신체 부위별 표피에서 밀착연접 단백질 중 오클루딘의 발현도 연구
The Expression Pattern of the Tight Junction Protein Occludin in the Epidermal Context When Comparing Various Physical Samples
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
‘Tight junctions (TJ)’ have recently been identified in the granular cell layer of the human epidermis, where they contribute to the normal adhesion between keratinocytes and to the physiologic barrier function of the epidermis. Among the TJ proteins in the epidermis, occludin is an important transmembrane protein, which is considered as a major component. The purpose of this study is to investigate whether regional variation exists in the expression of the tight junction protein occludin in normal human epidermis. Indirect immunofluorescence staining for occludin was performed with specimens taken from different areas of normal skin (4 from each of 7 different anatomical sites, including the scalp, face, posterior neck, upper arm, abdomen, lower back, and inner thigh). The degrees of the expression-intensity in each specimen were estimated with the reciprocals of positive end-point titer of occludin in an indirect immunofluorescence study. The highest degree expression-intensity of the TJ protein occludin among the different areas of normal epidermis was observed on the face and abdomen with a titer of 600 (p=0.001). The lowest intensity of expression of occludin was seen in the epidermis from the upper arm. Skin specimens from the scalp, neck, back, and leg demonstrated intermediate degrees of the expression in intensity. The expression of occludin in the skin samples obtained from different locations of the body showed a statistically significant variation. This suggests that there is a certain degree of regional variation in the expression-intensity of TJ protein ‘occludin’ in the human epidermis.
Abstract
‘Tight junctions (TJ)’ have recently been identified in the granular cell layer of the human epidermis, where they contribute to the normal adhesion between keratinocytes and to the physiologic barrier function of the epidermis. Among the TJ proteins in the epidermis, occludin is an important transmembrane protein, which is considered as a major component. The purpose of this study is to investigate whether regional variation exists in the expression of the tight junction protein occludin in normal human epidermis. Indirect immunofluorescence staining for occludin was performed with specimens taken from different areas of normal skin (4 from each of 7 different anatomical sites, including the scalp, face, posterior neck, upper arm, abdomen, lower back, and inner thigh). The degrees of the expression-intensity in each specimen were estimated with the reciprocals of positive end-point titer of occludin in an indirect immunofluorescence study. The highest degree expression-intensity of the TJ protein occludin among the different areas of normal epidermis was observed on the face and abdomen with a titer of 600 (p=0.001). The lowest intensity of expression of occludin was seen in the epidermis from the upper arm. Skin specimens from the scalp, neck, back, and leg demonstrated intermediate degrees of the expression in intensity. The expression of occludin in the skin samples obtained from different locations of the body showed a statistically significant variation. This suggests that there is a certain degree of regional variation in the expression-intensity of TJ protein ‘occludin’ in the human epidermis.
참고문헌 (29)
- Balda MS, "Transmembrane proteins of tight junctions", Semin Cell DevBiol, 11, pp.281~289 (2000)
- Brandner JM, "Organization and formation of the tight junction system in human epidermis and cultured keratinocytes", Eur J Cell Biol, 81, pp.253~263 (2002)
- Brandner JM, "Expression and localization of tight junction-associated proteins in human hair follicles", Arch Dermatol Res, 295, pp.211~221 (2003)
- Conklin BS, "Shear stress regulates occludin and VEGF expression in porcine arterial endothelial cells", J Surg Res, 102, pp.13~21 (2002)
- Feldman GJ, "Occludin: Structure, function and regulation", Adv Drug Deliv Rev, 57, pp.883~917 (2005)
- Fluhr JW, "Impact of anatomical location on barrier recovery, surface pH and stratum corneum hydration after acute barrier disruption", Br J Dermatol, 146, pp.770~776 (2002)
- Furuse M, "Claudin-based tight junctions are crucial for the mammalian epidermal barrier", J Cell Biol, 156, pp.1099~1111 (2002)
- Gonzalez-Mariscal L, "Tight junction proteins", Prog Biophys Mol Biol, 81, pp.1~44 (2003)
- Gonzalez-Mariscal L, "Tight junction proteins ZO-1, ZO-2, and occludin along isolated renal tubules", Kidney Int, 57, pp.2386~2402 (2000)
- Green KJ, "Are desmosomes more than tethers for intermediate filaments?", Nat Rev Mol Cell Biol, 1, pp.208~216 (2000)
- Gumbiner BM, "Cell adhesion: The molecular basis of tissue architecture and morphogenesis", Cell, 84, pp.345~357 (1996)
- Harhaj NS, "Regulation of tight junctions and loss of barrier function in pathophysiology", Int J Biochem Cell Biol, 36, pp.1206~1237 (2004)
- Hirase T, "Occludin: A novel integral membrane protein localizing at tight junctions", J Cell Biol, 123, pp.1777~1788 (1993)
- Hirase T, "Occludin as a possible determinant of tight junction permeability in endothelial cells", J Cell Sci, 110, pp.1603~1613
- Huzaira M, "Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy", J Invest Dermatol, 116, pp.846~852 (2001)
- Lapierre LA., "The molecular structure of the tight junction", Adv Drug Deliv Rev, 41, pp.255~264 (2000)
- Malminen M, "Immunohistological distribution of the tight junction components ZO-1 and occludin in regenerating human epidermis", Br J Dermatol, 149, pp.255~260 (2003)
- Matter K, "Mammalian tight junctions in ther regulation of epithelial differentiation and proliferation", Curr Opin Cell Biol, 17, pp.453~458 (2005)
- McGrath JA, "Rook's textbook of dermatology", pp.8~12 (2004), Blackwell publishing
- Morita K, "Tight junctions in the skin", J Dermatol Sci, 31, pp.81~89 (2003)
- Ohnemus U, "Regulation of epidermal tight-junctions during infection with exfoliativ toxin-negative Staphylococcus strains", J Invest Dermatol In press, 128(4), pp.906~916 (2008)
- Pummi K, "Epidermal tight junctions: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes", J Invest Dermatol, 117, pp.1050~1058 (2001)
- Saitou M, "Mammalian occludin in epithelial cells: its expression and subcellular distribution", Eur J Cell Biol, 73, pp.222~231 (1997)
- Schlüter H, "Sealing the live part of the skin: The integrated meshwork of desmosomes, tight junctions and curvilinear ridge structures in the cells of the uppermost granular layer of the human epidermis", Eur J Cell Biol, 83, pp.655~665 (2004)
- Schneeberger EE, "The tight junction: a multifunctional complex", Am J physiol Cell Physiol, 286, pp.1213~1228 (2004)
- Segre JA, "Epidermal barrier formation and recovery in skin disorders", J Clin Invest, 116, pp.1150~1158 (2006)
- Van Itallie CM, "Occludin confers adhesiveness when expressed in fibroblasts", J Cell Sci, 110, pp.1113~1121 (1997)
- Wan H, "Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions", J Clin Invest, 104, pp.123~133 (1999)
- Yoshida Y, "Altered expression of occludin and tight junction formation in psoriasis", Arch Dermatol, 293, pp.239~244 (2001)