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Identification and characterization of S-RNase genes in apple rootstock and the diversity of S-RNases in Malus species

박종인|노일섭|김회택|Shigeki Moriya|Kazuma Okada|Kazuyuki Abe|Toshiya Yamamoto
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2016.03|Journal of Plant Biotechnology|제43권 제1호|pp. 49-57

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참고문헌

키워드

AppleSelf-incompatibilityS-RNaseMalus prunifoliaMarubakaido
주제분류농학

초록

We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock ‘Marubakaido’ (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus × domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus × domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

Abstract

We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock ‘Marubakaido’ (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus × domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus × domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

참고문헌 (47)

  1. Bošković R, "Correlation of stylar ribonuclease isoenzymes with incompatibility alleles in apple", Euphytica, 107, pp.29~43 (1999)
  2. Broothaerts W, "New findings in apple S-genotype analysis resolve previous confusion and request the re-numbering of some S-alleles", Theor Appl Genet, 106, pp.703~714 (2003)
  3. Broothaerts W, "cDNA cloning and molecular analysis of two self-incompatibility alleles from apple", Plant Mol Bio, l27, pp.499~3511 (1995)
  4. Dantas ACDe M, "Germination of pollen and the development of pollen tubes in apple (Malus spp.)", Rev Bras Frutic, 27(3), pp.356~359 (2005)
  5. De Nettancourt D, "Monographs on Theor Appl Genet", pp.28~357 (1977), Springer-Verlag
  6. De Nettancourt D, "Incompatibility and incongruity in wild and cultivated plants" (2001), Springer-Verlag
  7. Dreesen RSG, "Analysis of Malus S-RNase gene diversity based on a comparative study of old and modern apple cultivars and European wild apple", Mol Breed, 26, pp.693~709 (2010)
  8. Fazio G, "Analysis of Malus S-RNase gene diversity based on a comparative study of old and modern apple cultivars and European wild apple", Mol Breed, 26, pp.693~709 (2010)
  9. Gu C, "Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species", Can J Plant Sci, 95(2), pp.213~225 (2015)
  10. Heng W, "Recognition specificity of self-incompatibility in Pyrus and Malus", Mol Breed, 28, pp.549~557 (2011)
  11. Inomata Y, "Growth and fruit productivity habits of columnar type apple selections", Horticultural Research, 3(4), pp.392~340 (2004)
  12. Ishimizu T, "Identification of regions in which positive selection may operate in S-RNase of Rosaceae: Implication for S-allele-specific recognition sites in S-RNase", FEBS Letters, 440, pp.337~342 (1998)
  13. Ishimizu T, "Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility", Plant Mol Biol, 37, pp.931~941 (1998)
  14. Janick J, "Fruit Breeding, Tree and Tropical Fruits. Vol.2", pp.1~77 (1996), Wiley
  15. Janssens GA, "A molecular method for S-allele identification in apple based on allele-specific PCR", Theor Appl Genet, 91, pp.691~698 (1995)
  16. Kim H, "Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.)", Mol Breed, 23, pp.463~472 (2009)
  17. 노일섭, "Molecular Characterization of New S-RNases 31 ' and 'S3 2 I in Apple (Malus x domestica Borkh.)", Journal of Plant Biology(한국식물학회지), 51(3), pp.202~208 (2008), 한국식물학회
  18. Hoy Taek Kim, "Determination of Self-Incompatibility Genotypes of Korean Apple Cultivars Based on S-RNase PCR", Journal of Plant Biology(한국식물학회지), 49(6), pp.448~454 (2006), 한국식물학회
  19. Kitahara K, "Sequence of the S10 cDNA from Mclntosh apple and a PCR-digestion identification method", HortScience, 37, pp.187~190 (2002)
  20. Kitahara K, "Cloning of the S25 cDNA from Mclntosh apple and an S25-allele identification method", J Hortic Sci Biotechnol, 77, pp.724~728 (2002)
  21. Kitahara K, "Complete sequences of the S-genes ‘Sd-’ and ‘Sh-RNase’ cDNA in apple", HortScience, 35, pp.712~715 (2000)
  22. Kobel F, "Weitere Untersuchungen uber die Befruchtungsverhaltnisse der Apfel- und Birnsorten", Landw Jb Schweiz, 53, pp.160~191 (1939)
  23. Komori S, "Analyzes of S-allele genotypes of ‘McIntosh’, ‘Kitakami’, ‘Worcester Pearmain’, etc", J Jpn Soc Hortic Sci, 68(suppl. 2), pp.94~ (1999)
  24. Komori S, "Analysis of S-allele genotypes and genetic diversity in the apple", Acta Hortic, 538, pp.83~86 (2000)
  25. Korban SS, "Nomenclature of the cultivate apple", HortScience, 19, pp.177~180 (1984)
  26. Larsen B, "A high-throughput method for genotyping S-RNase alleles in apple", Mol Breeding, 36, pp.24~ (2016)
  27. Long S, "Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars", Tree Genet Genomes, 6, pp.161~168 (2010)
  28. Matityahu A, "Molecular identification of a new apple S-RNase-S29-cloned from ‘Anna’, a low-chilling-requirement cultivar", HortScience, 40, pp.850~851 (2005)
  29. "Database of S-RNase" (2013)[URL]
  30. Matsumoto S, "Apple pollination biology for stable and novel fruit production: search system for apple cultivar combination showing incompatibility, semicompatibility, and full-compatibility based on the S-RNase allele database", Int J Agron, 2014, pp.1~9 (2014)
  31. Matsumoto S, "Genomic DNA sequence of S16c(=16)-RNase in apple: re-numbering of S16(=27a) - and S22(=27b)-allele to S16a and S16b", Sci Rep Rac Educ Gifu Univ (Nat. Sci.), 29, pp.7~12 (2005)
  32. Matsumoto S, "Partial genomic sequences of S6 -, S12 -, S13 -, S14 -, S17-, S19 -, and S21 -RNases of apple and their allele designations", Plant Biotechnol, 20, pp.323~329 (2003)
  33. Matsumoto S, "Genomic DNA sequence encoding Malus × domestica Borkh. “Akane”, “Delicious” and Malus transitoria S-RNases", DNA Seq, 12, pp.381~383 (2001)
  34. Matsumoto S, "Genomic DNA sequence encoding Malus × domestica Borkh. “Akane”, “Delicious” and Malus transitoria S-RNases", DNA Seq, 12, pp.381~383 (2001)
  35. Matsumoto S, "Discovery of a new self-incompatibility allele in apple", HortScience, 35, pp.1329~1332 (2000)
  36. Matsumoto S, "A new S-allele in apple, ‘Sg’, and its similarity to the ‘Sf’ allele from Fuji", HortScience, 34, pp.708~710 (1999)
  37. Matsumoto S, "S-genotypes of 15 apple cultivars and self-compatibility of ‘Megumi’", J Jpn Soc Hortic Sci, 68, pp.236~241 (1999)
  38. Matsumoto S, "Possible involvement of a new S-gene ‘St-RNase’ (Accession No. AB035928) in the wild apple possessing high similarity to the ‘S3-’and ‘S5-RNase’ in the Japanese pear", Plant Physiol, 122, pp.620~ (2000)
  39. Matsumoto S, "Structural and functional analyses of two new S-RNase alleles, Ssi5 and Sad5, in apple", J Hortic Sci Biotechnol, 85, pp.131~136 (2010)
  40. Sassa H, "Identification of self-incompatibility related glycoproteins in styles of apple (Malus × domestica)", Theor Appl Genet, 89, pp.201~205 (1994)
  41. Sassa H, "Self-incompatibility (S) alleles of the Rosaceae encode members of a distinct class of the T2", S ribonuclease superfamily, 1996, pp.250, 547~557
  42. Schneider D, "Analysis of S-alleles by PCR for determination of compatibility in the Red Delicious apple orchard", J Hortic Sci Biotechnol, 76, pp.596~600 (2001)
  43. Ushijima K, "Cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis): primary structural features and sequence diversity of the S-RNases in Rosaceae", Mol Genet Gen, 260, pp.261~268 (1998)
  44. van Nerum I, "Re-examination of the self-incompatibility genotype of apple cultivars containing putative ‘new’ S alleles", Theor Appl Genet, 103, pp.584~591 (2001)
  45. Verdoodt L, "Use of the multi-allelic self-incompatibility gene in apple to assess homozygosity in shoots obtained through haploid induction", Theor Appl Genet, 96, pp.294~300 (1998)
  46. Vieira J, "Different positively selected sites at the gametophytic self-incompatibility pistil S-RNase gene in the Solanaceae and Rosaceae (Prunus, Pyrus, and Malus)", J Mol Evol, 65, pp.175~185 (2007)
  47. Yamamoto T, "DNA profiling of fresh and processed fruits in pear", Breed Sci, 56, pp.165~171 (2006)

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