본문 바로가기
KERD
논문KCI 등재

Control of Ca^(2+)- Influx by Ca^(2+)/Calmodulin Dependent Protein Kinase II in the Activation of Mouse Eggs

강다원|윤숙영|배인하
한국발생생물학회 (The Korea Society Of Developmental Biology)|2011.03|발생과 생식 (DEVELOPMENT & REPRODUCTION)|제15권 제1호|pp. 31-39

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

intracellular Ca^(2+)-concentration ([Ca^(2+)]i )Ca^(2+)/calmodulin dependent protein kinase IICa^(2+) influxegg activation
주제분류생물학

초록

Change in intracellular Ca^(2+)-concentration ([Ca^(2+)]i) is an essential event for egg activation and further development. Ca^(2+) ion is originated from intracellular Ca^(2+)-store via inositol 1,4,5-triphosphate receptor and/or Ca^(2+) influx via Ca^(2+) channel. This study was performed to investigate whether changes in Ca^(2+)/calmodulin dependent protein kinase II (CaM KII) activity affect Ca^(2+) influx during artificial egg activation with ethanol using Ca^(2+) monitoring system and whole-cell patch clamp technique. Under Ca^(2+) ion-omitted condition, Ca^(2+)- oscillation was stopped within 30 min post microinjection of porcine sperm factor, and ethanol-induced Ca^(2+) increase was reduced. To investigate the role of CaM KII known as an integrator of Ca^(2+)- oscillation during mammalian egg fertilization, CaM KII activity was tested with a specific inhibitor KN-93. In the eggs treated with KN-93, ethanol failed to induce egg activation. In addition, KN-93 inhibited inward Ca^(2+) current (I_(ca)) in a time-dependent manner in whole-cell configuration. Immunostaining data showed that the voltage-dependent Ca^(2+) channels were distributed along the plasma membrane of mouse egg and 2-cell embryo. From these results, we suggest that Ca^(2+) influx during fertilization might be controlled by CaM KII activity.

Abstract

Change in intracellular Ca^(2+)-concentration ([Ca^(2+)]i) is an essential event for egg activation and further development. Ca^(2+) ion is originated from intracellular Ca^(2+)-store via inositol 1,4,5-triphosphate receptor and/or Ca^(2+) influx via Ca^(2+) channel. This study was performed to investigate whether changes in Ca^(2+)/calmodulin dependent protein kinase II (CaM KII) activity affect Ca^(2+) influx during artificial egg activation with ethanol using Ca^(2+) monitoring system and whole-cell patch clamp technique. Under Ca^(2+) ion-omitted condition, Ca^(2+)- oscillation was stopped within 30 min post microinjection of porcine sperm factor, and ethanol-induced Ca^(2+) increase was reduced. To investigate the role of CaM KII known as an integrator of Ca^(2+)- oscillation during mammalian egg fertilization, CaM KII activity was tested with a specific inhibitor KN-93. In the eggs treated with KN-93, ethanol failed to induce egg activation. In addition, KN-93 inhibited inward Ca^(2+) current (I_(ca)) in a time-dependent manner in whole-cell configuration. Immunostaining data showed that the voltage-dependent Ca^(2+) channels were distributed along the plasma membrane of mouse egg and 2-cell embryo. From these results, we suggest that Ca^(2+) influx during fertilization might be controlled by CaM KII activity.

참고문헌 (37)

  1. Basavappa S, "Activation of calcium channels by cAMP in STC-1 cells is dependent upon Ca2+ calmodulin-dependent protein kinase II", Biochem Biophys Res Commun, 254, pp.699~702 (1999)
  2. Blancato JK, "Effect of calcium-modifying drugs on mouse in vitro fertilization and preimplantation development", Int J Fertil, 35, pp.171~176 (1990)
  3. Bourinet E, "Endogenous Xenopus-oocyte Ca-channels are regulated by protein kinases A and C", FEBS Lett, 299, pp.5~9 (1992)
  4. Ducibella T, "The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development", Dev Biol, 315, pp.257~279 (2008)
  5. Ducibella T, "Egg- to-embryo transition is driven by differential responses to Ca(2+) oscillation number", Dev Biol, 250, pp.280~291 (2002)
  6. Dupont G, "Link between fertilization-induced Ca2+ oscillations and relief from metaphase II arrest in mammalian eggs: a model based on calmodulin-dependent kinase II activation", Biophys Chem, 72, pp.153~167 (1998)
  7. Dupont G, "CaM kinase II as frequency decoder of Ca2+ oscillations", Bioessays, 20, pp.607~610 (1998)
  8. Emerson M, "Characterization and functional significance of calcium transients in the 2-cell mouse embryo induced by an autocrine growth factor", J Biol Chem, 275, pp.21905~21913 (2000)
  9. Hudmon A, "CaMKII tethers to L-type Ca2+ channels, establishing a local and dedicated integrator of Ca2+ signals for facilitation", J Cell Biol, 171, pp.537~547 (2005)
  10. Ito J, "The role of calcium", calmodulin-dependent protein kinase II on the inactivation of MAP kinase and p34cdc2 kinase during fertilization and activation in pig oocytes, 2004, pp.128, 409~415
  11. 윤숙영, "Inositol 1,4,5-trisphosphate receptor 1, a widespread Ca2+ channel, is a novel substrate of polo-like kinase 1 in eggs", DEVELOPMENTAL BIOLOGY, 320(2), pp.402~413 (2008), ACADEMIC PRESS INC ELSEVIER SCIENCE
  12. (i) oscillations in mouse zygotes and function of the inositol 1,4,5-trisphosphate receptor-1, "Dev Biol", 2004, 94~109
  13. Kang D, "Acetylcholine increases Ca2+ influx by activation of CaMKII in mouse oocytes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 360, pp.476~482 (2007), ACADEMIC PRESS INC ELSEVIER SCIENCE
  14. Kline D, "Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg", Dev Biol, 149, pp.80~89 (1992)
  15. Knott JG, "Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca2+ oscillations", Dev Biol, 296, pp.388~395 (2006)
  16. Lacinova L, "Voltage-dependent calcium channels", Gen Physiol Biophys, 24(1), pp.1~78 (2005)
  17. Lawitts JA, "Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium", Biol Reprod, 45, pp.245~251 (1991)
  18. 윤숙영, "Studies on Ca2+-channel distribution in maturation arrested mouse oocyte", MOLECULAR REPRODUCTION AND DEVELOPMENT, 69, pp.174~185, WILEY-LISS
  19. Lorca T, "Ca2+ is involved through type II calmodulin-dependent protein kinase in cyclin degradation and exit from metaphase", Biochim Biophys Acta, 1223, pp.325~332 (1994)
  20. Lorca T, "Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs", Nature, 366, pp.270~273 (1993)
  21. Lu HK, "Ca(2+)-dependent activation of T-type Ca2+ channels by calmodulin- dependent protein kinase II", Am J Physiol, 267, pp.183~189 (1994)
  22. Madgwick S, "Calmodulin- dependent protein kinase II, and not protein kinase C, is sufficient for triggering cell-cycle resumption in mammalian eggs", J Cell Sci, 118, pp.3849~3859 (2005)
  23. 윤숙영, "Comparison of Ca2+ and CaMKII responses in IVF and ICSI in the mouse", MOLECULAR HUMAN REPRODUCTION, 13(4), pp.265~272, OXFORD UNIV PRESS
  24. Markoulaki S, "Oscillatory CaMKII activity in mouse egg activation", Dev Biol, 258, pp.464~474 (2003)
  25. Mattioli M, "Maturation of pig oocytes: observations on membrane potential", Biol Reprod, 43, pp.318~322 (1990)
  26. Miyazaki S, "Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs", Dev Biol, 118, pp.259~267 (1986)
  27. Powers RD, "Changes in mouse oocyte membrane potential and permeability during meiotic maturation", J Exp Zool, 221, pp.365~371 (1982)
  28. Rogers NT, "The absence of a Ca(2+) signal during mouse egg activation can affect parthenogenetic preimplantation development, gene expression patterns, and blastocyst quality", Reproduction, 132, pp.45~57 (2006)
  29. Saunders CM, "PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development", Development, 129, pp.3533~3544 (2002)
  30. Schulman H, "Activity-dependent regulation of calcium", calmodulin-dependent protein kinase II localization, 2004, pp.24, 8399~8403
  31. Schultz RM, "Molecular basis of mammalian egg activation", Curr Top Dev Biol, 30, pp.21~62 (1995)
  32. Sumi M, "The newly synthesized selective Ca2+", calmodulin dependent protein kinase II inhibitor KN-93 reduces dopamine contents in PC12h cells, 1991, pp.181, 968~975
  33. Tatone C, "Biochemical and biological effects of KN-93, an inhibitor of calmodulin-dependent protein kinase II, on the initial events of mouse egg activation induced by ethanol", J Reprod Fertil, 115, pp.151~157 (1999)
  34. Tsien RW, "Calcium channels, stores, and oscillations", Annu Rev Cell Biol, 6, pp.715~760 (1990)
  35. Winston NJ, "Calmodulin-dependent protein kinase II is activated transiently in ethanol-stimulated mouse oocytes", Dev Biol, 170, pp.350~352 (1995)
  36. 윤숙영, "Human sperm devoid of PLC, zeta 1 fail to induce Ca2+ release and are unable to initiate the first step of embryo development", JOURNAL OF CLINICAL INVESTIGATION, 118(11), pp.3671~3681, AMER SOC CLINICAL INVESTIGATION INC
  37. Yoshida S, "Action potentials dependent on monovalent cations in developing mouse embryos", Dev Biol, 110, pp.200~206 (1985)

관련 논문AI추천

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization엄진희, 윤숙영, Jung Ah Yoon, Miseon Park한국발생생물학회 · 발생과 생식2020 · 제24권 제4호Production of Intracellular Calcium Oscillation by Phospholipase C Zeta Activation in Mammalian Eggs강다원, 윤숙영한국발생생물학회 · 발생과 생식2011 · 제15권 제3호Role of Type 1 Inositol 1,4,5-triphosphate Receptors in Mammalian Oocytes윤숙영한국발생생물학회 · 발생과 생식2019 · 제23권 제1호Phospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic developmentBouchra Ghazi, Modou Mamoune Mbaye, Moncef Benkhalifa, Noureddine Louanjli, Soukaina Azil대한산부인과학회 · Obstetrics & Gynecology Science2024 · 제67권 제5호Adenosine Receptors Mediated Intracellular Calcium in Cumulus Cells Involved in the Maintenance of First Meiotic Arrest전용필, 황희경한국발생생물학회 · 발생과 생식2013 · 제17권 제2호Prostaglandins Involving in Blastocyst Development through Calcium Mediated Signaling Pathway권혁찬, 이승재, 이자명, 전용필한국발생생물학회 · 발생과 생식2011 · 제15권 제1호

이 간행물의 다른 논문 (8건)

Expression of Wee1 Gene in the Ascidian, Halocynthia roretzi Embryo김길중, 함화서, 이원영pp. 1-7Dynamic Study of Tetrahymena pyriformis Growth and Reproduction in Aerobic and Anaerobic Conditions유은순pp. 9-15Prostaglandins Involving in Blastocyst Development through Calcium Mediated Signaling Pathway전용필, 권혁찬, 이승재, 이자명pp. 17-24No Relevance of NF-κB in the Transcriptional Regulation of Human Nanog Gene in Embryonic Carcinoma Cells이영희, 김영은, 박정아, 석현정pp. 25-30Comparative Analysis for In Vitro Differentiation Potential of Induced Pluripotent Stem Cells, Embryonic Stem Cells, and Spermatogonial Stem Cells into Germ-lineage Cells이현정, 고영은, 고정재, 이동율, 도정태, 김형준, 조중현pp. 41-52
원문 보기