돼지유래 Salmonella Schwarzengrund의 약제내성 유전자에 관한 연구
Investigation on antimicrobial resistance genes of Salmonella Schwarzengrund isolated from pigs
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (b-lactamase blaTEM), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.
Abstract
To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (b-lactamase blaTEM), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.
참고문헌 (17)
- 김상윤, "경북지역 가축에서 분리된 Salmonella속균의 역학적 특성 및 병원성", 박사, 미생물학, pp.2001
- 이우원, "소와 돼지유래 Salmonella속 균의 혈청형 및 약제감수성", 한국가축위생학회지, 32(1), pp.49~59 (2009), 한국가축위생학회
- 이우원, "소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성", 한국가축위생학회지, 32(1), pp.61~76 (2009), 한국가축위생학회
- 정대영, "전남지역 도축장에서 분리된 Salmonella Typhimurium의 병원성에 관한 연구", 한국 가축위생학회지, 26, pp.39~50 (2003)
- Bolton LF., "Detection of multidrug-resistant Salmonella entericaserotype typhimurium DT104 based on a gene whichconfers cross-resistance to florfenicol and chloramphenicol", J Clin Microbiol, 37, pp.1348~1351 (1999)
- Carlson SA., "Detection of multiresistant Salmonella Typhimurium DT104 by multiplex and fluoregenic PCR", Molecular Cellular Probes, 13, pp.213~222 (1999)
- Chen S., "Characterization of multiple- antimicrobial-resistant Salmonella serovars isolated from reptail meats", Appl Environ Microbiol, 70, pp.1~7 (2004)
- Chiu CH., "Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay", J Clin Microbiol, 34, pp.2619~2622 (1996)
- Edwards PR., "Salmonellosis", Adv Vet Sci, 11, pp.1~63 (1967)
- Gahring LC., "Invasion and replication of Salmonella Typhimurium in animal cells", Infect Immun, 58, pp.443~448 (1990)
- Gebreyes WA., "Molecular characterization of multidrug- resistant Salmonella enterica subsp. enterica serovar Typhimurium isolates from swine", J Clin Microbiol, 40, pp.2813~2822 (2002)
- Khan AA., "Detection of multidrug-resistant Salmonella Typhimurium DT104 by multiplex polymerase chain reaction", FEMS Microbiol Lett, 182, pp.355~360 (2000)
- Llanes C., "Propagation of TEMand PSE-type β-lactamase among amoxicillin-resistant Salmonella spp. isolated in France", Antimicrob Agents Chemother, 43, pp.2430~2436 (1999)
- Pezzella C., "Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolates from animals in Italy", Antimicrob Agents Chemother, 48, pp.903~908 (2004)
- Randall LP., "Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK", J Antimicrob Chemother, 53, pp.208~216 (2004)
- Swamy SC., "Virulence determinants invA and spvC in Salmonella isolated from poultry products, wastewater, and human sources", Appl Environ Microbiol, 62, pp.3768~3771 (1996)
- Yang SJ., "Multidrug-resistant Salmonella Typhimurium and Salmonella Enteritidis identified by multiplex PCR from animals", J Vet Sci, 2, pp.181~188 (2001)