본문 바로가기
KERD
논문KCI 등재

Modulation of Pro-inflammatory and Anti-inflammatory Cytokines in the Fat by an Aloe Gel-based Formula, QDMC, Is Correlated with Altered Gut Microbiota

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Abnormal inflammatory responses are closely associated with intestinal microbial dysbiosis. Oral administration of Qmatrix-diabetes-mellitus complex (QDMC), an Aloe gel-based formula, has been reported to improve inflammation in type 2 diabetic mice; however, the role of the gut microbiota in ameliorating efficacy of QDMC remains unclear. We investigated the effect of QDMC on the gut microbiota in a type 2 diabetic aged mouse model that was administered a high-fat diet. Proinflammatory (TNF-α and IL-6) and anti-inflammatory (IL-4 and IL-10) cytokine levels in the fat were normalized via oral administration of QDMC, and relative abundances of Bacteroides, Butyricimonas, Ruminococcus, and Mucispirillum were simultaneously significantly increased. The abundance of these bacteria was correlated to the expression levels of cytokines. Our findings suggest that the immunomodulatory activity of QDMC is partly mediated by the altered gut microbiota composition.

Abstract

Abnormal inflammatory responses are closely associated with intestinal microbial dysbiosis. Oral administration of Qmatrix-diabetes-mellitus complex (QDMC), an Aloe gel-based formula, has been reported to improve inflammation in type 2 diabetic mice; however, the role of the gut microbiota in ameliorating efficacy of QDMC remains unclear. We investigated the effect of QDMC on the gut microbiota in a type 2 diabetic aged mouse model that was administered a high-fat diet. Proinflammatory (TNF-α and IL-6) and anti-inflammatory (IL-4 and IL-10) cytokine levels in the fat were normalized via oral administration of QDMC, and relative abundances of Bacteroides, Butyricimonas, Ruminococcus, and Mucispirillum were simultaneously significantly increased. The abundance of these bacteria was correlated to the expression levels of cytokines. Our findings suggest that the immunomodulatory activity of QDMC is partly mediated by the altered gut microbiota composition.

참고문헌 (38)

  1. Cani PD, "Metabolic endotoxemia initiates obesity and insulin resistance", Diabetes, 56, pp.1761~1772 (2007)
  2. Lee H, "Amelioration of hyperglycemia by Rubus occidentalis(black raspberry)and increase in short-chain fatty acids producing bacteria", J Funct Foods, 54, pp.433~439 (2019)
  3. Yagi A, "Immune modulation of Aloe vera : acemannan and gut microbiota modulator", J Gastroenterol Hepatol Res, 4, pp.1707~1721 (2015)
  4. Song JX, "Dietary capsaicin improves glucose homeostasis and alters the gut microbiota in obese diabetic ob", ob Mice, 2017, pp.8, 602~
  5. Everard A, "Cross-talk between Akkermansia muciniphila and intestinal epithelium controls dietinduced obesity", Proc Natl Acad Sci U S A, 110, pp.9066~9071 (2013)
  6. Lee H, "Effect of metformin on metabolic improvement and gut microbiota", Appl Environ Microbiol, 80, pp.5935~5943 (2014)
  7. Chen Y, "Gut metagenomes of type 2 diabetic patients have characteristic single-nucleotide polymorphism distribution in Bacteroides coprocola", Microbiome, 5, pp.15~ (2017)
  8. Pedersen C, "Fecal Enterobacteriales enrichment is associated with increased in vivo intestinal permeability in humans", Physiol Rep, 6, pp.e13649~ (2018)
  9. Ramachandra CT, "Processing of Aloe vera leaf gel : a review", Am J Agric Biol Sci, 3, pp.502~510 (2008)
  10. Lee Y, "Aloe QDM complex enhances specific cytotoxic T lymphocyte killing in vivo in metabolic disease mice", Biosci Biotechnol Biochem, 81, pp.595~603 (2017)
  11. Kim K, "Hypoglycemic and hypolipidemic effects of processed Aloe vera gel in a mouse model of non-insulin-dependent diabetes mellitus", Phytomedicine, 16, pp.856~863 (2009)
  12. 신은주, "Dietary Aloe Reduces Adipogenesis via the Activation of AMPK and Suppresses Obesity-related Inflammation in Obese Mice", Immune Network, 11(2), pp.107~113 (2011), 대한면역학회
  13. 김경제, "Down-Regulation of Adipogenesis and Hyperglycemia in Diet-Induced Obesity Mouse Model by Aloe QDM", Biomolecules & Therapeutics, 18(3), pp.336~342 (2010), 한국응용약물학회
  14. Gilbert JA, "Meeting report: the terabase metagenomics workshop and the vision of an earth microbiome project", Stand Genomic Sci, 3, pp.243~248 (2010)
  15. Caporaso JG, "QIIME allows analysis of high-throughput community sequencing data", Nat Methods, 7, pp.335~336 (2010)
  16. Segata N, "Metagenomic biomarker discovery and explanation", Genome Biol, 12, pp.R60~ (2011)
  17. Yee CS, "Enhanced production of IL-10 by dendritic cells deficient in CIITA", J Immunol, 174, pp.1222~1229 (2005)
  18. Müller A, "Differential regulation of Th1-type and Th2-type cytokine profiles in pancreatic islets of C57BL", 6 and BALB, Immunobiology, pp.2002, 205[DOI]
  19. Hamman JH, "Composition and applications of Aloe vera leaf gel", Molecules, 13, pp.1599~1616 (2008)
  20. Gullón B, "In vitro assessment of the prebiotic potential of Aloe vera mucilage and its impact on the human microbiota", Food Funct, 6, pp.525~531 (2015)
  21. Nagpal R, "Effect of Aloe vera juice on growth and activities of Lactobacilli invitro", Acta Biomed, 83, pp.183~188 (2012)
  22. Clarke SF, "The gut microbiota and its relationship to diet and obesity : new insights", Gut Microbes, 3, pp.186~202 (2012)
  23. Flint HJ, "Polysaccharide breakdown by anaerobic microorganisms inhabiting the Mammalian gut", Adv Appl Microbiol, 56, pp.89~120 (2004)
  24. Zhang W, "Effects of berberine and metformin on intestinal inflammation and gut microbiome composition in db", db mice, 2019, pp.118, 109131~
  25. Chiu CM, "Systematic analysis of the association between gut flora and obesity through high-throughput sequencing and bioinformatics approaches", BioMed Res Int, 2014, pp.906168~ (2014)
  26. Kim J, "Alterations in gut microbiota by statin therapy and possible intermediate effects on hyperglycemia and hyperlipidemia", Front Microbiol, 10, pp.1947~ (2019)
  27. Herp S, "Mucispirillum schaedleri antagonizes salmonella virulence to protect mice against colitis", Cell Host Microbe, 25, pp.681.e8~694.e8 (2019)
  28. Loy A, "Lifestyle and horizontal gene transfer-mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota", mSystems, 2, pp.e00171~e00116 (2017)
  29. Cawthorn WP, "TNF-α and adipocyte biology", FEBS Lett, 582, pp.117~131 (2008)
  30. Uysal KT, "Protection from obesity-induced insulin resistance in mice lacking TNF-α function", Nature, 389, pp.610~614 (1997)
  31. Ménard S, "Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport", Gut, 53, pp.821~828 (2004)
  32. Hiroko Nagao-Kitamoto, "Host-microbial Cross-talk in Inflammatory Bowel Disease", Immune Network, 17(1), pp.1~12 (2017), 대한면역학회[DOI]
  33. Klover PJ, "Chronic exposure to interleukin-6 causes hepatic insulin resistance in mice", Diabetes, 52, pp.2784~2789 (2003)
  34. Stouthard JM, "Interleukin-6 enhances glucose transport in 3T3-L1adipocytes", Biochem Biophys Res Commun, 220, pp.241~245 (1996)
  35. Kelly M, "AMPK activity is diminished in tissues of IL-6 knockout mice : the effect of exercise", Biochem Biophys Res Commun, 320, pp.449~454 (2004)
  36. Lin SY, "Interleukin-4 improves metabolic abnormalities in leptin-deficient and high-fat diet mice", Int J Mol Sci, 21, pp.4451~ (2020)
  37. Rajbhandari P, "IL-10 signaling remodels adipose chromatin architecture to limit thermogenesis and energy expenditure", Cell, 172, pp.218.e17~233.e17 (2018)
  38. Faulkner JL, "Interleukin-10 deficiency limits the development of obesity and insulin resistance produced by a high fat diet", FASEB J, 27, pp.1183~1186 (2013)

관련 논문AI추천

Dietary Aloe Improves Insulin Sensitivity via the Suppression of Obesity-induced Inflammation in Obese Mice공현석, 권정학, 김경제, 박영인, 신슬미, 신은주, 심규석, 이성원, 이종길, 주태형대한면역학회 · Immune Network2011 · 제11권 제1호Dietary Aloe Reduces Adipogenesis via the Activation of AMPK and Suppresses Obesity-related Inflammation in Obese Mice공현석, 김경제, 도선길, 박영인, 신슬미, 신은주, 이성원, 이종길, 조태형, 황인경대한면역학회 · Immune Network2011 · 제11권 제2호Dietary Aloe QDM Complex Reduces Obesity-Induced Insulin Resistance and Adipogenesis in Obese Mice Fed a High-Fat Diet공현석, 김경제, 김설아, 도선길, 박영인, 신슬미, 신은주, 오희은, 이종길, 조태형대한면역학회 · Immune Network2012 · 제12권 제3호Heat-Killed Lactiplantibacillus plantarum LRCC5314 Mitigates the Effects of Stress- Related Type 2 Diabetes in Mice via Gut Microbiome Modulation김원용, 황광우, Baek Jihye, Kim Jong-Hwa, Nam YoHan, Park Miri, Yoon Seokmin한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제3호IL-10 Expression-Inducing Gut Bacteria Alleviate High-Fat Diet-Induced Obesity and Hyperlipidemia in Mice김동현, 김혜인, 윤수원, 장세은, 한명주한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제4호Downregulation of IL-18 Expression in the Gut by Metformin-induced Gut Microbiota Modulation공현석, 김경제, 김지연, 박일호, 송영천, 안진호, 이성원, 이종길, 이희태, 최도현대한면역학회 · Immune Network2019 · 제19권 제4호

이 간행물의 다른 논문 (5건)