Mate (Ilex paraguarensis) 에탄올 추출물의 추출조건 최적화
Optimization of Extraction Conditions for Mate (Ilex paraguarensis) Ethanolic Extracts
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초록
This study was conducted to monitor the quality characteristics of mate (Ilex paraguarensis) ethanolic extracts via the response surface methodology. In the extraction conditions that were based on the central composite design with variations in the ethanol concentration (0-100%), extraction temperature(35-95℃), and the ratio of the solvent to the sample (10～30 mL/g). The extraction yield and total polyphenol content improved with the increase in the ethanol concentration than in the extraction temperature. The caffeic acid content increased with the decrease in the solvent ratio. The coefficients of determinations (R2) were 0.8842 (p<0.05), 0.8729 (p<0.05), and 0.9205 (p<0.05) in terms of the electron donating ability, nitrite scavenging effect (pH 3.0), and SOD-like ability, respectively. The estimated conditions for the maximized extraction, including in terms of the yield, total polyphenol content, caffeic acid content, and electron donating ability, were a 21-48% ethanol concentration, a 76.4℃ extraction temperature, and 10-14mL/g solvent-to-sample ratio.
Abstract
This study was conducted to monitor the quality characteristics of mate (Ilex paraguarensis) ethanolic extracts via the response surface methodology. In the extraction conditions that were based on the central composite design with variations in the ethanol concentration (0-100%), extraction temperature(35-95℃), and the ratio of the solvent to the sample (10～30 mL/g). The extraction yield and total polyphenol content improved with the increase in the ethanol concentration than in the extraction temperature. The caffeic acid content increased with the decrease in the solvent ratio. The coefficients of determinations (R2) were 0.8842 (p<0.05), 0.8729 (p<0.05), and 0.9205 (p<0.05) in terms of the electron donating ability, nitrite scavenging effect (pH 3.0), and SOD-like ability, respectively. The estimated conditions for the maximized extraction, including in terms of the yield, total polyphenol content, caffeic acid content, and electron donating ability, were a 21-48% ethanol concentration, a 76.4℃ extraction temperature, and 10-14mL/g solvent-to-sample ratio.
참고문헌 (35)
- 고윤정, "항산화 활성 증진을 위한 민들레 잎의 열수추출 조건의 최적화", 한국식품과학회지, 40(3), pp.283~289 (2008), 한국식품과학회
- Halliwell B, "DNA damage by oxygen-derived species", FEBS Lett, 281, pp.9~19 (1991)
- Jennings PE, "New approaches to the pathogenesis and treatment of diabetic microangiopathy", Diabetic Med, 5, pp.111~117 (1988)
- Fridovich I, "Superoxide dismutase an adaption to paramagnetic gas", J Biol Chem, 264, pp.7761~7762 (1989)
- Jacques RA, "Extraction of purine alkaloids from Maté (Ilex Paraguariensis) using supercritical CO2", J Agric Food Chem, 55, pp.7510~7516 (2007)
- Katia HK, "Mate saponin 5, a highly polar saponin from ILEX Paraguariensis", Phytochemistry, 42, pp.1119~1122 (1996)
- Rosangela AJ, "Pressurized liquid extraction of mate tea leaves", Anal Chim Acta, 625, pp.70~76 (2008)
- Manuella L, "Mate tea reduced acute lung inflammation in mice exposed to cigarette smoke", Nutrition, 24, pp.375~381 (2008)
- Heck CI, "Yerba mate tea (Ilex Paraguariensis): A comprehensive review on chemistry, health implications, and technological consideration", J Food Sci, 72, pp.138~151 (2007)
- Jack LA, "Naturally occurring proteasome inhibitors from mate tea (Ilex Paraguariensis) serve as models for topical proteasome inhibitors", J Invest Dermatol, 125, pp.207~212 (2005)
- 홍주헌, "Mate (ILex paraguarensis) 발효차의 품질특성", 한국식품저장유통학회지, 17(4), pp.500~506 (2010), 한국식품저장유통학회
- Rosana F, "Antioxidant activity of Ilex paraguariensis and related species", Nutrition Res, 20, pp.1437~1446 (2000)
- Euclides LC, "Methylxanthines and phenolic compounds in mate (Ilex paraguariensis St Hil) progenies grown in Brazil", J Food Composition & Analysis, 20, pp.553~558 (2007)
- Claudia A, "Peroxidase-like activity of Ilex Paraguariensis", Food Chem, 97, pp.459~464 (2006)
- 김현구, "홍월적 양배추의 추출조건 최적화", 한국식품영양과학회지, 34(10), pp.1625~1632 (2005), 한국식품영양과학회
- 김정옥, "반응표면분석법에 의한 무화과 열수 추출조건의 최적화", 한국식품저장유통학회지, 15(1), pp.66~73 (2008), 한국식품저장유통학회
- Gontard N, "Edible wheat gluten films: Influence of the main process variables on film properties using response surface methodology", J Food Sci, 57, pp.190~196 (1992)
- Lee GD, "Application of response surface methodology in food industry", Food and Industry, 33, pp.33~45 (2000)
- SAS, "SAS User's Guide" (1990), Statistical Analysis Systems Institute
- AOAC, "Official methods of analysis, Fifteenth edition", pp.1010~1011 (1990)
- Singleton VL, "Colorimetry of total phenolics with phenolics with phosphomolybdic- phosphotungstic acid reagents", Am J Enol Viticult, 16, pp.144~158 (1965)
- 이은진, "포도씨 추출물의 총 카테킨 함량과 전자공여능에 대한 마이크로웨이브 추출조건 최적화", 한국식품저장유통학회지, 15(6), pp.840~846 (2008), 한국식품저장유통학회
- Gray JI, "Inhibition of N-nitrosamine formation in model food system", J Food Sci, 40, pp.981~984 (1975)
- Kim SM, "The antioxidant ability and nitrite scavenging ability of plant extracts", Korean J Food Sci Technol, 33, pp.626~632 (2001)
- Park NY, "Optimization of extraction conditions for physicochemical properties of ethanol extracts from Chrysanthemum boreale", J Korean Soc Food Sc. Nutr, 27, pp.585~590 (1998)
- 박기재, "반응표면분석법에 의한 메밀(Fagopyrum esculentum M.) 새싹 기능성분의 추출 조건 최적화", 한국식품저장유통학회지, 16(5), pp.734~741 (2009), 한국식품저장유통학회
- Chung MJ, "Dietary phenolic antioxidants, caffeic acid and trolox, protect rainbow trout gill cells from nitric oxide-induced apoptosis", Aquatic Toxicology, 80, pp.321~328 (2006)
- 이현순, "품종별 들깻잎 열수추출물의 caffeic acid와 rosmarinic acid의 함량 및 항산화력 비교", 한국식품과학회지, 41(3), pp.302~306 (2009), 한국식품과학회
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 26, pp.1199~1204 (1958)
- 윤성란, "복분자 추출조건에 따른 페놀성 화합물의 특성변화", 한국식품영양과학회지, 32(3), pp.338~345 (2003), 한국식품영양과학회
- 노광석, "미나리과 산채의 아질산염 소거능", 한국식품조리과학회지, 18(1), pp.8~12 (2002), 한국식품조리과학회
- Davies R, "The catalysis of the N-nitrosamine of secondary amines by nitrosophenols", J Food Chem, 6, pp.115~122 (1980)
- Kang YH, "The nitrite scavenging and electron donating ability of phenolic compounds", Korean J Food Sci Technol, 28, pp.232~239 (1996)
- Lee SJ, "Effect of natural plant components on the nitrite-scavenging", J Food Hyg Safety, 15, pp.88~94 (2000)
- Kim SM, "Antioxidant of pine extracts according to preparation method", Korean J Food Sci Technol, 31, pp.527~534 (1999)