Gender determination in parrots from Korean zoos using chromo-helicase-DNA binding protein 1 (CHD1) gene fragments
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Many parrots are considered endangered species due to threats from human activities. Gender determination is of great importance for biological studies and the conservation of endangered parrots. However, like other birds, gender determination in parrots is hindered due to the lack of external dimorphism between males and females. A molecular approach using the chromo-helicase-DNA binding protein 1 (CHD1) gene is commonly used for sexing birds. This study aimed to determine the gender of parrots from Korean zoos based on amplification and visualization of the partial CHD1 gene. The samples of 13 parrot species were collected from three different zoos in Korea and the extracted DNA templates were amplified using CHD1 gene primers. The gender of 27 samples of 13 species was determined by visualizing the PCR products on an agarose gel. While male parrots were indicated by a single band, female parrots were indicated by double bands. The findings provide additional information, which might be helpful for the management and care of parrots in Korean zoos.
Abstract
Many parrots are considered endangered species due to threats from human activities. Gender determination is of great importance for biological studies and the conservation of endangered parrots. However, like other birds, gender determination in parrots is hindered due to the lack of external dimorphism between males and females. A molecular approach using the chromo-helicase-DNA binding protein 1 (CHD1) gene is commonly used for sexing birds. This study aimed to determine the gender of parrots from Korean zoos based on amplification and visualization of the partial CHD1 gene. The samples of 13 parrot species were collected from three different zoos in Korea and the extracted DNA templates were amplified using CHD1 gene primers. The gender of 27 samples of 13 species was determined by visualizing the PCR products on an agarose gel. While male parrots were indicated by a single band, female parrots were indicated by double bands. The findings provide additional information, which might be helpful for the management and care of parrots in Korean zoos.
참고문헌 (12)
- Bush ER, "Global trade in exotic pets 2006-2012", Conserv. Biol., 28, pp.663~676 (2014)
- Çakmak E, "Comparison of three different primer sets for sexing birds", J. Vet. Diagn. Invest, 29, pp.59~63 (2017)
- Ellegren H, "First gene on the avian W chromosome (CHD)provides a tag for universal sexing of non-ratite birds", Proc. R. Soc. Lond. B-Biol. Sci., 263, pp.1635~1644 (1996)
- Griffiths R, "A DNA test to sex most birds", Mol. Ecol., 7, pp.1071~1075 (1998)
- Griffiths R, "Sex of the last wild Spix’s macaw", Nature, 375, pp.454~ (1995)
- Ito H, "Sex identification by alternative polymerase chain reaction methods in Falconiformes", Zool. Sci., 20, pp.339~344 (2003)
- Jensen T, "Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers", Zoo Biol., 22, pp.561~571 (2003)
- Kahn NW, "Chromosome-specific intron size differences in the avian CHD gene provide an efficient method for sex dentification in birds", The Auk, 115, pp.1074~1078 (1998)
- Lee J, "A novel strategy for avian species and gender identification using the CHD gene", Mol. Cell. Probes, 24, pp.27~31 (2010)
- Miyaki CY, "Sex identification of parrots, toucans, and curassows by PCR: perspectives for wild and captive population studies", Zoo Biol., 17, pp.415~423 (1998)
- Valadan R, "Avian gametologs as molecular tags for sex identification in birds of prey of Iran", Zoo Biol, 36, pp.289~293 (2017)
- Vucicevic M, "Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing", Zoo Biol, 32, pp.269~276 (2013)