Weissella cibaria CMU suppresses mgl gene expression and enzyme activity associated with bad breath
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The oral care probiotic strain Weissella cibaria CMU (oraCMU) inhibits volatile sulphur compounds associated with halitosis, presumably by inhibiting the growth of associated oral pathogens. In the present study, we investigated whether oraCMU inhibits the production of these compounds by suppressing the expression of mgl . This gene encodes L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) and is involved in the production of methyl mercaptan (CH 3 SH) by Porphyromonas gingivalis . Therefore, we specifically investigated the effects of oraCMU on the growth, CH 3 SH production, METase activity, and mgl expression of P. gingivalis . The minimum inhibitory concentrations of cell-free supernatant and secreted proteins from oraCMU were 125 mg/mL and 800 μg/mL, respectively. At sub-minimum inhibitory concentration levels, these metabolites inhibited CH 3 SH production, but they also reduced P. gingivalis viability. Only heat-killed oraCMU decreased CH 3 SH production without affecting P. gingivalis viability. Heat-killed oraCMU also inhibited METase activity toward L-methionine and mgl mRNA expression ( p < 0.05). In summary, we demonstrated the inhibition of volatile sulphur compounds via the antimicrobial action of oraCMU and, for the first time, the inhibition of such compounds by heat-killed oraCMU, which occurred at the molecular level.
Abstract
The oral care probiotic strain Weissella cibaria CMU (oraCMU) inhibits volatile sulphur compounds associated with halitosis, presumably by inhibiting the growth of associated oral pathogens. In the present study, we investigated whether oraCMU inhibits the production of these compounds by suppressing the expression of mgl . This gene encodes L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) and is involved in the production of methyl mercaptan (CH 3 SH) by Porphyromonas gingivalis . Therefore, we specifically investigated the effects of oraCMU on the growth, CH 3 SH production, METase activity, and mgl expression of P. gingivalis . The minimum inhibitory concentrations of cell-free supernatant and secreted proteins from oraCMU were 125 mg/mL and 800 μg/mL, respectively. At sub-minimum inhibitory concentration levels, these metabolites inhibited CH 3 SH production, but they also reduced P. gingivalis viability. Only heat-killed oraCMU decreased CH 3 SH production without affecting P. gingivalis viability. Heat-killed oraCMU also inhibited METase activity toward L-methionine and mgl mRNA expression ( p < 0.05). In summary, we demonstrated the inhibition of volatile sulphur compounds via the antimicrobial action of oraCMU and, for the first time, the inhibition of such compounds by heat-killed oraCMU, which occurred at the molecular level.
참고문헌 (33)
- I. Kleinberg, "Oral Malodor", Critical Reviews in Oral Biology & Medicine, 1(4), pp.247~259 (2016), SAGE Publications[DOI]
- Sten Persson, "The formation of hydrogen sulfide and methyl mercaptan by oral bacteria", Oral Microbiology and Immunology, 5(4), pp.195~201 (1990), Wiley[DOI]
- ERIKA H. DE BOEVER, "ASSESSING THE CONTRIBUTION OF ANAEROBIC MICROFLORA OF THE TONGUE TO ORAL MALODOR", The Journal of the American Dental Association, 126(10), pp.1384~1393 (1995), Elsevier BV[DOI]
- Yoshio Nakano, "Correlation between oral malodor and periodontal bacteria", Microbes and Infection, 4(6), pp.679~683 (2002), Elsevier BV[DOI]
- Joseph Tonzetich, "Production and Origin of Oral Malodor: A Review of Mechanisms and Methods of Analysis", Journal of Periodontology, 48(1), pp.13~20 (1977), Wiley[DOI]
- M. Yoshimura, "Formation of Methyl Mercaptan from L-Methionine by Porphyromonas gingivalis", Infection and Immunity, 68(12), pp.6912~6916 (2000), American Society for Microbiology[DOI]
- Mariela Bustamante, "Probiotics as an Adjunct Therapy for the Treatment of Halitosis, Dental Caries and Periodontitis", Probiotics and Antimicrobial Proteins (2019), Springer Science and Business Media LLC[DOI]
- Nao Suzuki, "Induction and inhibition of oral malodor", Molecular Oral Microbiology, 34(3), pp.85~96 (2019), Wiley[DOI]
- L. Masdea, "Antimicrobial activity of Streptococcus salivarius K12 on bacteria involved in oral malodour", Archives of Oral Biology, 57(8), pp.1041~1047 (2012), Elsevier BV[DOI]
- Nao Suzuki, "Lactobacillus salivarius WB21–containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial", Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 117(4), pp.462~470 (2014), Elsevier BV[DOI]
- Mette K. Keller, "Effect of chewing gums containing the probiotic bacterium Lactobacillus reuteri on oral malodour", Acta Odontologica Scandinavica, 70(3), pp.246~250 (2011), Informa UK Limited[DOI]
- Mi-Sun Kang, "Inhibitory effect of Weissella cibaria isolates on the production of volatile sulphur compounds", Journal of Clinical Periodontology, 33(3), pp.226~232 (2006), Wiley[DOI]
- Hye-Jin Jang, "Comparative Study on the Characteristics of Weissella cibaria CMU and Probiotic Strains for Oral Care", Molecules, 21(12), pp.E1752~ (2016), MDPI AG[DOI]
- Ulrich Schillinger, "Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples.", International Journal of Systematic and Evolutionary Microbiology, 52(Pt 1), pp.141~148 (2002), Microbiology Society[DOI]
- Bo Kyoung Kang, "Red pepper powder is a crucial factor that influences the ontogeny of Weissella cibaria during kimchi fermentation", Scientific Reports, 6(1), pp.28232~ (2016), Springer Science and Business Media LLC[DOI]
- Borja Sánchez, "A method for the identification of proteins secreted by lactic acid bacteria grown in complex media", FEMS Microbiology Letters, 295(2), pp.226~229 (2009), Oxford University Press (OUP)[DOI]
- Hae-Soon Lim, "Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic Weissella cibaria, CMU", Molecules, 23(8), pp.1984~ (2018), MDPI AG[DOI]
- M. M. Tunney, "Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa", Antimicrobial Agents and Chemotherapy, 48(5), pp.1879~1881 (2004), American Society for Microbiology[DOI]
- George L. Ellman, "Tissue sulfhydryl groups", Archives of Biochemistry and Biophysics, 82(1), pp.70~77 (1959), Elsevier BV[DOI]
- X. Xu, "Tea catechin EGCg suppresses the mgl gene associated with halitosis", Journal of Dental Research, 89(11), pp.1304~1308 (2010), SAGE Publications[DOI]
- Claudia Nonnenmacher, "Quantitative detection of periodontopathogens by real-time PCR", Journal of Microbiological Methods, 59(1), pp.117~125 (2004), Elsevier BV[DOI]
- 도경효, "Weissella cibaria CMU 경구투여가 비글의 구취 저하에 미치는 효과", 대한수의학회지, 58(2), pp.87~94 (2018), 대한수의학회[DOI]
- Kyung-Hyo Do, "Effects of Weissella cibaria CMU on halitosis and calculus, plaque, and gingivitis indices in beagles", Journal of Veterinary Dentistry, 36(2), pp.135~142 (2019), SAGE Publications[DOI]
- Hae-Ryoung Park, "Clinical studies on the dental caries prevention effects of the ability of Weissella cibaria CMU to adhere to the oral cavity", Indian Journal of Public Health Research & Development, 9(9), pp.1163~1169 (2018), Diva Enterprises Private Limited[DOI]
- 강미선, "Effect of Weissella cibaria on Fusobacterium nucleatum-induced Interleukin-6 and Interleukin-8 Production in KB Cells", Journal of Bacteriology and Virology, 41(1), pp.9~18 (2011), 대한미생물학회
- 유형석, "Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells", Journal of Microbiology and Biotechnology, 29(7), pp.1022~1032 (2019), 한국미생물·생명공학회[DOI]
- 홍이판, "Lipoteichoic Acid Isolated from Weissella cibaria Increases Cytokine Production in Human Monocyte-Like THP-1 Cells and Mouse Splenocytes", Journal of Microbiology and Biotechnology, 26(7), pp.1198~1205 (2016), 한국미생물·생명공학회[DOI]
- Hyun Lee, "Butanol extracts of Asparagus?cochinchinensis fermented with Weissella?cibaria inhibit iNOS‑mediated COX‑2 induction pathway and inflammatory cytokines in LPS‑stimulated RAW264.7 macrophage cells", Experimental and Therapeutic Medicine, 14, pp.4986~4994 (2017), Spandidos Publications[DOI]
- 곽신혜, "Cancer Preventive Potential of Kimchi Lactic Acid Bacteria (Weissella cibaria, Lactobacillus plantarum)", 대한암예방학회지, 19(4), pp.253~258 (2014), 대한암예방학회[DOI]
- Rwivoo Baruah, "Functional food applications of dextran from Weissella cibaria RBA12 from pummelo (Citrus maxima )", International Journal of Food Microbiology, 242, pp.124~131 (2017), Elsevier BV[DOI]
- Mi-Sun Kang, "Safety Evaluation of Oral Care Probiotics Weissella cibaria CMU and CMS1 by Phenotypic and Genotypic Analysis", International Journal of Molecular Sciences, 20(11), pp.2693~ (2019), MDPI AG[DOI]
- K Ouhara, "The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan", Oral Diseases, 21(5), pp.626~633 (2015), Wiley[DOI]
- N Fujiwara, "Novel reuterin-related compounds suppress odour by periodontopathic bacteria", Oral Diseases, 23(4), pp.492~497 (2017), Wiley[DOI]