Effects of Gelidium amansii extracts on in vitro ruminal fermentation characteristics, methanogenesis, and microbial populations
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objective: Gelidium amansii (Lamouroux) is a red alga belonging to the family Gelidaceae and is commonly found in the shallow coasts of many East Asian countries, including Korea, China, and Japan. G. amansii has traditionally been utilized as an edible alga, and has various biological activities. The objective of this study was to determine whether dietary supplementation of G. amansii could be useful for improving ruminal fermentation. Methods: As assessed by in vitro fermentation parameters such as pH, total gas, volatile fatty acid (VFA) production, gas profile (methane, carbon dioxide, hydrogen, and ammonia), and microbial growth rate was compared to a basal diet with timothy hay. Cannulated Holstein cows were used as rumen fluid donors and 15 mL rumen fluid: buffer (1:2) was incubated for up to 72 h with four treatments with three replicates. The treatments were: control (timothy only), basal diet with 1% G. amansii extract, basal diet with 3% G. amansii extract, and basal diet with 5% G. amansii extract. Results: Overall, the results of our study indicate that G. amansii supplementation is potentially useful for improving ruminant growth performance, via increased total gas and VFA production, but does come with some undesirable effects, such as increasing pH, ammonia concentration, and methane production. In particular, real-time polymerase chain reaction indicated that the methanogenic archaea and Fibrobacter succinogenes populations were significantly reduced, while the Ruminococcus flavefaciens populations were significantly increased at 24 h, when supplemented with G. amansii extracts as compared with controls. Conclusion: More research is required to elucidate what G. amansii supplementation can do to improve growth performance, and its effect on methane production in ruminants.
Abstract
Objective: Gelidium amansii (Lamouroux) is a red alga belonging to the family Gelidaceae and is commonly found in the shallow coasts of many East Asian countries, including Korea, China, and Japan. G. amansii has traditionally been utilized as an edible alga, and has various biological activities. The objective of this study was to determine whether dietary supplementation of G. amansii could be useful for improving ruminal fermentation. Methods: As assessed by in vitro fermentation parameters such as pH, total gas, volatile fatty acid (VFA) production, gas profile (methane, carbon dioxide, hydrogen, and ammonia), and microbial growth rate was compared to a basal diet with timothy hay. Cannulated Holstein cows were used as rumen fluid donors and 15 mL rumen fluid: buffer (1:2) was incubated for up to 72 h with four treatments with three replicates. The treatments were: control (timothy only), basal diet with 1% G. amansii extract, basal diet with 3% G. amansii extract, and basal diet with 5% G. amansii extract. Results: Overall, the results of our study indicate that G. amansii supplementation is potentially useful for improving ruminant growth performance, via increased total gas and VFA production, but does come with some undesirable effects, such as increasing pH, ammonia concentration, and methane production. In particular, real-time polymerase chain reaction indicated that the methanogenic archaea and Fibrobacter succinogenes populations were significantly reduced, while the Ruminococcus flavefaciens populations were significantly increased at 24 h, when supplemented with G. amansii extracts as compared with controls. Conclusion: More research is required to elucidate what G. amansii supplementation can do to improve growth performance, and its effect on methane production in ruminants.
참고문헌 (32)
- Kim KN, "Inhibition of tumor growth in vitro and in vivo by fucoxanthin against melanoma B16F10 cells", Environ Toxicol Pharmacol, 35, pp.39~46 (2013)[DOI]
- 이지혁, "Evaluation of phlorofucofuroeckol-A isolated from Ecklonia cava (Phaeophyta) on anti-lipid peroxidation in vitro and in vivo", ALGAE, 30(4), pp.313~323 (2015), 한국조류학회I[DOI]
- Chowdhury S, "Algae in animal production", Agracultural science of biodiversity and sustainability workshop, pp.3~7 (1995)
- MacArtain P, "Nutritional value of edible seaweeds", Nutr Rev, 65, pp.535~543 (2007)[DOI]
- Moller DE, "Insulin resistance - mechanisms, syndromes, and implications", N Engl J Med, 325, pp.938~948 (1991)[DOI]
- Yan X, "Antioxidative activities in some common seaweeds", Plant Foods Hum Nutr, 52, pp.253~262 (1998)[DOI]
- Pell AN, "Computerized monitoring of gas production to measure forage digestion in vitro", J Dairy Sci, 76, pp.1063~1073 (1993)[DOI]
- Bozic AK, "Effects of the methaneinhibitors nitrate, nitroethane, lauric acid, Lauricidin and the Hawaiian marine algae Chaetoceros on ruminal fermentation in vitro", Bioresour Technol, 100, pp.4017~4025 (2009)[DOI]
- Machado L, "Effects of marine and freshwater macroalgae on in vitro total gas and methane production", PLoS ONE, 9, pp.e85289~ (2014)
- Denman SE, "Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen", FEMS Microbiol Ecol, 58, pp.572~582 (2006)
- Denman SE, "Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane", FEMS Microbiol Ecol, 62, pp.313~322 (2007)[DOI]
- Skillman LC, "Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between Entodinium populations in sheep offered a hay-based diet", Appl Environ Microbiol, 72, pp.200~206 (2006)
- Koike S, "Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens", FEMS Microbiol Ecol, 204, pp.361~366 (2001)[DOI]
- Denman SE, "Quantitative (real-time) PCR;Methods in gut microbial ecology for ruminants", pp.105~115 (2005), Springer
- STAT user's guide: Version 9.2 edn, "SAS", pp.2002, SAS Institute Inc
- McCullough ME, "Sodium acetate and sodium propionate as additives to all-in-one rations for milk production", J Dairy Sci, 52, pp.1605~1608 (1969)[DOI]
- Hiltner P, "Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria", Appl Environ Microbiol, 46, pp.642~648 (1983)
- Ha JK, "Ruminant nutrition and physiology" (2005), Seoul National University press
- Denis C, "Study of the chemical composition of edible red macroalgae Grateloupia turuturu from Brittany (France)", J Food Chem, 119, pp.913~917 (2010)[DOI]
- Machad L, "Effects of marine and freshwater macroalgae on in vitro total gas and methane production", PLoS One, 9, pp.e85289~ (2014)
- 김언태, "Effects of Flavonoid-rich Plant Extracts on In vitro Ruminal Methanogenesis, Microbial Populations and Fermentation Characteristics", Animal Bioscience, 28(4), pp.530~537 (2015), 아세아·태평양축산학회[DOI]
- Latham MJ, "Fermentation of cellulose by Ruminococcus flavefaciens in the presence and absence of Methanobacterium ruminantium", Appl Environ Microbiol, 34, pp.297~301 (1977)
- Finlay BJ, "Some rumen ciliates have endosymbiotic methanogens", FEMS Microbiol Lett, 117, pp.157~161 (1994)[DOI]
- Ntaikou I, "Hydrogen production from sugars and sweet sorghum biomass using Ruminococcus albus", Int J Hydrogen Energy, 33, pp.1153~1163 (2008)[DOI]
- Chaucheyras-Durand F, "Influence of the composition of the cellulolytic flora on the development of hydrogenotrophic microorganisms, hydrogen utilization, and methane production in the rumens of gnotobiotically reared lambs", Appl Environ Microbiol, 76, pp.7931~7937 (2010)[DOI]
- Whitman WB, "The methanogenic bacteria;The Prokaryotes, 2nd ed A handbook on the biology of bacteria", pp.719~767 (1992), Springer-Verlag
- Jouany JP, "Use of 'natural' products as alternatives to antibiotic feed additives in ruminant production", Animal, 1, pp.1443~1466 (2007)
- Archer DB, "Methanogenic bacteria and methane production in various habitats;Anaerobic bacteria in habitats other than man", pp.185~223 (1986)
- Martin C, "Methane mitigation in ruminants: from microbe to the farm scale", Animal, 4, pp.351~365 (2010)[DOI]
- Makoto Mitsumori, "Control of Rumen Microbial Fermentation for Mitigating Methane Emissions from the Rumen", Animal Bioscience, 21(1), pp.144~154 (2008), 아세아·태평양축산학회
- Larsen M, "Effect of abomasal glucose infusion on splanchnic amino acid metabolism in periparturient dairy cows", J Dairy Sci, 92, pp.3306~3318 (2009)[DOI]
- McAllan AB, "Rumen fermentation characteristics and duodenal nutrient flow in lactating dairy cows receiving two types of grass silage with two levels of concentrates", Anim Feed Sci Technol, 46, pp.227~235 (1994)