Polydatin 처리에 의한 사람치수줄기세포(hDPSCs)의 증식과 분화촉진 효과
Polydatin-induced Proliferation and Differentiation in Human Dental Pulp Stem Cells
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
폴리다틴(Polydatin; PD)는 레스베라트롤(Resveratrol; RSV)의 전구체로 항염증 및 뼈형성을 촉진하는 것으로 알려진 천연물질이다. 사람치수줄기세포(Human dental pulp stem cells; hDPSCs)는 치수 조직 내에 존재하는 성체 줄기세포로 골수중간엽줄기세포보다 높은 증식 능력을 가진다. 또한, 다른 조직의 줄기세포에 비해 비교적 쉽게 채취할 수 있으며, 치아 및 신경 재생에 적합한 특징을 가지고 있다. 본 연구에서는 hDPSCs의 분화와 관련하여 폴리다틴이 hDPSCs의 증식을 촉진하고 뼈분화 형성에 관여하는 단백질의 발현을 조절함으로써 치수 및 치아 조직 재생에 기여함을 밝혀냈다. 폴리다틴을 hDPSCs에 처리한 후 세포의 생존율, 증식율을 평가하였으며 치아분화에 관여하는 단백질의 발현을 확인하였다. 또한 세포 주기 단백질과의 관련성을 평가하여 폴리다틴이 hDPSCs에서 관련 분화 마커들의 활성화와 치아 혹은 뼈 분화를 촉진하는 과정을 평가하였다. 결론적으로 본 연구는 천연 물질인 폴리다틴이 hDPSCs의 세포주기와 증식을 촉진하고 치아의 광물질화를 촉진하는 물질로서 높은 가치를 가진다는 것을 입증하였다.
Abstract
Polydatin (PD) is a natural compound and precursor of resveratrol (RSV), with known anti-inflammatory and osteogenic properties. Human dental pulp stem cells (hDPSCs), adult stem cells in the dental pulp tissue, exhibit a higher proliferation capacity than bone marrow mesenchymal stem cells and are relatively easy to obtain. Their characteristics make them suitable for tooth and nerve regeneration applications. This study examined the effects of polydatin on hDPSCs differentiation, demonstrating that PD enhances cell proliferation and regulates the expression of proteins associated with osteogenic differentiation, thus facilitating pulp and dental tissue regeneration. After PD treatment of hDPSCs, we assessed cell viability, proliferation rates, and the expression of proteins associated with osteogenic or odontogenic differentiation. Furthermore, we evaluated the relationship between PD and cell cycle proteins to understand how PD activates relevant markers and promotes osteogenic or odontogenic differentiation in hDPSCs. In conclusion, this study demonstrated that polydatin, as a natural compound, holds substantial value as an agent that promotes cell cycle, cell proliferation, and mineralization in hDPSCs.
참고문헌 (28)
- Vakili Ojarood, M., "Human dental pulp stem cells for burn wound healing : Potential therapeutic applications", Burns, 50(8), pp.2124~2126 (2024)[DOI]
- Takaoka, S., "Sequencing-based study of neural induction of human dental pulp stem cells", Hum Cell, 37(6), pp.1638~1648 (2024)[DOI]
- Alfahlawy, A., "Effect of three different root canal sealants on human dental pulp stem cells", Sci Rep, 14(1), pp.23937~ (2024)[DOI]
- Ali, M., "Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt", beta-catenin signaling, 2019, pp.96, 594~604
- Baek, H. S., "Chios gum mastic enhance the proliferation and odontogenic differentiation of human dental pulp stem cells", Korean J Physiol Pharmacol, 28(5), pp.423~433 (2024)[DOI]
- Matacchione, G., "Curcumin, Polydatin and Quercetin Synergistic Activity Protects from High-Glucose-Induced Inflammation and Oxidative Stress", Antioxidants(Basel), 11(6), pp.1037~ (2022)[DOI]
- Giordani, C., "Pro-Osteogenic and Anti-Inflammatory Synergistic Effect of Orthosilicic Acid, Vitamin K2, Curcumin, Polydatin and Quercetin Combination in Young and Senescent Bone Marrow-Derived Mesenchymal Stromal Cells", Int J Mol Sci, 24(10), pp.8820~ (2023)[DOI]
- Luo, J., "Pharmacological effects of polydatin in the treatment of metabolic diseases : A review", Phytomedicine, 102, pp.154~161 (2022)[DOI]
- Li, J., "Natural Compounds, Optimal Combination of Brusatol and Polydatin Promote Anti-Tumor Effect in Breast Cancer by Targeting Nrf2 Signaling Pathway", Int J Mol Sci, 24(9), pp.8265~ (2023)[DOI]
- Liu, M., "Polydatin down-regulates the phosphorylation level of STAT3 and induces pyroptosis in triple-negative breast cancer mice with a high-fat diet", Ann Transl Med, 10(4), pp.173~ (2022)[DOI]
- Serafino, A., "The Synergistic Combination of Curcumin and Polydatin Improves Temozolomide Efficacy on Glioblastoma Cells", Int J Mol Sci, 25(19), pp.10572~ (2024)[DOI]
- Al-Ateeq, R., "Effect of polydatin on the viability and odontogenic differentiation of human dental pulp stem cells : An in-vitro study", J Dent Sci, 19(4), pp.2332~2340 (2024)[DOI]
- Maccaferri, M., "Human dental pulp stem cells modulate pro-inflammatory macrophages both through cell-to-cell contact and paracrine signaling", Front Immunol, 15, pp.1440974~ (2024)[DOI]
- Xu, W., "An Investigation about Gene Modules Associated with hDPSC Differentiation for Adolescents", Stem Cells Int, eCollection, pp.8913287~ (2019)[DOI]
- Shah, W., "Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions", Sci Rep, 13(1), pp.16550~ (2023)[DOI]
- Lama, S., "Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation", Pharmaceuticals(Basel), 15(6), pp.727~ (2022)[DOI]
- Zhou, J., "Application of beta-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol", Molecules, 27(5), pp.1514~ (2022)[DOI]
- Boreak, N., "Dose-Dependent Effect of Cordycepin on Viability, Proliferation, Cell Cycle, and Migration in Dental Pulp Stem Cells", J Pers Med, 11(8), pp.718~ (2021)[DOI]
- Fan, W., "Aspirin inhibits the proliferation of synovium-derived mesenchymal stem cells by arresting the cell cycle in the G0", G1 phase, 2017, pp.9(11), 5056~5062
- Liu, L., "The cell cycle in stem cell proliferation, pluripotency and differentiation", Nat Cell Biol, 21(9), pp.1060~1067 (2019)[DOI]
- Shen, S, "An ALP-responsive, anionic iridium complex for specific recognition of osteosarcoma cells", Dalton Trans, 53(20), pp.8633~8641 (2024)[DOI]
- Tyagi, K., "Alkaline phosphatase(ALP)activatable small molecule-based prodrugs for cancer theranostics", Org Biomol Chem, 21(21), pp.4455~4464 (2023)[DOI]
- Chicco, D., "Computational intelligence identifies alkaline phosphatase (ALP), alpha-fetoprotein (AFP), and hemoglobin levels as most predictive survival factors for hepatocellular carcinoma", Health Informatics J, 27(1), pp.1460458220984205~ (2021)[DOI]
- Ma, L., "Combined Effects of Proton Radiation and Simulated Microgravity on the Cell Viability and ALP Activity of Murine Osteoblast Cells", Front Public Health, 9, pp.759236~ (2021)[DOI]
- Ahmad, A. R., "Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells", Biotechnol Rep (Amst), 23, pp.e00348~ (2019)[DOI]
- Kongkiatkamon, S., "Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells", Molecules, 26(22), pp.6756~ (2021)[DOI]
- Spagnuolo, G., "Regeneration of dentin-pulp complex : Effect of calcium-based materials on hDPSCs differentiation and gene expression", Dent Mater, 39(5), pp.485~491 (2023)[DOI]
- Szczodra, A., "Boron substitution in silicate bioactive glass scaffolds to enhance bone differentiation and regeneration", Acta Biomater, 186, pp.489~506 (2024)[DOI]