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In vitro Peroxynitrite Scavenging Activity of Arctic Seaweed Extracts

서영완|Hee-Jung Lee|You Ah Kim|Jung-Im Lee|Ji Hee Kim|Hosung Chung|Sung-Ho Kang
한국해양과학기술원 (Korea Institute of Ocean Science & Technology)|2007.06|Ocean and Polar Research|제29권 제2호|pp. 187-191

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참고문헌

주제분류해양학

초록

Abstract

In this study, antioxidant activities of acetone/dichloromethane and methanol extracts of Arctic seaweeds were investigated. The antioxidant properties of both extracts of arctic seaweed were evaluated using two different peroxynitrite tests, including scavenging power on authentic peroxynitrite and inhibitory activities on peroxynitrite generation from 3-morpholinosydnonimine (SIN-1) producing superoxide anion and nitric oxide simultaneously in vitro. At concentration of 10 mg/ml, the acetone/dichloromethane and methanol extracts of Odonthalia dentata exhibited 54.6 and 64.2% inhibition against peroxynitrite generation from SIN-1 while they exhibited 24.3 and 23.0% scavenging activities on authentic peroxynitrite, respectively. On the other hand, the acetone/dichloromethane extract of Polysiphonia stricta exhibited 61.8% inhibition only against the induced peroxynitrite from SIN-1. Furthermore, the crude extracts of Odonthalia dentata and Polysiphonia stricta were fractionated into n-hexane, 85% aq. MeOH, n-BuOH, and H2O fractions, successively, and only 85% aq. MeOH fraction exhibited the best inhibition.

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