적색, 녹색, 청색광에서 생장한 Paenibacillus hunanensis 의 지구화학적 특성 연구
Study on Geochemical Characteristics of Paenibacillus hunanensis Grown Under Red, Green, and Blue Lights
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초록
이 연구에서는 서로 다른 가시광선 파장 조건하에서 세대를 거듭하여 진화한 박테리아의 생장 특성과 다양한 pH 및 구리 독성에 대한 내성을 평가하였다. 전남대학교 내 토양으로부터 분리한 미생물을 실험 대상으로 하였으며 16s rRNA 결과 Paenibacillus hunanensis와 99.2%의 유사도를 보였다. 분리된 토양 토착균을 이용하여 적색, 녹색, 청색광의 파장에서 31세대에 걸쳐 미생물의 계대배양을 수행하였으며 세대별로 생장한 박테리아의 생물량을 측정하였다. 또한 31세대 후의 박테리아를 이용하여 pH 4, 7, 9 및 구리 함량 10, 100, 1000 ppb 조건에서 생장한 생물량을 확인하였다. 실험 결과, 시간이 경과하며 적색광 조건에서 생장한 박테리아가 높은 활성도를 보였으며, 적색광 조건에서 생장한 박테리아는 pH 4와 7에서 높은 생물량을 나타냈다. 구리에 대한 내성 차이를 관찰한결과, 청색광 조건 및 빛이 없는 조건에서 생장한 박테리아가 낮은 생장량을 보였다.
Abstract
Bacteria that had evolved over multiple generations under different visible light wavelengths were evaluated for their growth characteristics, resistance to various pH levels, and copper toxicity. The microorganism was isolated from soil at Chonnam National University and identified as Paenibacillus hunanensis with a similarity of 99.2%. Microbial culturing over 31 generations was performed under red, green, and blue light wavelengths, and the biomass of the bacteria grown in each generation was measured. Additionally, the biomass of bacteria grown at pH 4, 7, and 9 and copper concentrations of 10, 100, and 1000 ppb were determined using bacteria from the 31st generation. The results showed that the bacteria grown under red light exhibited higher activity over time. Bacteria grown under red light showed higher biomass at pH 4 and 7. Owing to the differences in resistance to copper, bacteria grown under blue light and in the absence of light exhibited lower biomass.
참고문헌 (15)
- Al-Ansari, M. M., "Influence of blue light on effective removal of arsenic by photosynthetic bacterium Rhodobacter sp. BT18", Chemosphere, 292, pp.133399~ (2022)
- Atsumi, S., "Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli", Molecular Systems Biology, 6, pp.449~ (2010)
- Babitha, S., "Effect of light on growth, pigment production and culture morphology of Monascus purpureus in solid-state fermentation", World Journal of Microbiology and Biotechnology, 24, pp.2671~2675 (2008)
- Barrick, J. E., "Genome evolution and adaptation in a long-term experiment with Escherichia coli", Nature, 461, pp.1243~1247 (2009)
- Do, J. S., "Bactericidal effect of 461 nm blue light emitting diode on pathogenic bacteria", Korean Journal of Food Preservation, 20(3), pp.419~423 (2013)
- Do, J. S., "Bactericidal effect of pathogenic bacteria on acid treatment combined with red, green, and blue LED light at a low temperature environment", Journal of the Korean Society of Food Science and Nutrition, 44(11), pp.1725~1732 (2015)
- Govarthanan, M., "Effect of blue light on growth and exopolysaccharides production in phototrophic Rhodobacter sp. BT18 isolated from brackish water", International Journal of Biological Macromolecules, 131(15), pp.74~80 (2019)
- Grady, E. N., "Current knowledge and perspectives of Paenibacillus:a review", Microbial Cell Factories, 15, pp.203~ (2016)
- Kim, S. W., "In vitro bactericidal effects of 625, 525, and 425 nm wavelength (red, green, and blue) light-emitting diode irradiation", Photomedicine and Laser Surgery, 31(11), pp.554~562 (2013)
- "Korean Statistical Information Service (KOSIS)" (2024)[URL]
- Lee, D. H., "Evolution of microbial laboratory evolution", The Microorganisms and Industry, 39(2), pp.6~8 (2013)
- Liu, Y., "Paenibacillus hunanensis sp. nov., isolated from rice seeds", International Journal of Systematic and Evolutionary Microbiology, 60(6), pp.1266~1270 (2010)
- Moon, J. S., "Inactivation of bacterial pathogens by irradiation of red, green, blue and combined light-emitting diode (LED)", Korean Society for Biotechnology and Bioengineering Journal, 28(6), pp.428~432 (2013)
- Ultraviolet Disinfection Guidance Manual for the Final Long Term 2 Enhanced Surface Water Treatment Rule, "United States Environmental Protection Agency (US EPA)", 44~52, pp.EPA 815-R-06-007 (2006)
- Young, A. R., "Acute effects of UVR on human eyes and skin", Progress in Biophysics and Molecular Biology, 92(1), pp.80~85 (2006)