Expression of Adipocyte-Associated Genes in the Mouse Tail Epididymal Fat at Different Postnatal Ages
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.
Abstract
The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.
참고문헌 (20)
- Berry DC, "The developmental origins of adipose tissue", Development, 140, pp.3939~3949 (2013)
- Björntorp P, "Quantitation of different cells in the epididymal fat pad of the rat", J Lipid Res, 20, pp.97~106 (1979)
- Chu Y, "Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior", Endocrinology, 151, pp.5669~5679 (2010)
- Cleary MP, "A multi-factor analysis of growth in the rat epididymal fat pad", J Nutr, 107, pp.1969~1974 (1977)
- Gruen R, "Development of basal lipolysis and fat cell size in the epididymal fat pad of normal rats", Metabolism, 29, pp.246~253 (1980)
- Haiming C, "Adipocytokines in obesity and metabolic disease", J Endocrinol, 220, pp.T47~T59 (2014)
- Han J, "The spatiotemporal development of adipose tissue", Development, 138, pp.5027~5037 (2011)
- Hausman DB, "The biology of white adipocyte proliferation", Obes Rev, 2, pp.239~254 (2001)
- Kletzien RF, "Adipocyte fatty acid-binding protein : Regulation of gene expression in vivo and in vitro by an insulin-sensitizing agent", Mol Pharmacol, 42, pp.558~562 (1992)
- 이기호, "Postnatal Expressional Patterns of Adipose-Associated Molecules in the Mouse Proximal Epididymal Fat", 발생과 생식, 23(4), pp.313~322 (2019), 한국발생생물학회
- 이기호, "Expressional Patterns of Adipocyte-Associated Molecules in the Rat Epididymal Fat during Postnatal Development Period", 발생과 생식, 22(4), pp.351~360 (2018), 한국발생생물학회
- 이기호, "Differential Expression of Adipocyte-Related Molecules in the Distal Epididymal Fat of Mouse during Postnatal Period", 발생과 생식, 23(3), pp.213~221 (2019), 한국발생생물학회
- Pond CM, "Physiological specialisation of adipose tissue", Prog Lipid Res, 38, pp.225~248 (1999)
- Proença ARG, "New concepts in white adipose tissue physiology", Braz J Med Biol Res, 47, pp.192~205 (2014)
- Roumaud P, "Roles of leptin, adiponectin and resistin in the transcriptional regulation of steroidogenic genes contributing to decreased Leydig cells function in obesity", Horm Mol Biol Clin Investig, 24, pp.25~45 (2015)
- Singh A, "Adiponectin and chemerin : Contrary adipokines in regulating reproduction and metabolic disorders", Reprod Sci, 25, pp.1462~1473 (2018)
- Tamori Y, "Role of peroxisome proliferator-activated receptor-γ in maintenance of the characteristics of mature 3T3-L1 adipocytes", Diabetes, 51, pp.2045~2055 (2002)
- Tirard J, "A novel inhibitory protein in adipose tissue, the aldo-keto reductase AKR1B7 : Its role in adipogenesis", Endocrinology, 148, pp.1996~2005 (2007)
- Wang Y, "Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9", Cell Metab, 9, pp.287~302 (2009)
- Zhang J, "Review of the role of leptin in the regulation of male reproductive function", Andrologia, 50, pp.e12965~ (2018)