사물탕투여가 임신랫드의 모체 및 태자에 미치는 영향 ― 유해금속(As, Cd, Pb, Hg)과 양-반응관계를 중심으로 ―
Metal Concentrations in Rats and Fetuses Treated by Herbal Decoction, Samultang
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Purpose:To evaluate the effects of Samultang(SMT) on the metals in the dams and fetuses. Method:SMT(Decoction) was administered orally using different concentrations to pregnant Sprague- Dawley rats for 20days after being assigned randomly to 5 groups. Each group has 11 dams. Pregnant rats were sacrificed at the 20th day of gestation and metal concentration in the dams and fetuses was observed using a ICP-MS and mercury analyzer. Results:Hg concentration in the blood of dams was lower in the SMT×1, SMT×2, SMT×4group than VC(Vehicle Control) and Control group. Hg concentration in the organs of dams was lower in the SMT×1, SMT×2, SMT×4 group than VC, Control group. Whereas Cd concentration in the organs of fetuses was getting lower as increasing SMT dose, Hg concentration in the organs of fetuses was getting higher as increasing SMT dose. Discussion and Conclusion:There were not any significant harmful differences of metal concentration in the dams and fetuses treated by SMT. Particularly, SMT showed the possibility of efficacy to reduce the concentration of Hg in dams but to increase the concentration of Hg in fetuses. The mechanism of reduction is not yet proven. A hypothesis says there is a possibility of the chelation effect between the essential elements of herbs and heavy metals. Another hypothesis is the possibility of Hg transmission from dam to fetus. Further study should be conducted to make guidelines for the safe use of herbal medicine.
Abstract
Purpose:To evaluate the effects of Samultang(SMT) on the metals in the dams and fetuses. Method:SMT(Decoction) was administered orally using different concentrations to pregnant Sprague- Dawley rats for 20days after being assigned randomly to 5 groups. Each group has 11 dams. Pregnant rats were sacrificed at the 20th day of gestation and metal concentration in the dams and fetuses was observed using a ICP-MS and mercury analyzer. Results:Hg concentration in the blood of dams was lower in the SMT×1, SMT×2, SMT×4group than VC(Vehicle Control) and Control group. Hg concentration in the organs of dams was lower in the SMT×1, SMT×2, SMT×4 group than VC, Control group. Whereas Cd concentration in the organs of fetuses was getting lower as increasing SMT dose, Hg concentration in the organs of fetuses was getting higher as increasing SMT dose. Discussion and Conclusion:There were not any significant harmful differences of metal concentration in the dams and fetuses treated by SMT. Particularly, SMT showed the possibility of efficacy to reduce the concentration of Hg in dams but to increase the concentration of Hg in fetuses. The mechanism of reduction is not yet proven. A hypothesis says there is a possibility of the chelation effect between the essential elements of herbs and heavy metals. Another hypothesis is the possibility of Hg transmission from dam to fetus. Further study should be conducted to make guidelines for the safe use of herbal medicine.
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