배추과 유전자원 종자의 항산화활성 및 바이오활성 화합물 함량 변이
Variation of Antioxidant Activity and Bioactive Compounds Content in Cruciferae Seeds
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본 연구의 목적은 배추과 종자들의 고 함유 바이오 활성 화합물 배추속 및 무속의 유전자원들을 선발하고, 이를 활용한 고부가가치 기능성 상품 개발을 위한 기초 자료를 제공하는데 있다. 배추속(양배추,녹색꽃양배추, 콜라비, 꽃양배추) 및 무속(무) 유전자원 총 92점을 대상으로 라디컬 소거능을 이용한 항산화활성과 폴리페놀 및 플라보노이드 함량을 측정하였다. 배추속 및 무속 종자들의 항산화활성과 플라보노이드 함량의 평균간에는 유의차가 있었다. 배추속 변종들간에도 항산화활성, 폴리페놀 및 플라보노이드 함량이 통계적으로 유의차가 있었다.‘Tropic Sun Plus(B8)’양배추 등 총 22점의 배추속 품종및 계통을 항산화활성이 우수한 품종 및 계통으로 선발하였고 시판 무종자인‘Taeyang(R43)’과 육성계통인‘13-FM 136-5×13-FM 138-1(R33)’의 항산화활성이 각각 142% 및 157%으로 실험한 유전자원들의 종자 중 가장 높았다. 폴리페놀의 함량이 높은 배추속 유전자원은‘Tropic Sun Plus(B8)’양배추 등 총 10점이였고, 국립원예특작과학원 채소과에서 육성한 무 계통인‘13-FH 20-1(R4)’및‘13-FH 20-2(R5)’의 폴리페놀 함량이 높았다.‘Megaton(B14)’양배추 등 총 14점의 배추속 품종 및계통을 플라보노이드 함량이 높은 품종 및 계통으로 선발하였다.‘13-FH 20-2(R5)’무의 플라보노이드 함량은 1,000mg·100g-1이상으로 플라보노이드 고 함유 종자로 활용가치가 높은 계통이였다. 이상의 결과로 항산화활성이 높고 바이오 활성 화합물의 함량이 높은 유전자원들을 선발하였고, 유전자원들을 종자 기능성 가공품으로 활용하여 부가가치를 높히는데 기여할 것이라 판단되었다.
Abstract
This study was aimed to select seeds of Brassica and Raphanus germplasm in terms ofsuperior with bioactive compounds content and to inform sophisticated data for developing thehigh value-added products. We estimate to aspects of the antioxidant activiy, polyphenol, andflavonoid contents of seeds from the total 92 cultivars and breeding lines in Brassica(cabbage,brocoli, kohlrabi, and cauliflower) and Raphanus(raddish) germplasm. There are singificantdifferences between physiological functonalites of Brassica and Raphanus. The antioxidantactivity, contents of polyphenol and flavonoid were differed according to variation of Brasscia. ‘Tropic Sun Plus(B8)’ cabbage seeds and other 22 cultivars and breeding lines in Brassica seeds were selected to aspects of superior antioxidant activity. The antioxidant activities of ‘13-FM136-5×13-FM 138-1 (R33)’ and ‘Taeyang (R43)’ radish seeds were 142% and 157%,respectively, which were greater than other cutlivars and breeding lines of radish seeds. Thepolyphenol contents of ‘Tropic Sun Plus(B8)’ cabbage seed and other 10 cultivars and breedinglines’s seeds were the greatest among Brasscia germplasm, while those of ‘13-FH 20-1 (R4)’and ‘13-FH 20-2 (R5)’ radish seeds were superior than other radish seeds. ‘Megaton(B14)’cabbage seed and other 14 cultivars and breeding lines in Brassica seeds were represented asthe contain high amounts of flavonoid. In addition, the flavonoid content of ‘13-FH 20-2 (R5)’radish seed showed over 1,000mg·100g-1, which was the greatest among all the tested radishgermplasm. Results revealed that there was significant variation of antioxidant activity andbioactive compounds contents in Cruciferae seeds and the selected cultivars and breeding lines’sseeds can be used to materials for developing high value-added products.
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