건성안 환자의 눈물 내 케모카인 리간드 5의 농도 분석
Analysis of CCL5 Concentration in Tears of Dry Eye Patients
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목적: 건성안 환자의 눈물 내의 RANTES의 농도에 대해 분석하고자 하였다. 대상과 방법: 건성안 환자 40명(쇼그렌증후군 15명, 비쇼그렌증후군 25명)과 정상대조군 10명을 대상으로 눈물 내의 RANTES의 농도를효소면역요법으로 측정하였고, 건성안 환자의 RANTES 농도와 눈물막파괴시간, 기저눈물분비, 눈물청소율, 각막상피병증, 그리고 결막의 술잔세포밀도를 포함한 눈물막 및 안구표면인자와의 상관성에 대해 조사하였다. 결과: 눈물 내 RANTES의 농도는 쇼그렌 증후군 환자에서 435.46 ± 104.45 pg/ml, 비쇼그렌증후군 건성안 환자에서 257.42 ± 46.72pg/ml, 대조군에서 97.53 ± 29.15 pg/ml로 각 군 간 유의한 차이를 보였다(p＜0.01). 건성안 환자에서 RANTES의 농도는 기저눈물분비, 눈물청소율, 각막상피병증, 그리고 결막술잔세포의 밀도와 유의한 상관관계를 보였다(p＜0.05). 결론: 건성안 환자의 눈물에서 RANTES의 농도가 증가하였으며, 이는 여러 눈물막 및 안구표면인자와 상관성이 있고 질병의 중등도와관련이 있었다.
Abstract
Purpose: To investigate the expression of CCL5/RANTES (regulated upon activation, normal T cell expressed and secreted) in the tears of dry eye patients. Methods: Forty patients with dry eye (15 Sjögren’s and 25 non-Sjögren’s syndrome patients) and ten control subjects were recruited for the present study. The concentration of RANTES in tears was measured using an enzyme-linked immunosorbent assay. The correlations between RANTES level, tear film and ocular surface parameters, including tear film break-up time, basal tear secretion, tear clearance rate, corneal sensation, keratoepitheliopathy, and conjunctival goblet cell density, were analyzed in patients with dry eye syndrome. Results: The concentrations of RANTES were 435.46 ± 104.45 pg/ml in Sjögren’s syndrome patients, 257.42 ± 46.72pg/ml in non-Sjögren’s syndrome patients, and 97.53 ± 29.15 pg/ml in the control patients (p < 0.01). The levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy, and goblet cell density (p < 0.05). Conclusions: CCL5/RANTES level increases in the tears of dry eye patients and correlates with various tear film and ocular surface parameters.
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