대한민국 청주 지역사회의 CTX-M Extended-spectrum β-lactamase 생성 대장균주의 확산
Spread of CTX-M Extended-spectrum β-lactamase Producing Escherichia coli in the Community in Chungcheong Area, Korea
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초록
This study was designed to evaluate the prevalence of ESBL genes and monitor antimicrobial resistance pattern in Escherichia coli , isolated from a hospital and a community. We tested 200 E. coli strains isolated in the hospitals and community in Chungcheong area from January to March 2012. Antimicrobial susceptibilities were tested by using the disk diffusion method. A search for ESBL genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. An Epidemiologic study was performed by repetitive extragenic palindromic sequence-based PCR (REP-PCR). The percentage of ESBLproducing isolates was 17% for hospital associated E. coli and 11% for community associated E. coli. The ESBL gene sequencing results showed that the most common ESBL in E. coli was CTX-M-14 (19/28), followed by CTX-M-15 (9/28). The REP-PCR study also showed the genetic diversity, but there was no difference between the hospital and community associated E. coli. In this study, the most common types of class A ESBLs identified were CTX-M in the hospital and the community in Chungcheong area. ESBL-producing E. coli isolates showed diverse clonality.
Abstract
This study was designed to evaluate the prevalence of ESBL genes and monitor antimicrobial resistance pattern in Escherichia coli , isolated from a hospital and a community. We tested 200 E. coli strains isolated in the hospitals and community in Chungcheong area from January to March 2012. Antimicrobial susceptibilities were tested by using the disk diffusion method. A search for ESBL genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. An Epidemiologic study was performed by repetitive extragenic palindromic sequence-based PCR (REP-PCR). The percentage of ESBLproducing isolates was 17% for hospital associated E. coli and 11% for community associated E. coli. The ESBL gene sequencing results showed that the most common ESBL in E. coli was CTX-M-14 (19/28), followed by CTX-M-15 (9/28). The REP-PCR study also showed the genetic diversity, but there was no difference between the hospital and community associated E. coli. In this study, the most common types of class A ESBLs identified were CTX-M in the hospital and the community in Chungcheong area. ESBL-producing E. coli isolates showed diverse clonality.
참고문헌 (15)
- CLSI, "Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. CLSI document M100-S20", Clinical and Laboratory Standards Institute, 52~53 (2010)
- Hong SG, "Molecular characteristics of extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae", Korean J Clin Pathol, 21, pp.495~504 (2001)
- Jeong SH, "Molecular characterization of extended-spectrum β-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey", J Clin Microbiol, 42, pp.2902~2906 (2004)
- Kang CI, "Clinical features and outcome of community-onset bloodstream infections caused by extended-spectrum beta-lactamase-producing Escherichia coli", Eur J Clin Microbiol Infect Dis, 27, pp.85~88 (2008)
- Kim J, "CTX-M and SHV-12 beta-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumonia collected from 3 university hospitals within Korea", FEMS Microbiol Lett, 245, pp.93~98 (2005)
- Ko KS, "Prevalence and characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated in Korean hospitals", Diagn Microbiol Infect Dis, 61, pp.453~459 (2008)
- Lee BY, "Detection of extended- spectrum β-lactamase-producing Escherichia coli and Klebsiella spp. with the Vitek GNS 121 card", Korean J Clin Pathol, 21, pp.350~354 (2001)
- 이학민, "Frequency of Extended-spectrum b-lactamase (ESBL) and AmpC b-lactamase Genes in Escherichia coli and Klebsiella pneumoniae over a Three-year Period in a University Hospital in Korea", Annals of Laboratory Medicine, 30(6), pp.616~623 (2010), 대한진단검사의학회
- Nataro JP, "Diarrheagenic Escherichia coli", Clin Microbiol Rev, 11, pp.142~201 (1998)
- Park SH, "Molecular epidemiology of extended-spectrum β-lactamase-producing Escherichia coli in the community and hospital in Korea: emergence of ST131 producing CTX-M-15", BMC Infect Dis, 12, pp.149~160 (2012)
- 박용정, "Prevalence of the Extended-Spectrum β-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli", Annals of Laboratory Medicine, 29(3), pp.218~223 (2009), 대한진단검사의학회
- Rodríguez-Baño J, "Community-onset bacteremia due to extended-spectrum beta- lactamase-producing Escherichia coli : risk factors and prognosis", Clin Infect Dis, 50, pp.40~48 (2010)
- Shannon KP, "Increasing resistance to antimicrobial agents of Gram-negative organisms isolated at a London teaching hospital, 1995-2000", J Antimicrob Chemother, 53, pp.818~825 (2004)
- Song W, "CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum β-lactamase in clinical isolates of Escherichia coli from Korea", J Med Microbiol, 58, pp.261~266 (2009)
- 양병선, "Multiplex PCR을 이용한 Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의 Quinolone 내성 qnr유전자 검출", 대한임상검사과학회지, 39(3), pp.161~166 (2007), 대한임상검사과학회