Assessment of Adipocyte Differentiation and Maturation-related Gene Expression in the Epididymal Fat of Estrogen Receptor α Knockout (ERαKO) Mouse during Postnatal Development Period
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The absence of functional estrogen receptor α (Esr1) results in an overgrowth of the epididymal fat, as observed in estrogen receptor α knockout (ERαKO) mouse. The present research was aimed to evaluate expression of various molecules associated with adipocyte differentiation and maturation in the epididymal fat of ERαKO mouse at several postnatal ages by using quantitative real-time polymerase chain reaction. The highest transcript levels of all molecules were detected at 12 months of postnatal age, except leptin which the mRNA level was increased at 5 months of age and was unchanged until 12 months of age. The expression levels of CCAAT enhancer binding protein (Cebp) alpha, androgen receptor, and lipoprotein lipase were decreased at 5 months of age but increased at about 8 months of age. The mRNA levels of Cebp gamma and sterol regulatory element binding transcription factor 1 remained steady until 8 months of age. Continuous increases of transcript levels during postnatal period were found in Cebp beta, estrogen receptor (ER) beta, fatty acid binding protein 4, and delta like non-canonical Notch ligand 1. The increases of peroxisome proliferator-activated receptor gamma and adiponectin mRNA levels were detected as early as 8 months of age. The levels of fatty acid synthase and resistin transcript at 5 and 8 months of age were lower than that at 2 months of age. These findings show the aberrant expression patterns of genes related to adipocyte differentiation and maturation in the postnatal epididymal fat pad by the disruption of ER alpha function.
Abstract
The absence of functional estrogen receptor α (Esr1) results in an overgrowth of the epididymal fat, as observed in estrogen receptor α knockout (ERαKO) mouse. The present research was aimed to evaluate expression of various molecules associated with adipocyte differentiation and maturation in the epididymal fat of ERαKO mouse at several postnatal ages by using quantitative real-time polymerase chain reaction. The highest transcript levels of all molecules were detected at 12 months of postnatal age, except leptin which the mRNA level was increased at 5 months of age and was unchanged until 12 months of age. The expression levels of CCAAT enhancer binding protein (Cebp) alpha, androgen receptor, and lipoprotein lipase were decreased at 5 months of age but increased at about 8 months of age. The mRNA levels of Cebp gamma and sterol regulatory element binding transcription factor 1 remained steady until 8 months of age. Continuous increases of transcript levels during postnatal period were found in Cebp beta, estrogen receptor (ER) beta, fatty acid binding protein 4, and delta like non-canonical Notch ligand 1. The increases of peroxisome proliferator-activated receptor gamma and adiponectin mRNA levels were detected as early as 8 months of age. The levels of fatty acid synthase and resistin transcript at 5 and 8 months of age were lower than that at 2 months of age. These findings show the aberrant expression patterns of genes related to adipocyte differentiation and maturation in the postnatal epididymal fat pad by the disruption of ER alpha function.
참고문헌 (21)
- Berry DC, "The developmental origin of adipose tissue", Development, 140, pp.3939~3949 (2013)
- Cleary MP, "A multifactor analysis of growth in the rat epididymal fat pad", J Nutr, 107, pp.1969~1974 (1977)
- Cooke PS, "Role of estrogens in adipocyte development and function", Exp Biol Med, 229, pp.1127~1135 (2004)
- Dieudonne MN, "Androgen receptors in cultured rat adipose precursor cells during proliferation and differentiation : regional specificities and regulation by testosterone", Endocrine, 3, pp.537~541 (1995)
- Fasshauer M, "Adipokines in health and disease", Trends Pharmacol Sci, 36, pp.461~470 (2015)
- Gieske MC, "Pituitary gonadotroph estrogen receptor-alpha is necessary for fertility in females", Endocrinology, 149, pp.20~27 (2008)
- Gray JM, "Cytoplasmic estrogen, but not progestin, binding sites in male rat adipose tissues", Am J Physiol, 239, pp.E237~E241 (1980)
- Han J, "The spatiotemporal development of adipose tissue", Development, 138, pp.5027~5037 (2011)
- Hausman DB, "The biology of white adipocyte proliferation", Obes Rev, 2, pp.239~254 (2001)
- Heine PA, "Increased adipose tissue in male and female estrogen receptor-α knockout mice", Proc Natl Acad Sci USA, 97, pp.12729~12734 (2000)
- Jones MEE, "Aromatase-deficient(ArKO)mice have a phenotype of increased adiposity", Proc Natl Acad Sci USA, 97, pp.12735~12740 (2000)
- Knittle JL, "Effect of early nutrition on the development of rat epididymal fat pads : cellularity and metabolism", J Clin Invest, 47, pp.2091~2098 (1968)
- Kõressaar T, "Primer3_ masker : integrating masking of template sequence with primer design software", Bioinformatics, 34, pp.1937~1938 (2018)
- 이기호, "Postnatal Expressional Patterns of Adipose-Associated Molecules in the Mouse Proximal Epididymal Fat", 발생과 생식, 23(4), pp.313~322 (2019), 한국발생생물학회
- 이기호, "Expression of Adipocyte-Associated Genes in the Mouse Tail Epididymal Fat at Different Postnatal Ages", 발생과 생식, 24(3), pp.167~176 (2020), 한국발생생물학회
- 이기호, "Differential Expression of Adipocyte-Related Molecules in the Distal Epididymal Fat of Mouse during Postnatal Period", 발생과 생식, 23(3), pp.213~221 (2019), 한국발생생물학회
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative pcr and the 2−ΔΔCt method", Methods, 25, pp.402~408 (2001)
- Mareno-Navarrete JM, "Adipose Tissue Biology", pp.17~38 (2012), Springer
- Metz L, "Nutritional and exercise interventions variably affect estrogen receptor expression in the adipose tissue of male rats", Nutr Res, 36, pp.280~289 (2016)
- Niemelä S, "Topics in Tissue Engineering, Vol. 4", pp.1~26 (2008), Oulu University
- Tirard J, "A novel inhibitory protein in adipose tissue, the aldo-keto reductase AKR1B7 : its role in adipogenesis", Endocrinology, 148, pp.1996~2005 (2007)