecular Evolutionary Genetics Analysis(MEGA)software version 4. 0", Mol. Biol. Evol, 24, pp.1596~1599 (2007)
- Tiirola M., "Diagnosis of flavobacteriosis by direct amplification of rRNA genes", Dis. Aquat. Organ, 51, pp.93~100 (2002)
- Xu M., "Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier", Int. J. Syst. Evol. Microbiol, 61, pp.20~24 (2011)
- Yoon JH., "Flavobacterium ponti sp. nov., isolated from seawater", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61, pp.81~85, SOC GENERAL MICROBIOLOGY