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Effect of Sonicated Extracts of Enterococcus faecalis on the Production of Matrix Metalloproteinase-8 by Human Polymorphonuclear Neutrophils

Effect of sonicated extracts of Enterococcus faecalis on the production of matrix metalloproteinase-8 by human polymorphonuclear neutrophils

손원준|박성경|임성삼
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2005.03|Restorative Dentistry and Endodontics|제30권 제2호|pp. 138-144

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참고문헌

주제분류치의학

초록

Enterococcus faecalis 의 추출물을 성인의 말초혈액으로부터 얻은 인간의 백혈구를 이용해 Matrix Metalloproteinase-8 (MMP-8) 의 생산에 미치는 영향에 대해서 알아보았다. Enterococcus faecalis를 배양한 뒤 초음파 분쇄를 하여 추출물을 얻어냈다. 이중 일부는 적정한 농도의 Ca(OH)2를 처리하였다. 이후 E. faecalis 추출물군, E. faecalis 추출물에 Ca(OH)2 를 처리한 군으로 분리하여 다형핵백혈구를 자극하여 MMP-8의 생산량을 측정하였다. MMP-8 는 처리하지 않은 PMN group에서도 나타났고, PMN을 E. coli LPS로 자극시킨 경우 증가하였다 (p<0.05). SEF group 들에서는 MMP-8 의 생성량이 SEF 의 농도가 증가함에 따라 감소하였다 (p<0.05). 따라서 Enterococcus faecalis 는 PMN 의 MMP-8 생산 억제 효과를 가진다고 볼 수 있다. Ca(OH)2로 처리한 SEF 군의 경우 각기 다른 SEF 농도에서도 모두 MMP-8 생성량이 아무 처리도 하지 않은 PMN 군보다 많았다 (p<0.05). 하지만 각 군들 사이에 통계적 차이는 없었다 (p>0.05).

Abstract

This in vitro study monitored MMP-8 production on PMN by stimulated with the following three groups; Sonicated extracts of E. faecalis (SEF), SEF treated with Ca(OH)2 (12.5mg/ml) for 7 days, and lipopolysaccharides (LPS) of E. coli.. The level of MMP-8 in each group was immediately measured by ELISA. The data were analyzed with Kruskal-Wallis test and Mann-Whitney U test.In the SEF group, the level of production of MMP-8 was higher than the negative control group in low concentration (0.05g/ml) of SEF (p<0.05), but it decreased with an increase in the concentration of SEF (p<0.05). In the case of SEF treated with Ca(OH)2, all of the MMP levels were higher than negative control group (p<0.05), but no statistical difference was found among the different SEF concentrations (p>0.05). All of the levels in E. coli LPS were increased with increasing concentrations (p<0.05).According to this study we could summarize as follows: 1. MMP-8 was expressed at low level in untreated PMN group and the levels of MMP-8 were upregulated in PMN stimulated by E. coli LPS groups. 2. In the SEF groups, the level of production of MMP-8 decreased with an increase in the concentration of SEF (p<0.05). So E. faecalis may have suppressive effect on the production of MMP-8 by PMN.3. In the case of SEF treated with Ca(OH)2, all of the MMP levels at different SEF concentrations were higher than untreated PMN group (p<0.05), but no statistical difference was found among the different SEF concentrations (p>0.05).

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