Morphology and Molecular Phylogeny of Raillietina spp. (Cestoda: Cyclophyllidea: Davaineidae) from Domestic Chickens in Thailand
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.
Abstract
Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.
참고문헌 (21)
- Schmidt GD, "Handbook of Tapeworm Identification", pp.252~266 (1986), CRC Press, Inc
- Butboonchoo P, "Occurrence and HAT-RAPD analysis of gastrointestinal helminths in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand", Saudi J Biol Sci (2015)
- Permin A, "The Epidemiology, diagnosis and control of poultry parasites. A FAO Handbook", pp.36~39 (2003), Food and Agriculture Organization of the United Nations
- Hofstad MS, "Diseases of Poultry" (1984), Iowa State University Press
- Alenyorege B, "Termites as intermediate hosts for poultry worms", J Vet Adv, 1, pp.16~23 (2011)
- Yamaguti S, "Systema Helminthum. Vol. II. The Cestodes of Vertebrates", pp.1~860 (1959), Interscience Publishers Inc
- Khalil LF, "Keys to the Cestode Parasites of Vertebrates", pp.1~424 (2006), International Institute of Parasitology
- Littlewood DTJ, "In search of mitochondrial marker for resolving the phylogeny of cyclophyllidean tapeworms (Platyhelminths, Cestode) – a test study with Davaineidae", Acta Parasitol, 53, pp.133~144 (2008)
- Okamoto M, "Phylogenetic relationship of three hymenolepidid species inferred from nuclear ribosomal and mitochondrial DNA sequences", Parasitology, 115, pp.661~666 (1997)
- Wickstrom LM, "Molecular phylogeny and systematics of anoplocephaline cestodes in rodents and lagomorphs", Syst Parasitol, 62, pp.83~99 (2005)
- Lavikainen A, "A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data", Parasitology, 135, pp.1457~1467 (2008)
- Sawada I, "On the genus Raillietina Fuhrmann, 1920 (I)", J Nara Gakugei Univ (Natural Science), 12, pp.19~36 (1964)
- Sawada I, "On the genus Raillietina Fuhrmann 1920 (II)", J Nara Gakugei Univ (Natural Science), 13, pp.5~38 (1965)
- Ilie MS, "The electron-microscopic characterization of some helminths found in birds", Lucrari Stiintifice Med Vet, 41, pp.402~410 (2008)
- Lalchhandama K, "On the structure of Raillietina echinobothrida, the tapeworm of domestic fowl", Sci Vis, (4), pp.174~182 (2009)
- Kunda S, "vitro screening for cestocidal activity of three species of Cassis plants against the tapeworm Raillietina tetragona", J Helminthol, 87, pp.154~159 (2013)
- Xiao N, "Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China", Int J Parasitol, 35, pp.693~701 (2005)
- Malsawmtluangi C, "Morphological and molecular identification of the metacestode parasitizing the liver of rodent hosts in bamboo growing areas of Mizoram, northeast India", Bioinformation, 7, pp.393~399 (2011)
- Ghobashy MA, "Molecular characterization of Raillietina (r.) spp. Ortlepp, 1938 (Cestode: Cyclophyllidea: Davaineidae) infecting domestic and wild bird (Columba livia and Columba livia domestica)", World J Zool, 10, pp.136~141 (2015)
- Ramnath DB, "characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on internal transcribed spacer 2 region", J Parasit Dis, 38, pp.22~26 (2014)
- O’ Callaghan MG, "Species of Raillietina Fuhrmann, 1920 (Cestoda: Davaineidae) from the emu, Dromaius novaehollandiae", Trans R Soc S Aust, 124, pp.105~116 (2000)