Effect of Nitric Oxide on ADP-ribose Pyrophosphatase Activity
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Abstract
Background: ADP-ribosyl pyrophosphatases (ADPRase) has been known to catalyze the hydrolysis of ADP-ribose to ribose-5-phosphate and AMP. The role of ADPRase has been suggested to sanitize the cell by removing potentially toxic ADP-ribose. In this study, we examined the effect of nitric oxide on ADPRase activity in macrophages. Methods: ADPRase activity was measured in NO-inducing J774 cells. For in vitro experiments, recombinant human ADPRase was prepared in bacteria. Results: ADPRase activity was increased by the treatment of exogenous NO generating reagent, sodium nitroprusside (SNP), in J774 cells. The increased ADPRase activity was mediated by the post-translational modification, likely to cause cADP-ribosylation via nitrosylation of cysteine residue on the enzyme. The stimulation with endogeneous NO inducers, TNF-α/IFN-γ, also increased ADPRase activity through NO synthesis. Futhermore, ADPRase activity may be mediated by the post-translational modification of ADPRase, ADP-ribosylation. Conclusion: These results indicate that NO synthesized by macrophage activation plays a critical role in the increase in ADPRase activity following ADP-ribose metabolism.
참고문헌 (18)
- "Involvement of cytosolic NAD glycohydrolase in cyclic ADP-ribose metabolism", 253, pp.246~252 (1998)
- "Purification and characterization of NAD glycohydrolase from rabbit erythrocytes", 305, pp.147~152 (1993)
- "Potentiation of calcium- and caffeine-induced calcium release by cyclic ADP-ribose", 268, pp.293~299 (1993)
- "Purification and characterization of adenosine diphosphate ribose pyrophosphatase from human erythrocytes", 30, pp.629~638 (1998)
- "Specific ADP-ribose pyrophosphatase from Artemia cysts and rat liver effects of nitroprusside", pp.121~127 (1996)
- "ADP-ribose pyrophosphatase-I partially purified from livers of rats overdosed with acetaminophen reveals enzyme inhibition in vivo reverted in vitro by dithiothreitol", 13, pp.171~177 (1999)
- "Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB a gene associated with tellurite resistance", pp.32318~32324 (1999)
- "Cloning, expression and characterization of YSA1H, a human adenosine 5'-diphosphosugar pyrophosphatase possessing a MutT motif.", pp.331~337 (1999)
- "Orf186 represents a new member of the Nudix hydrolases active on adenosine", -ade(sine), pp.3192~3197 (1998)
- "Identification and characterization of the Nudix hydrolase from the Archaeon", 273, pp.20924~20928 (1998)
- "Cloning and characterization of a new member of the Nudix hydrolases from human and mouse", pp.8844~8853 (2000)
- "The MutT proteins or "Nudix" hydrolases, a family of versatile, widely distributed, housecleaning" enzymes", pp.25059~25062 (1996)
- "Rat liver mitochondrial ADP-ribose pyrophosphatase in the matrix space with low Km for free ADP-ribose", 299, pp.679~682 (1994)
- "Rat liver nucleoside diphosphosugar or diphosphoalcohol pyrophosphatases different from nucleotide pyrophosphatase or phosphodiesterase I substrate specificities of Mg - dependent hydrolases acting on ADP-ribose", pp.167~177 (1995)
- "a member of the Nudix hydrolase family is an evolutionarily conserved mitochondrial ADP-ribose pyrophosphatase", pp.1794~1801 (2003)
- "Inhibition and ADP-ribose pyrophosphatase-I by nitric-oxide-generating systems:a mechanism linking nitric oxide to processes dependent on free ADP-ribose", 213, pp.1075~1081 (1995)
- "A novel fluorometric assay for ADP-ribose pyrophosphatase activity", 63, pp.161~169 (2005)
- "A self-restricted CD38-connexin 43 cross-talk affects NAD and cyclic ADP-ribose metabolism and regulates intracellular calcium in 3T3 fibroblasts", 276, pp.48300~48308 (2001)