본문 바로가기
KERD
논문KCI 등재

리기테다 소나무 솔방울의 항산화 활성 및 산화적 DNA 손상에 대한 억제 효과

Antioxidant Activity and DNA Protective Effect against Oxidative Stress of Pinus rigida x taeda Cone

장태원|박재호|민영실|최지수|이만효
중소기업융합학회 (The Society of Convergence for Small and Medium Business)|2020.11|융합정보논문지 (Journal of Convergence for Information Technology)|제10권 제11호|pp. 168-176|DOI https://doi.org/10.22156/CS4SMB.2020.10.11.168

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

ABTSDPPH항산화리기테다 소나무 솔방울φX-174 RF I plasmid DNA

초록

활성산소종이 DNA를 손상하고 암을 유발하는데 기인하는 것으로 밝혀지면서 활성산소를 제거하기 위한 항산화 물질 개발 연구가 진행되고 있다. 본 연구에서는 리기테다 소나무 솔방울 에틸아세테이트 분획물의 항산화 효과 및 산화적 스트레스에 의해 야기된 DNA 손상 보호 효과를 조사하기 위해 수행되었다. 항산화 활성을 확인하기 위해 DPPH 및 ABTS 라디칼 소거 활성, 환원력, Fe2+ 킬레이팅 활성을 평가하였으며, 항산화 활성과 연관된 총 페놀 및 비타민 C의 함량도 분석하여 식물 화학물질을 확인하였다. 산화적 DNA 손상 억제 효과는 φX-174 RF I plasmid DNA 절단 분석법을 이용하여 측정하였다. DPPH 및 ABTS 라디칼 소거 활성은 농도 의존적으로 나타났다. 환원력과 Fe2+ 킬레이팅 활성은 200 ㎍/㎖에서 각각 77.32 ± 2.28%, 64.09 ± 1.01%의 활성을 나타냈다. 또한, 리기테다 소나무 솔방울은 산화적 스트레스에 대한 plasmid DNA 보호 효과를 보였다.

Abstract

Reactive oxygen species (ROS) damage DNA and cause cancer. Therefore, the research is being conducted on the development of antioxidants for the removal of ROS. This study was performed to investigate antioxidant activity and protective effect against oxidative DNA damage using ethyl acetate fractions from the cone of Pinus rigida x taeda (ERT). The antioxidant activity was evaluated using the DPPH, ABTS radical scavenging assay, reducing power assay, and Fe2+ chelating assay. Also, the contents of phenolic compounds and vitamin C related to antioxidant activity were analyzed to confirm phytochemicals. The DNA protective effect against oxidative stress was confirmed by the φX-174 RF I plasmid DNA cleavage assay. As a result, ERT showed DPPH and ABTS radical scavenging activities in a concentration-dependent manner. The results of reducing power and Fe2+ chelating activities were 77.32 ± 2.28% and 64.09 ± 1.01% at 200 ㎍/㎖. Also, ERT showed a DNA protective effect against oxidative stress.

참고문헌 (41)

  1. S. D. Aust, "Free radicals in toxicology", Toxicology and applied pharmacology, 120(2), pp.168~178 (1993)[DOI]
  2. S. J. Stohs, "The role of free radicals in toxicity and disease", Journal of basic and clinical physiology and pharmacology, 6(3-4), pp.205~228 (1995)[DOI]
  3. L. J. Marnett, "Oxyradicals and DNA damage", Carcinogenesis, 21(3), pp.361~370 (2000)[DOI]
  4. S. R. Maxwell, "Prospects for the use of antioxidant therapies", Drugs, 49(3), pp.345~361 (1995)[DOI]
  5. A. N. T. Kong, "Signal transduction events elicited by cancer prevention compounds", Mutation Research, 2001, pp.480, 231~241
  6. H. Wiseman, "Damage to DNA by reactive oxygen and nitrogen species : role in inflammatory disease and progression to cancer", Biochemical Journal, 313(1), pp.17~29 (1996)[DOI]
  7. 정성제, "약용 식물 추출물의 항산화 활성 검색", Applied Biological Chemistry, 47(1), pp.135~140 (2004), 한국응용생명화학회
  8. 부희옥, "식물유래 천연색소의 항산화 활성", 한국자원식물학회지, 24(1), pp.105~112 (2011), 한국자원식물학회
  9. 차배천, "청미래덩굴 잎의 항산화 활성 성분", 생약학회지, 38(1), pp.31~36 (2007), 한국생약학회
  10. M. Asensi, "Natural polyphenols in cancer therapy", Critical reviews in clinical laboratory sciences, 48(5-6), pp.197~216 (2011)[DOI]
  11. 이양숙, "민자주방망이버섯의 폴리페놀 함량과 항산화활성", 한국식품영양과학회지, 35(10), pp.1309~1314 (2006), 한국식품영양과학회
  12. B. S. An, "Anti-inflammatory effects of essential oils from Chamaecyparis obtusa via the cyclooxygenase-2 pathway in rats", Molecular medicine reports, 8(1), pp.255~259 (2013)[DOI]
  13. J. H. Kuk, "Isolation and characterization of benzoic acid with antimicrobial activity from needle of Pinus densiflora", Korean Journal of Food Science and Technology, 29(2), pp.204~210 (1997)
  14. A. Otto, "Terpenoids as chemosystematic markers in selected fossil and extant species of pine(Pinus, Pinaceae)", Botanical Journal of the Linnean Society, 154(1), pp.129~140 (2007)[DOI]
  15. 장태원, "솔방울 에틸아세테이트 분획물의 항산화 및 산화적 DNA 손상 억제 활성", 한국자원식물학회지, 29(2), pp.163~170 (2016), 한국자원식물학회[DOI]
  16. 정경희, "미성숙 솔방울 열수추출물의 항균성", 한국염색가공학회지, 26(1), pp.45~52 (2014), 한국염색가공학회
  17. 강수경, "Phytoncide Extracted from Pinecone Decreases LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells", Journal of Microbiology and Biotechnology, 26(3), pp.579~587 (2016), 한국미생물·생명공학회[DOI]
  18. C. S. Ku, "Exploitation of polyphenol-rich pine barks for potent antioxidant activity", Journal of Wood Science, 53(6), pp.524~528 (2007)[DOI]
  19. V. Bondet, "Kinetics and mechanisms of antioxidant activity using the DPPH free radical method", Lebensm. -Wiss. u. -Technol, 30, pp.609~615 (1997)
  20. R. Van den Berg, "Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chemistry, 66(4), pp.511~517 (1999)[DOI]
  21. M. Oyaizu, "Studies on products of browning reaction", The Japanese journal of nutrition and dietetics, 44(6), pp.307~315 (1986)[DOI]
  22. B. Hus, "Antioxidant activity of hot water extract from the fruit of the Doum palm", Hyphaene thebaica. Food Chemistry, 98(2), pp.317~328 (2006)[DOI]
  23. AOAC, "Official Methods of Analysis" (1990), Association of Official Analytical Chemists
  24. S. K. Jagota, "A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent", Analytical biochemistry, 127(1), pp.178~182 (1982)[DOI]
  25. H. E. Seifried, "A review of the interaction among dietary antioxidants and reactive oxygen species", The Journal of nutritional biochemistry, 18(9), pp.567~579 (2007)[DOI]
  26. K. S. Kasprzak, "Possible role of oxidative damage in metal-induced carcinogenesis", Cancer investigation, 13(4), pp.411~430 (1995)[DOI]
  27. M. Lu, "Antioxidant capacity and major phenolic compounds of spices commonly consumed in China", Food Research International, 44(2), pp.530~536 (2011)[DOI]
  28. K. Thaipong, "Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts", Journal of food composition and analysis, 19(6-7), pp.669~675 (2006)[DOI]
  29. 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]
  30. S. Meir, "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves", Journal of agricultural and food chemistry, 43(7), pp.1813~1819 (1995)[DOI]
  31. S. Sakanaka, "Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea(kakinoha-cha)", Food chemistry, 89(4), pp.569~575 (2005)[DOI]
  32. S. J. Stohs, "Oxidative mechanism in the toxicity of metal ions", Free Radic. Biol. Med, 18(2), pp.321~336 (1995)[DOI]
  33. X. Huang, "Ferrous ion autoxidation and its chelation in iron-loaded human liver HepG2 cells", Free Radical Biology and Medicine, 32(1), pp.84~92 (2002)[DOI]
  34. T. Tanaka, "Induction of embryonal carcinoma cell differentiation by deferoxamine, a potent therapeutic iron chelator", Biochimica et Biophysica Acta(BBA)-Molecular Cell Research, 1357(1), pp.91~97 (1997)[DOI]
  35. A. Ardestani, "Antioxidant and free radical scavenging potential of Achillea santolina extracts", Food chemistry, 104(1), pp.21~29 (2007)[DOI]
  36. B. Halliwell, "The characterization of antioxidants", Food and Chemical Toxicology, 33(7), pp.601~617 (1995)[DOI]
  37. C. Rice-Evans, "Antioxidant properties of phenolic compounds", Trends in plant science, 2(4), pp.152~159 (1997)[DOI]
  38. 장태원, "미선나무 미성숙 종자의 항산화 및 미백 활성", 생명과학회지, 27(5), pp.536~544 (2017), 한국생명과학회
  39. J. E. Klaunig, "The role of oxidative stress in chemical carcinogenesis", Environmental health perspectives, 106(suppl 1), pp.289~295 (1998)[DOI]
  40. Y. J. Jung, "Oxidative DNA damage and cytotoxicity induced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid", Free Radical Biology and Medicine, 30(12), pp.1407~1417 (2001)[DOI]
  41. E. Graf, "Antioxidant functions of phytic acid", Free Radical Biology and Medicine, 8(1), pp.61~69 (1990)[DOI]

관련 논문AI추천

솔방울 에틸아세테이트 분획물의 항산화 및 산화적 DNA 손상 억제 활성남수환, 박재호, 장태원한국자원식물학회 · 한국자원식물학회지2016 · 제29권 제2호Effects of Abeliophyllum distichum Nakai Flower Extracts on Antioxidative Activities and Inhibition of DNA Damage박재호, 안정좌한국자원식물학회 · 한국자원식물학회지2013 · 제26권 제3호Antioxidant Activity and Inhibitory Effects on Oxidative DNA Damage of Callus from Abeliophyllum distichum Nakai박재호, 장태원한국자원식물학회 · 한국자원식물학회지2018 · 제31권 제3호리기다(Pinus rigida) 솔방울 추출물의 항산화 활성 및 UVB 유발 Hs68 세포 손상 억제 효과김용신, 박재호, 이철규한국자원식물학회 · 한국자원식물학회지2025 · 제38권 제4호솔방울 메탄올 추출물의 항산화 및 멜라닌 생성 억제 효과노성수, 민유홍, 이아름, 이은숙한국피부과학연구원 · 아시안뷰티화장품학술지2016 · 제14권 제3호The Protective and Inhibitory Effect of Antioxidants Found in Broussonetia kazinoki Siebold against Oxidative DNA Damage권태형, 김도완, 김희기, 박재호, 안정좌, 이기범, 이은자, 장태원, 최지수한국자원식물학회 · 한국자원식물학회지2019 · 제32권 제6호

이 간행물의 다른 논문 (39건)

단일 이미지 패턴을 이용한 다수의 전력설비 데이터를 증강하기 위한 패턴 배열화 기법 설계김석수pp. 1-8MIM 적용을 통한 IoT 기반 무선 센서 네트워크 성능 최대화 방안강영명pp. 9-15증강현실을 이용한 전력 설비 모니터링 시스템의 데이터 교환 기법 설계김석수, 김동현pp. 16-22MIMO 기반의 IoT 통신 잡음을 최소화하기 위해서 딥러닝을 활용한 비밀키 차원 분배 메커니즘조성남, 정윤수pp. 23-29Efficient Operation and Management Scheme of Micro Data Centers for Realization of Edge Computing최정열pp. 30-39
원문 보기