Effect of Codonopsis pilosula polysaccharide on the quality of sheep semen preservation at 4°C
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Objective: This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4°C.Methods: Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1,000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4°C for 24, 72, 120, and 168 h. Sperm acrosome integrity rate was analyzed by Giemsa staining at 24, 72, and 120 h, and mitochondrial membrane integrity was analyzed by Mito-Tracker Red CMXRos. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of spermatozoa were measured after 120 h of preservation.Results: The sperm viability and forward-moving sperm under 200 mg/L CPP were significantly higher than that in the control group at 72 h (61.28%±3.89% vs 52.83%± 0.70%, 51.53%±4.06% vs 42.84%±1.14%), and 168 h (47.21%±0.85% vs 41.43%±0.37%, 38.68%±0.87% vs 31.68%±0.89%). The percentage of fast-moving sperm (15.03%±1.10% vs 11.39%±1.03%) and slow-moving sperm (23.63%±0.76% vs 20.29%±1.11%) in the 200 mg/L group was significantly higher than control group at 168 h. The mitochondrial membrane integrity of the sperm in the group with 200 mg/L CPP was significantly higher than those in the control group after storage at 4°C for 120 h (74.76%±2.54% vs 65.67% ±4.51%, p<0.05). The acrosome integrity rate in the group with 200 mg/L (87.66%±1.26%) and 400 mg/L (84.00%±2.95%) was significantly higher than those in the control group (80.65%±0.16%) after storage for 24 h (p<0.05). CPP also increased T-AOC and decreased the MDA concentration after preservation at 4°C (p<0.05).Conclusion: Adding CPP could improve the T-AOC of sperm, inhibit lipid peroxidation, and facilitate semen preservation.
Abstract
Objective: This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4°C. Methods: Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1,000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4°C for 24, 72, 120, and 168 h. Sperm acrosome integrity rate was analyzed by Giemsa staining at 24, 72, and 120 h, and mitochondrial membrane integrity was analyzed by Mito-Tracker Red CMXRos. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of spermatozoa were measured after 120 h of preservation. Results: The sperm viability and forward-moving sperm under 200 mg/L CPP were significantly higher than that in the control group at 72 h (61.28%±3.89% vs 52.83%± 0.70%, 51.53%±4.06% vs 42.84%±1.14%), and 168 h (47.21%±0.85% vs 41.43%±0.37%, 38.68%±0.87% vs 31.68%±0.89%). The percentage of fast-moving sperm (15.03%±1.10% vs 11.39%±1.03%) and slow-moving sperm (23.63%±0.76% vs 20.29%±1.11%) in the 200 mg/L group was significantly higher than control group at 168 h. The mitochondrial membrane integrity of the sperm in the group with 200 mg/L CPP was significantly higher than those in the control group after storage at 4°C for 120 h (74.76%±2.54% vs 65.67% ±4.51%, p<0.05). The acrosome integrity rate in the group with 200 mg/L (87.66%±1.26%) and 400 mg/L (84.00%±2.95%) was significantly higher than those in the control group (80.65%±0.16%) after storage for 24 h (p<0.05). CPP also increased T-AOC and decreased the MDA concentration after preservation at 4°C (p<0.05). Conclusion: Adding CPP could improve the T-AOC of sperm, inhibit lipid peroxidation, and facilitate semen preservation.
참고문헌 (22)
- Wang YH, "Effect of bovine serum albumin on the effect of preservation of lake sheep semen", Chin J Anim Husb, 57, pp.165~169 (2021)[DOI]
- Eslami M, "Influence of idebenone on ram semen quality stored at 4 °C", Reprod Domest Anim, 54, pp.486~497 (2019)[DOI]
- Abdel-Khaled AE, "Effect of aqueous extracts of moringa oleifera leaves or arctium lappa roots on lipid peroxidation and membrane integrity of ram sperm preserved at cool temperature", J Anim Poult Prod, 7, pp.467~473 (2016)[DOI]
- Li FZ, "Effect of grape seed proanthocyanidins on the effect of preservation of goat semen", J Domest Anim Ecol, 41, pp.59~63 (2020)[DOI]
- Guo LY, "Effect of adding vitamin C to dilution solution on the effect of preservation of Hu sheep semen", Livest Vet Med, 53, pp.18~23 (2021)
- Li SW, "Antioxidant and hypoglycemic activities of Radix et Rhizoma Ginseng", Clinical Medicine Research and Practice, 5, pp.8~11 (2020)
- Zhang T, "Isolation and purification of polysaccharides from Radix Codonopsis pilosulae and their anti-aging effects", Jiangsu Agric Sci, 46, pp.235~237 (2018)
- Meng NN, "Effect of skim milk powder and soy lecithin on the effect of sheep semen preservation", pp.2015
- Lu TT, "Effect of three herbal polysaccharides on the effect of 4°C preservation of sheep semen", pp.2021
- Pan Y, "Research progress on the effect of antioxidants on the preservation of goat semen", J Domest Anim Ecol, 4, pp.6~11 (2020)[DOI]
- Shannon P, "Location of aromatic L-amino acid oxidase in bovine dead semen and differences in the percentage of dead semen determined by oxidase activity between bulls", Guizhou Anim Husb Vet Sci, 02, pp.75~78 (1983)
- Cong GL, "Progress of research and application of antioxidants in sheep semen preservation", Chin J Anim Husb, 56, pp.1~7 (2020)
- Zhao JQ, "Ram semen preserved at 0°C with soybean lecithin Tris-based extender substituted for egg yolk", Asian-Australas J Anim Sci, 34, pp.192~197 (2021)[DOI]
- Liu MQ, "Structural characterization, antioxidant activity, and the effects of Codonopsis pilosula polysaccharides on the solubility and stability of flavonoids", J Pharm Biomed Anal, 229, pp.115368~ (2023)[DOI]
- Xu SF, "Study on the antioxidant effect of Tibetan ginseng polysaccharide on testicular mesenchymal cells", Lishizhen Medicine and Materia Medica Research, 23, pp.1593~1595 (2012)[DOI]
- Zhang JL, "Effect of compound Radix Codonopsis pilosulae polysaccharide extract on the SOD activity of fibroblasts", J Beihua Univ(Natur Sci), 19, pp.205~208 (2018)
- Jin JQ, "Biological functions of Radix et Rhizoma ginseng polysaccharide and its application in livestock production", J Anim Nutr, 33, pp.2527~2534 (2021)
- Cassina A, "Defective human sperm cells are associated with mitochondrial dysfunction and oxidant production", Biol Reprod, 93, pp.119~ (2015)[DOI]
- Zhong CF, "Research progress on the relationship between mitochondrial energy metabolism and weak spermatozoa", World TCM, 16, pp.666~671 (2021)[DOI]
- Longobardi V, "Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm", Theriogenology, 88, pp.1~8 (2017)[DOI]
- Guo XN, "Antioxidant effect of Radix et Rhizoma ginseng polysaccharide and its effect on the lifespan of Drosophila melanogaster", Food Sci, 34, pp.285~288 (2013)[DOI]
- Li GR, "Extraction of Codonopsis Radix polysaccharides and their effect on reactive oxygen radicals", World Chem, 08, pp.421~422 (2001)