Enhancing liquid-chilled storage and cryopreservation capacities of ram spermatozoa by supplementing the diluent with different additives
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: In the present study, we determined efficiency of incorporating caffeine, melatonin or omega-3 polyunsaturated fatty acid in the diluent on mitigating consequences of (a) liquid chilled- and (b) cryo-storage of ram spermatozoa. Methods: In the first experiment, ejaculates (n = 30) were collected from 5 adult rams and were pooled, diluted (1:10) with Tris-citric acid (base diluent) and were split into 4 aliquots assigned for: control (untreated), caffeine (0.1 mM), melatonin (0.3 mM) or omega-3 fatty acids (0.3 mM) (T0). The diluted specimens were stored at 4°C for 48 h, during which sperm physical and cytological properties were evaluated along with oxidative stress indices (T24, T48). In the second experiment, 15 ejaculates (3 per male) were pooled, diluted with glycerolized base diluent (4% glycerol, v/v) and were split corresponding to the same previous treatment groups before being processed for cryopreservation. Post-thaw physical and kinematic sperm properties were assessed by a computer-assisted sperm analysis system. Results: The results clarified superiority of both melatonin and omega-3 supplementation on maintaining (p<0.05) sperm properties, while reducing (p<0.05) lipid peroxidase reaction and enzymatic activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in preservation medium, compared to caffeine either during liquid-chilled storage or cryopreservation of spermatozoa. Conclusion: Melatonin and omega-3 are regarded efficient alternatives to caffeine when processing ram spermatozoa for application of artificial insemination or in vitro fertilization.
Abstract
Objective: In the present study, we determined efficiency of incorporating caffeine, melatonin or omega-3 polyunsaturated fatty acid in the diluent on mitigating consequences of (a) liquid chilled- and (b) cryo-storage of ram spermatozoa. Methods: In the first experiment, ejaculates (n = 30) were collected from 5 adult rams and were pooled, diluted (1:10) with Tris-citric acid (base diluent) and were split into 4 aliquots assigned for: control (untreated), caffeine (0.1 mM), melatonin (0.3 mM) or omega-3 fatty acids (0.3 mM) (T0). The diluted specimens were stored at 4°C for 48 h, during which sperm physical and cytological properties were evaluated along with oxidative stress indices (T24, T48). In the second experiment, 15 ejaculates (3 per male) were pooled, diluted with glycerolized base diluent (4% glycerol, v/v) and were split corresponding to the same previous treatment groups before being processed for cryopreservation. Post-thaw physical and kinematic sperm properties were assessed by a computer-assisted sperm analysis system. Results: The results clarified superiority of both melatonin and omega-3 supplementation on maintaining (p<0.05) sperm properties, while reducing (p<0.05) lipid peroxidase reaction and enzymatic activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in preservation medium, compared to caffeine either during liquid-chilled storage or cryopreservation of spermatozoa. Conclusion: Melatonin and omega-3 are regarded efficient alternatives to caffeine when processing ram spermatozoa for application of artificial insemination or in vitro fertilization.
참고문헌 (31)
- Baldassare H, "Advanced assisted reproduction technologies (ART) in goats", Anim Reprod Sci, 82-83, pp.255~266 (2004)[DOI]
- Câmara DR, "Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen", Small Rumin Res, 100, pp.44~49 (2011)[DOI]
- Faigl V, "Artificial insemination of small ruminants-a review", Acta Vet Hung, 60, pp.115~129 (2012)[DOI]
- Peña FJ, "Mitochondria in mammalian sperm physiology and pathology : a review", Reprod Domest Anim, 44, pp.345~349 (2009)[DOI]
- Ashrafi I, "Protective effect of melatonin on sperm motility parameters on liquid storage of ram semen at 5°C", Afr J Biotechnol, 10, pp.6670~6674 (2011)
- Agarwal A, "Role of reactive oxygen species in the pathophysiology of human reproduction", Fertil Steril, 79, pp.829~843 (2003)[DOI]
- Alvarez JG, "Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa", Mol Reprod Dev, 42, pp.334~346 (1995)[DOI]
- Soren S, "Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions", Turk J Vet Anim Sci, 40, pp.797~802 (2016)[DOI]
- Mostafa T, "Seminal reactive oxygen species‐antioxidant relationship in fertile males with and without varicocele", Andrologia, 41, pp.125~129 (2009)[DOI]
- El-Raey MR, "Evidences for the role of melatonin as a Protective additive during buffalo semen freezing", Am J Anim Vet Sci, 9, pp.252~262 (2014)[DOI]
- El-Shahat KH, "Effect of heparin, caffeine and calcium ionophore A23187 on in vitro induction of the acrosome reaction of fresh ram spermatozoa", Asian Pac J Reprod, 5, pp.148~155 (2016)[DOI]
- Kaka A, "Alpha-linolenic acid supplementation in tris extender can improve frozen–thawed bull semen quality", Reprod Domest Anim, 50, pp.29~33 (2015)[DOI]
- NRC, "Nutrient requirement of small ruminants: sheep, goats, cervids, and new world camelids" (2007), National Academies Press
- Rateb SA, "Influence of omega-3 incorporation in sperm preservation medium on physical and kinematic properties of chilled and cryopreserved ram spermatozoa", Reprod Domest Anim, 53, pp.1506~1516 (2018)[DOI]
- Abd El-Hamid IS, "Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen", Reprod Domest Anim, 54, pp.225~233 (2019)[DOI]
- Khalifa MA, "Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin", Int J Anim Res, 1, pp.17~ (2017)[DOI]
- Mosaferi S, "Biophysical and biochemical characteristics of bactrian camel semen collected by artificial vagina", Theriogenology, 63, pp.92~101 (2005)[DOI]
- "Laboratory manual for the examination and processing of human semen" (2010)[URL]
- IBM Corp, "IBM SPSS Statistics for Windows, Version 22.0", IBM Corp, ~ (2013)
- Lessard C, "Cryopreservation alters the levels of the bull sperm surface protein P25b", J Androl, 21, pp.700~707 (2000)[DOI]
- Wongtawan T, "Fertility after deep intra-uterine artificial insemination of concentrated low-volume boar semen doses", Theriogenology, 65, pp.773~787 (2006)[DOI]
- Bailey JL, "Semen cryopreservation : successes and persistent problems in farm species", Can J Anim Sci, 83, pp.393~401 (2003)[DOI]
- Špaleková EA, "Effect of caffeine on functions of cooling-stored ram sperm in vitro", Acta Vet Brno, 83, pp.19~25 (2014)[DOI]
- Shukla MK, "Caffeine as a semen additive to improve Murrah buffalo(Bubalus bubalis)semen cryopreservation", Buffalo Bulletin, 33, pp.32~36 (2014)
- El-Bahrawy KA, "The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa", Int J Vet Sci Med, 5, pp.121~127 (2017)[DOI]
- Suarez SS, "Control of hyperactivation in sperm", Hum Reprod Update, 14, pp.647~657 (2008)[DOI]
- Singh P, "Effect of caffeine, cAMP and cattle seminal plasma on freezability of buffalo bull semen", Asian-Australas J Anim Sci, 13, pp.901~905 (2000)[DOI]
- Kang JT, "Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells", J Pineal Res, 46, pp.22~28 (2009)[DOI]
- Thatcher WW, "Using fats and fatty acids to enhance reproductive performance", Proceeding of the 5th Mid-Atlantic Nutrition Conference, pp.116~129 (2007), University of Maryland
- Robinson JJ, "Nutrition and fertility in ruminant livestock", Anim Feed Sci Technol, 126, pp.259~276 (2006)[DOI]
- Kiernan M, "The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function", Reprod Fertil Dev, 25, pp.947~954 (2012)[DOI]