A novel recombinant BCG vaccine encoding eimeria tenella rhomboid and chicken IL-2 induces protective immunity against coccidiosis.
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4(+) and CD8(+) cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
Abstract
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4(+) and CD8(+) cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.
참고문헌 (27)
- Subramanian BM, "Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds", Vaccine, 26, pp.3489~3496 (2008)
- Chapman HD, "Sustainable coccidiosis control in poultry production : the role of live vaccines", Int J Parasitol, 32, pp.617~629 (2002)
- Lillehoj HS, "Avian coccidiosis. A review of acquired intestinal immunity and vaccination strategies", Avian Dis, 44, pp.408~425 (2000)
- Vermeulen AN, "Control of coccidiosis in chickens by vaccination", Vet Parasitol, 100, pp.13~20 (2001)
- Ding X, "Protective immunity against Eimeria acervulina following in ovo immunization with a recombinant subunit vaccine and cytokine genes", Infect Immun, 72, pp.6939~6944 (2004)
- Laurent F, "Analysis of chicken mucosal immune response to Eimeria tenella and Eimeria maxima infection by quantitative reverse transcription-PCR", Infect Immun, 69, pp.2527~2534 (2001)
- Shen H, "Novel recombinant BCG coexpressing Ag85B, ESAT-6 and mouse TNF-α induces significantly enhanced cellular immune and antibody responses in C57BL", 6 mice, 2010, pp.54, 435~441
- Sundick RS, "A cloned chicken lymphokine homologous to both mammalian IL-2 and IL-15", J Immunol, 159, pp.720~725 (1997)
- Lillehoj HS, "Molecular, cellular, and functional characterization of chicken cytokines homologous to mammalian IL-15 and IL-2", Vet Immunol Immunopathol, 82, pp.229~244 (2001)
- Min W, "Adjuvant effects of IL-1β, IL-2, IL-8, IL-15, IFN-α, IFN-γ, TGF-β4 and lymphotactin on DNA vaccination against Eimeria acervulina", Vaccine, 20, pp.267~274 (2001)
- Stepaniak JA, "Production and in vitro characterization of recombinant chicken interleukin-2", J Interferon Cytokine Res, 19, pp.515~526 (1999)
- Wang Q, "Protective immunity of recombinant Mycobacterium bovis BCG expressing rhomboid gene against Eimeria tenella challenge", Vet Parasitol, 160, pp.198~203 (2009)
- Yang G, "Eimeria tenella : construction of a recombinant fowlpox virus expressing rhomboid gene and its protective efficacy against homologous infection", Exp Parasitol, 119, pp.30~36 (2008)
- Xu Q, "Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis", Vet Parasitol, 156, pp.319~323 (2008)
- Lillehoj HS, "Resistance to intestinal coccidiosis following DNA immunization with the cloned 3-1E Eimeria gene plus IL-2, IL-15, and IFN-γ", Avian Dis, 49, pp.112~117 (2005)
- Pasquini S, "Cytokines and costimulatory molecules as genetic adjuvants", Immunol Cell Biol, 75, pp.397~401 (1997)
- Li J, "Eimeria tenella : cloning of a novel Eimeria tenella cDNA encoding a protein related to rhomboid family from F2 hybrid strain", Exp Parasitol, 113, pp.215~220 (2006)
- Kumar D, "Genetic rearrangements leading to disruption of heterologous gene expression in mycobacteria : an observation with Escherichia coli beta-galactosidase in Mycobacterium smegmatis and its implication in vaccine development", Vaccine, 16, pp.1212~1215 (1998)
- Wang Q, "Construction of EGFP-tagged rBCG of E. tenella and distribution in chickens", Sci China C Life Sci, 52, pp.278~283 (2009)
- Johnson J, "Anticoccidial drug : lesion scoring techniques in battery and floor-pen experiments with chickens", Exp Parasitol, 28, pp.30~36 (1970)
- Kogut M, "Differential effects of age on chicken heterophil functional activation by recombinant chicken interleukin-2", Dev Comp Immunol, 26, pp.817~830 (2002)
- Klasing KC, "Avian leukocytic cytokines", Poult Sci, 73, pp.1035~1043 (1994)
- Brossier F, "A spatially localized rhomboid protease cleaves cell surface adhesins essential for invasion by Toxoplasma", Proc Natl Acad Sci USA, 102, pp.4146~4151 (2005)
- Dowse TJ, "Rhomboid-like proteins in Apicomplexa : phylogeny and nomenclature", Trends Parasitol, 21, pp.254~258 (2005)
- Bastos RG, "Recombinant Mycobacterium bovis BCG", Vaccine, 27, pp.6495~6503 (2009)
- Gicquel B, "BCG as a vector for the construction of multivalent recombinant vaccines", Biologicals, 23, pp.113~118 (1995)
- Lillehoj HS, "Avian gut-associated lymphoid tissues and intestinal immune responses to Eimeria parasites", Clin Microbiol Rev, 9, pp.349~360 (1996)