Transcriptional Response of Pectobacterium carotovorum to Cinnamaldehyde Treatment
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown gall and bacterial canker diseases, respectively has been documented, indepth studies into cinnamaldehyde’s broader influence on plant pathogenic bacteria are relatively unexplored. Particularly, Pectobacterium spp., gram-negative soil-borne pathogens, notoriously cause soft rot damage across a spectrum of plant families, emphasizing the urgency for effective treatments. Our investigation established that the Minimum Inhibitory Concentrations (MICs) of cinnamaldehyde against strains P. odoriferum JK2, P. carotovorum BP201601, and P. versatile MYP201603 were 250 μg/ml, 125 μg/ml, and 125 μg/ml, respectively. Concurrently, their Minimum Bactericidal Concentrations (MBCs) were found to be 500 μg/ml, 250 μg/ml, and 500 μg/ml, respectively. Using RNA-sequencing analysis, we identified 1,907 differentially expressed genes in P. carotovorum BP201601 treated with 500 μg/ml cinnamaldehyde. Notably, our results indicate that cinnamaldehyde upregulated nitrate reductase pathways while downregulating the citrate cycle, suggesting a potential disruption in the aerobic respiration system of P. carotovorum during cinnamaldehyde exposure. This study serves as a pioneering exploration of the transcriptional response of P. carotovorum to cinnamaldehyde, providing insights into the bactericidal mechanisms employed by cinnamaldehyde against this bacterium.
Abstract
Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown gall and bacterial canker diseases, respectively has been documented, indepth studies into cinnamaldehyde’s broader influence on plant pathogenic bacteria are relatively unexplored. Particularly, Pectobacterium spp., gram-negative soil-borne pathogens, notoriously cause soft rot damage across a spectrum of plant families, emphasizing the urgency for effective treatments. Our investigation established that the Minimum Inhibitory Concentrations (MICs) of cinnamaldehyde against strains P. odoriferum JK2, P. carotovorum BP201601, and P. versatile MYP201603 were 250 μg/ml, 125 μg/ml, and 125 μg/ml, respectively. Concurrently, their Minimum Bactericidal Concentrations (MBCs) were found to be 500 μg/ml, 250 μg/ml, and 500 μg/ml, respectively. Using RNA-sequencing analysis, we identified 1,907 differentially expressed genes in P. carotovorum BP201601 treated with 500 μg/ml cinnamaldehyde. Notably, our results indicate that cinnamaldehyde upregulated nitrate reductase pathways while downregulating the citrate cycle, suggesting a potential disruption in the aerobic respiration system of P. carotovorum during cinnamaldehyde exposure. This study serves as a pioneering exploration of the transcriptional response of P. carotovorum to cinnamaldehyde, providing insights into the bactericidal mechanisms employed by cinnamaldehyde against this bacterium.
참고문헌 (45)
- He Z, "Antimicrobial activity of cinnamaldehyde on Streptococcus mutans biofilms", Front. Microbiol, 10, pp.2241~ (2019)
- Kim ME, "Anti-inflammatory effects of trans-cinnamaldehyde on lipopolysaccharide-stimulated macrophage activation via MAPKs pathway regulation", Immunopharmacol. Immunotoxicol, 40, pp.219~224 (2018)
- Zhu R, "Cinnamaldehyde in diabetes : A review of pharmacology, pharmacokinetics and safety", Pharmacol. Res, 122, pp.78~89 (2017)
- Akshaya BS, "Cinnamaldehyde inhibits Enterococcus faecalis biofilm formation and promotes clearance of its colonization by modulation of phagocytes in vitro", Microb. Pathog, 181, pp.106157~ (2023)
- Buglak NE, "Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats", Redox Biol, 19, pp.166~178 (2018)
- Wang Y, "Cinnamaldehyde inhibits the growth of Phytophthora capsici through disturbing metabolic homoeostasis", PeerJ, 9, pp.e11339~ (2021)
- Qu S, "Cinnamaldehyde, a promising natural preservative against Aspergillus flavus", Front. Microbiol, 10, pp.2895~ (2019)
- Duan B, "Cinnamaldehyde promotes the defense response in postharvest citrus fruit inoculated with Penicillium digitatum and Geotrichum citri-aurantii", Pestic. Biochem. Physiol, 179, pp.104976~ (2021)
- Zhao W, "First report on Colletotrichum fructicola causing anthracnose in Chinese sorghum and its management using phytochemicals", J. Fungi, 9, pp.279~ (2023)
- Guo H, "Biocontrol of gray mold of cherry tomatoes with the volatile organic monomer from Hanseniaspora uvarum, Trans-Cinnamaldehyde", Food Bioproc. Tech, 12 (2019)
- Wei J, "Antifungal activity of cinnamaldehyde against Fusarium sambucinum involves inhibition of ergosterol biosynthesis", J. Appl. Microbiol, 129, pp.256~265 (2020)
- Ahmed B, "Inhibition of growth, biofilm formation, virulence, and surface attachment of Agrobacterium tumefaciens by cinnamaldehyde derivatives", Front. Microbiol, 13, pp.1001865~ (2022)
- Song YR, "Antibacterial activity of cinnamaldehyde and estragole extracted from plant essential oils against Pseudomonas syringae pv. actinidiae causing bacterial canker disease in kiwifruit", Plant Pathol J, 32, pp.363~370 (2016)
- Zaczek-Moczydłowska MA, "Pectobacterium and Dickeya species detected in vegetables in Northern Ireland", Eur. J. Plant Pathol, 154, pp.635~647 (2019)
- Kang M, "Inactivation of Pectobacterium carotovorum subsp. carotovorum on Chinese cabbage (Brassica rapa L. subsp. pekinensis) by wash treatments with phenolic compounds", Food Sci. Technol, 93, pp.229~236 (2018)
- Chandrashekar BS, "Host range and virulence diversity of Pectobacterium carotovorum subsp. brasiliense strain RDKLR infecting radish in India, and development of a LAMPbased diagnostics", J. Appl. Microbiol, 132, pp.4400~4412 (2022)
- Youdkes D, "Potential control of potato soft rot disease by the obligate predators bdellovibrio and like organisms", Appl. Environ. Microbiol, 86, pp.e02543-19~ (2020)
- Performance standards for antimicrobial susceptibility testing: 20th informational supplement, "Wayne P", Clinical and laboratory standards institute, ~, pp.CLSI document M100-S20 (2010)
- Sader HS, "Potency and bactericidal activity of iclaprim against recent clinical gram-positive isolates", Antimicrob Agents Chemother, 53, pp.2171~2175 (2009)
- Capra EJ, "Evolution of two-component signal transduction systems", Annu. Rev. Microbiol, 66, pp.325~347 (2012)
- Vanzi F, "Protein synthesis by single ribosomes", RNA, 9, pp.1174~1179 (2003)
- Garmory HS, "ATP-binding cassette transporters are targets for the development of antibacterial vaccines and therapies", Infect. Immun, 72, pp.6757~6763 (2004)
- Wilson DF, "Oxidative phosphorylation : regulation and role in cellular and tissue metabolism", J. Physiol, 595, pp.7023~7038 (2017)
- Wood NJ, "Maximal expression of membrane-bound nitrate reductase in Paracoccus is induced by nitrate via a third FNR-like regulator named NarR", J. Bacteriol, 183, pp.3606~3613 (2001)
- Maklashina E, "Anaerobic expression of Escherichia coli succinate dehydrogenase : functional replacement of fumarate reductase in the respiratory chain during anaerobic growth", J. Bacteriol, 180, pp.5989~5996 (1998)
- Buck D, "Cloning and expression of the succinyl-coA synthetase genes of Escherichia coli K12", Microbiol, 132, pp.1753~1762 (1986)
- Marino M, "Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM(ywiD)", J. Bacteriol, 183, pp.6815~6821 (2001)
- Zhang Q, "The metabolic adaptation in response to nitrate is critical for Actinobacillus pleuropneumoniae growth and pathogenicity under the regulation of NarQ", P, 2022, pp.90, e00239-00222~
- Lopez CA, "Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration", Science, 353, pp.1249~1253 (2016)
- Snoep JL, "The role of lipoic acid in product formation by Enterococcus faecalis NCTC 775 and reconstitution in vivo and in vitro of the pyruvate dehydrogenase complex", J. Gen. Microbiol, 139(Pt 6), pp.1325~1329 (1993)
- Gill AO, "Mechanisms of bactericidal action of cinnamaldehyde against Listeria monocytogenes and of eugenol against L. monocytogenes and Lactobacillus sakei", Appl. Environ. Microbiol, 70, pp.5750~5755 (2004)
- Verdon G, "Crystal Structures of the ATPase subunit of the glucose ABC transporter from sulfolobus solfataricus : Nucleotide-free and nucleotide-bound conformations", J. Mol. Biol, 330, pp.343~358 (2003)
- Wang Y, "Regulation of membrane permeability by a two-component regulatory system in Pseudomonas aeruginosa", Antimicrob. Agents Chemother, 47, pp.95~101 (2003)
- Topa SH, "Activity of cinnamaldehyde on quorum sensing and biofilm susceptibility to antibiotics in Pseudomonas aeruginosa", Microorganisms, 8, pp.455~ (2020)
- Li T, "Inhibition of quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas fluorescens by cinnamaldehyde", Int. J. Food Microbiol, 269, pp.98~106 (2018)
- Li S, "Cinnamaldehyde decreases the pathogenesis of Aeromonas hydrophila by inhibiting quorum sensing and biofilm formation", Fishes, 8, pp.122~ (2023)
- Ahmed B, "Inhibition of growth, biofilm formation, virulence, and surface attachment of Agrobacterium tumefaciens by cinnamaldehyde derivatives", Front. Microbiol, 13, pp.1001865~ (2022)
- Pereira WA, "New Insights into the antimicrobial action of cinnamaldehyde towards Escherichia coli and its effects on intestinal colonization of mice", Biomolecules, 11, pp.302~ (2021)
- Mousavi F, "Cinnamaldehyde characterization as an antibacterial agent toward E. coli metabolic profile using 96-blade solid-phase microextraction coupled to liquid chromatography–mass spectrometry", J. Proteome Res, 15, pp.963~975 (2016)
- Kapoor G, "Action and resistance mechanisms of antibiotics: A guide for clinicians", J. Anaesthesiol. Clin0Pharmacol, 33, pp.300~305 (2017)
- Epand RM, "Molecular mechanisms of membrane targeting antibiotics", Biochim. Biophys. Acta-Biomembr, 1858, pp.980~987 (2016)
- Nikaido H, "Multidrug resistance in bacteria", Annu. Rev. Biochem, 78, pp.119~146 (2009)
- Czajkowski R, "Control of blackleg and tuber soft rot of potato caused by Pectobacterium and dickeya species : a review", Plant Pathol, 60, pp.999~1013 (2011)
- Vu NT, "Antibiotic resistance of Pectobacterium Korean strains susceptible to the bacteriophage phiPccP-1", Res. Plant Dis, 28, pp.166~171 (2022)
- Usai F, "trans-cinnamaldehyde as a novel candidate to overcome bacterial resistance : An overview of in vitro studies", Antibiotics(Basel), 12, pp.254~ (2023)