국내 원유 세균수 검사장비 박토스캔 및 박토카운트의 보정식 재평가
Re-evaluation on conversion system of BactoScan and BactoCount for raw milk in South Korea
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초록
The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacte-rial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evalu-ated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000∼1,000,000 CFU/mL from June 2020 to Octo-ber 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coef-ficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to con-tribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.
Abstract
The total bacteria count is significantly important factor for hygienic quality in raw milk. BactoScan FMTM and BactoCount IBCTM are the automated instruments for the determination of the total bacte-rial count in raw milk. They have been used after calibration by standard plate count (SPC) method in South Korea since 2000. It is necessary to re-evaluate for total bacterial counter according to the improvement of milk quality and the change of milk quality grade. Therefore, this study was evalu-ated the conversion mode of the two machines by SPC method. We collected 921 bulk-tank milk samples throughout the concentration range of 1,000∼1,000,000 CFU/mL from June 2020 to Octo-ber 2021. As a result, when compared by the SPC value, there was a slight difference in total bacterial count in BactoScan below 10,000 CFU/mL and above 200,000 CFU/mL and in BactoCount above 100,000 CFU/mL, respectively. Therefore, the conversion factor for BactoScan and the conversion equation for BactoCount were newly adjusted based on SPC value, and then the correlation coef-ficients (R2) was 0.85 or higher. In addition, the correlation (R2) between BactoScan and BactoCount was 0.91, which means the results were high positive correlation. These results are expected to con-tribute to improving the accuracy of the automated instruments for determining of total bacterial count in raw milk.
참고문헌 (21)
- 농림축산검역본부, "원유검사 표준화 운영 실무 가이드라인", 43~52 (2019)
- 농림축산검역본부, "2020년 원유검사 표준화 교육", 41~48 (2020)
- 농림축산검역본부, "원유검사 표준용액 제조 및 사용지침서", 101~125 (2021)
- Bolzoni G, "BactoScan FC: conversion system for results at the national level in Italy and reproducibility of total bacterial counting four years after implementation", Euroreference, 46~54 (2017)
- Cunningham JD, "Collaborative study of the bactoscan-an automated method for determinations of total bacteria in raw milk", Canadian Institute of Food Science and Technology Journal, 21(5), pp.464~466 (1988)
- Desmeasures NM, "Monitoring the microbiology of high quality milk by monthly sampling over 2 years", J Dairy Res, 64, pp.271~280 (1997)
- Gunasekera TS, "A flow cytometry method for rapid detection and enumeration of total bacterial bacteria in milk", Appl Environ Microbiol, 66, pp.1228~1232 (2000)
- Gunasekera TS, "Potential for broad applications of flow cytometry and fluorescence technique in microbiological and somatic cell analysis of milk", Int J Food Microbiol, 85(3), pp.269~279 (2003)
- IDF, "Milk and milk products enumeration of microorganisms colony count technique at 37℃. FILIDF standard no 100B", ~ (1991)
- IDF, "Milk payment systems for ex-farm milk. no 3331", ~ (1998)
- IDF, "Milk – Quantitive determination of microbiological quality – Guidance on establishing and verifying a conversion relationship between routine method results and anchor method results. FIL-IDF standard no 196", ~ (2004)
- Hye Jeong Kang, "원유 세균수 검사장비의 정도관리를 위한 표준시료의 개발 및 평가", 한국동물위생학회지, 43(3), pp.147~154 (2020), 한국동물위생학회[DOI]
- Madden RH, "Determination of the BactoScan conversion Factor for the United Kingdom", Dairy microbiology, 70, pp.17~19 (2017)
- "REGULATION(EC) No 853", 2004 of the European parliament and of the council of 29 April 2004 laying down specific hygiene rules for the hygiene of foodstuffs
- Suhren G, "First experiences with automatic flow cytometric determination of total bacterial count in raw milk", Bulletin of the International Dairy Federation, 50, pp.249~275 (2000)
- Walter S, "Application of a unified European conversion factor for the transformation of Bactoscan FC values", Food Agroscope Science, 36, pp.1~11 (2016)