흰쥐의 섬유아세포 L-929를 이용한 새로운 Soft Contact Lens 소재의 생물안전성 검증
Biosafety of the New Soft Contact Lens Materials in the Fibroblast L-929 Cell Line
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In this study, we polymerized new materials for soft contact lens using HEMA (2-hydroxyethyl methacrylate) which is the based-monomer of soft contact lens, EGDMA (ethylene glycol dimethacrylate) as cross linkage agent, and the new additives of monoester or di-ester derived from itaconic acid commercially produced by the fermentation of Asp. itaconicus. New polymer materials for soft contact lens were synthesized with the mixture of HEMA and mono- or diester at different ratios and presented to a good water content and oxygen transmissibility (Dk/L) values. In case of polymerization with HEMA and mono-ester (15%), the water content and oxygen transmissibility of contact lens were found to be good values at 57.6% and 28.5 Dk respectively. The mixture of HEMA and mono-ester is more excellent than HEMA/di-ester in the water content and oxygen transmissibility. The toxicity of new contact lens materials were confirmed in the fibroblast L-929 cell line using a agar overlay test and a growth inhibition test with the extract solution of contact lens.
Abstract
In this study, we polymerized new materials for soft contact lens using HEMA (2-hydroxyethyl methacrylate) which is the based-monomer of soft contact lens, EGDMA (ethylene glycol dimethacrylate) as cross linkage agent, and the new additives of monoester or di-ester derived from itaconic acid commercially produced by the fermentation of Asp. itaconicus. New polymer materials for soft contact lens were synthesized with the mixture of HEMA and mono- or diester at different ratios and presented to a good water content and oxygen transmissibility (Dk/L) values. In case of polymerization with HEMA and mono-ester (15%), the water content and oxygen transmissibility of contact lens were found to be good values at 57.6% and 28.5 Dk respectively. The mixture of HEMA and mono-ester is more excellent than HEMA/di-ester in the water content and oxygen transmissibility. The toxicity of new contact lens materials were confirmed in the fibroblast L-929 cell line using a agar overlay test and a growth inhibition test with the extract solution of contact lens.
참고문헌 (12)
- Bressler, E, "Conversion of citric acid to itaconic acid in a novel liquid membrane bioreactor", J, Chem. technol. Biotechnol, 75, pp.66~72 (2000)
- Caroline, "Drying methods for XPS analysis of pure VisionTM, Focus Night&DayTM and conventional hydrogel contact lenses", A. Surface Science, 230, pp.106~114 (2004)
- Chung, S. Y, "Production and monitoring of itaconic acid from starchy materials", Korean J. Biotechnol. Bioeing, 17, pp.477~483 (2002)
- IACLE, "The IACLE Contact lens course, Module 2, 5", ~ (1997)
- Kim, J. H, "Comparison of contact lenses and storage solution for cytotoxic potential using a cell growth inhibition assay", J. Korean Oph. Opt. Soc., 4, pp.81~86 (1999)
- Lai, T. C, "Kirk-Othmer Encylopedia of Chemical Technology. 4th ed", John Wiley, 7, pp.192~218 (1993)
- PARK, JUNG-HYUN, "The Effect of Overexpression of Rat Clusterin in L929 Fibroblasts", Journal of Microbiology and Biotechnology, 14(6), pp.1333~1337 (2004), 한국미생물·생명공학회
- P. White, "Contact lens spectrum", 31~44 (1994)
- P. White, "Contact lens Spectrum Suppl.7", ~ (1999)
- Reddy, C. S, "Enhanced production of itaconic acid from corn starch and market refuse fruits by genetically manipulated Aspergillus terreus SKR 10", Bioresour. Tech., 85, pp.69~71 (2002)
- Refojo,M.F, "Polymeric Materials encylopedia", pp.1504~1509 (1996), CRC Press
- Ruben, M, "Contact Lens Practice" (1994), Chapman & Hall Medical