본문 바로가기
KERD
논문KCI 등재

영지버섯 균사체 발효 꾸지뽕 잔가지 추출물의 3T3-L1 지방전구세포 분화 억제 및 항염증 효과

Effect of extract from Maclura tricuspidata twig fermented with Ganoderma lucidum mycelium on adipocyte differentiation and inflammation in 3T3-L1 cells

박세은|김승|김기만
한국식품저장유통학회 (The Korean Society of Food Preservation)|2023.06|Food Science and Preservation|제30권 제3호|pp. 502-513

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

fermentationinflammationdifferentiation3T3-L1Maclura tricuspidata twig
주제분류식품과학

초록

This study aimed to evaluate the anti-adipogenic and anti-inflammation effects of extract from Maclura tricuspidata twig fermented with Ganoderma lucidum mycelium (EMFG) in 3T3-L1 preadipocytes. 3T3-L1 adipocytes were treated with 100, 200, 300 μg/mL of EMFG. The result showed that EMFG dose-dependently inhibited the accumulation of intracellular lipid content in differentiated 3T3-L1 adipocytes and enhanced increase of adiponectin release and inhibition of leptin release. EMFG treatment reduced expression of adipogenic transcriptional factor such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα). EMFG also decreased production of lipopolysaccharide (LPS)-induced inflammatory cytokine [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1)] and the protein expression of cyclooxygenase-2 (COX-2) and inducible NOS (iNOS) in differentiated 3T3-L1 adipocytes. The study demonstrated that EMFG inhibited adipogenesis and inflammation in a dose-dependent manner. These findings suggest that EMFG may have potential as an anti-obesity and anti- metabolic disease agent that works by inhibiting adipogenesis and inflammation.

Abstract

This study aimed to evaluate the anti-adipogenic and anti-inflammation effects of extract from Maclura tricuspidata twig fermented with Ganoderma lucidum mycelium (EMFG) in 3T3-L1 preadipocytes. 3T3-L1 adipocytes were treated with 100, 200, 300 μg/mL of EMFG. The result showed that EMFG dose-dependently inhibited the accumulation of intracellular lipid content in differentiated 3T3-L1 adipocytes and enhanced increase of adiponectin release and inhibition of leptin release. EMFG treatment reduced expression of adipogenic transcriptional factor such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα). EMFG also decreased production of lipopolysaccharide (LPS)-induced inflammatory cytokine [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1)] and the protein expression of cyclooxygenase-2 (COX-2) and inducible NOS (iNOS) in differentiated 3T3-L1 adipocytes. The study demonstrated that EMFG inhibited adipogenesis and inflammation in a dose-dependent manner. These findings suggest that EMFG may have potential as an anti-obesity and anti- metabolic disease agent that works by inhibiting adipogenesis and inflammation.

참고문헌 (40)

  1. Ali AT, "Adipocyte and adipogenesis", Eur J Cell Biol, 92, pp.229~236 (2013)
  2. Anusree SS, "Insulin resistance in 3T3-L1 adipocytes by TNF-α is improved by punicic acid through upregulation of insulin signalling pathway and endocrine function, and downregulation of proinflammatory cytokines", Biochimie, 146, pp.79~86 (2018)
  3. Bastard JP, "Recent advances in the relationship between obesity, inflammation, and insulin resistance", Eur Cytokine Netw, 17, pp.4~12 (2006)
  4. Chen AW, "Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes", PLoS One, 14, pp.e0217090~ (2019)
  5. Hyehyun Hong, "Anti-inflammatory activity of 6-O-phospho-7-hydroxycoumarin in LPS-induced RAW 264.7 cells", Journal of Applied Biological Chemistry, 65(1), pp.33~41 (2022), 한국응용생명화학회[DOI]
  6. 최학주, "꾸지뽕나무 추출물의 피부 생리 활성", 한국응용과학기술학회지, 32(2), pp.260~274 (2015), 한국응용과학기술학회
  7. Dilworth L, "Diabetes mellitus and its metabolic complications : The role of adipose tissues", Int J Mol Sci, 22, pp.7644~ (2021)
  8. Ekmen N, "Leptin as an important link between obesity and cardiovascular risk factors in men with acute myocardial infarction", Indian Heart J, 6, pp.132~137 (2016)
  9. Engin AB, "Adipocyte-macrophage cross-talk in obesity", Adv Exp Med Biol, 960, pp.327~343 (2017)
  10. Guzik TJ, "The role of infiltrating immune cells in dysfunctional adipose tissue", Cardiovasc Res, 113, pp.1009~1023 (2017)
  11. 한효선, "꾸지뽕나무 가지 추출물을 이용한 미백 기능성 화장품 소재 개발", 아시안뷰티화장품학술지, 14(3), pp.317~328 (2016), 한국피부과학연구원[DOI]
  12. Haslam DW, "Obesity", Lancet, 366, pp.1197~1209 (2005)
  13. 홍준영, "상황버섯 균사체로 고체 발효한 수삼의 지질 저하 효과", 석사, 식품영양학과, pp.2014
  14. Hsieh CC, "Lunasin attenuates obesity-related inflammation in RAW264.7 cells and 3T3-L1 adipocytes by inhibiting inflammatory cytokine production", PLoS One, 12, pp.e0171969~ (2017)
  15. Hu ED, "Transdifferentiation of myoblasts by the adipogenic transcription factors PPARγ and C", EBPα, 1995, pp.92, 9856~9860
  16. 정은미, "배양기간에 따른 버섯균사체 인삼배양물의 항산화활성", 한국식품영양과학회지, 39(9), pp.1346~1352 (2010), 한국식품영양과학회
  17. Kallen CB, "Antidiabetic thiazolidinediones inhibit leptin(ob)gene expression in 3L3-L1adipocytes", Proc Natl Acad Sci USA, 93, pp.5793~5796 (1996)
  18. Kern PA, "Adipose tissue tum or necrosis factor and interleukin-6 expression in hum an obesity and insulin resistance", Am J Phy siol Endocrinol Metab, 280, pp.745~751 (2001)
  19. 김단비, "버섯균사체를 달리한 발효가시오가피 추출물의 항산화 활성 및 아질산염 소거능", 한국식품과학회지, 46(2), pp.205~212 (2014), 한국식품과학회[DOI]
  20. 김훈, "Enhancement of Immunostimulation and Anti-metastasis in Submerged Culture of Bearded Tooth Mushroom (Hericium erinaceum) Mycelia by Addition of Ginseng Extract", Food Science and Biotechnology, 19(5), pp.1259~1266 (2010), 한국식품과학회
  21. Kim HJ, "Hepatoprotective effect of Cudrania tricuspidata trunk hot-water extract against CCl4-induced hepatic inflammation rat", Ph D, pp.2017
  22. Hye Lin Kim, "Effect of saccharin on inflammation in 3T3-L1 adipocytes and the related mechanism", Nutrition Research and Practice, 14(2), pp.109~116 (2020), 한국영양학회[DOI]
  23. 김미현, "3종의 버섯균사체로 발효한 천년초 에탄올 추출물의 생리활성", 한국식품영양학회지, 28(4), pp.620~627 (2015), 한국식품영양학회
  24. 김옥경, "산화적 손상에 대한 꾸지뽕나무 잎, 열매 및 줄기 추출물의 간세포 보호효과", 한국식품영양과학회지, 41(1), pp.7~13 (2012), 한국식품영양과학회
  25. Kim SH, "Antiinflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus", J Ethnopharmacol, 93, pp.141~146 (2004)
  26. 고영우, "더덕, 도라지 표고균사발효물의 유용성분 및 생리활성", 한국버섯학회지, 19(4), pp.300~309 (2021), 한국버섯학회[DOI]
  27. 권기석, "홍맥 추출물의 3T3-L1세포에 대한 지방 축적 저해 활성평가", 생명과학회지, 31(2), pp.192~198 (2021), 한국생명과학회[DOI]
  28. Landecho MF, "Relevance of leptin and other adipokines in obesity-associated cardiovascular risk", Nutrients, 11, pp.2664~ (2019)
  29. 이효정, "꾸지뽕 가지 추출물의 지방세포 분화 및 지방축적 억제 효과", Food Science and Preservation, 29(5), pp.825~835 (2022), 한국식품저장유통학회
  30. Lee IK, "Tumor cell growth inhibition and antioxydative activity of flavonoids from the stem bark of Cudrania tricuspidata", J Korean Soc Appl Bi, 37, pp.105~109 (1994)
  31. 임용래, "3T3-L1 지방전구세포에서 홍국균 균사체-고체발효 원두커피 추출물의 지방축적 억제효과", 한국식품영양과학회지, 43(4), pp.624~629 (2014), 한국식품영양과학회
  32. 박미영, "발아현미가 LPS로 유도된 지방세포의 염증반응에 미치는 영향", 한국식생활문화학회지, 33(4), pp.337~344 (2018), 한국식생활문화학회[DOI]
  33. 박미영, "Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes", Nutrition Research and Practice, 8(5), pp.516~520 (2014), 한국영양학회
  34. Rosen E, "Transcriptional targets in adipocyte biology", Expert Opin Ther Targets, 13, pp.975~986 (2009)
  35. Rosen ED, "Molecular regulation of adipogenesis", Annu Rev Cell Dev Bi, 16, pp.145~171 (2000)
  36. Rosenbaum M, "The role of leptin in human physiology", N Engl J Med, 341, pp.913~915 (1999)
  37. 서은영, "생강 성분인 (6)-Gingerol이 3T3-L1에서 지방세포 증식과 분화 과정에 미치는 영향", Journal of Nutrition and Health, 48(4), pp.327~334 (2015), 한국영양학회
  38. Shon MY, "Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms", Food Indus Nutr, 12, pp.51~57 (2007)
  39. Unamuno X, "Adipokine dysregulation and adipose tissue inflammation in human obesity", Eur J Clin Invest, 48, pp.e12997~ (2018)
  40. Wang XL, "Osthole inhibits inflammatory cytokine release through PPARα", γ-mediated mechanisms in LPSstimulated 3T3-L1 adipocytes, 2015, pp.37, 185~192

관련 논문AI추천

꾸지뽕 가지 추출물의 지방세포 분화 및 지방축적 억제 효과김승, 박세은, 이효정한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제5호지방세포 분화중인 3T3-L1 세포에서 영지추출물에 의한 지방관련 유전자와 전사인자의 발현 조절강경화, 윤현민, 이채우대한약침학회 · Journal of Pharmacopuncture2009 · 제12권 제2호3T3-L1 지방전구세포의 분화에 미치는 영지약침의 영향강경화, 윤현민, 이채우대한약침학회 · Journal of Pharmacopuncture2008 · 제11권 제3호꾸지뽕 열매 발효식초의 3T3-L1 세포에서 항염증 및 인슐린 저항성 개선 효과김승, 박세은한국식품저장유통학회 · Food Science and Preservation2021 · 제28권 제7호꾸지뽕 열매 발효식초의 LPS에 의해 유도된 3T3-L1 세포 염증반응 억제 효과김승, 박세은, 여수환한국식품저장유통학회 · Food Science and Preservation2020 · 제27권 제6호Anti-adipogenic effect of mulberry leaf ethanol extract in 3T3-L1 adipocytes박나영, 양수진, 임윤숙한국영양학회 · Nutrition Research and Practice2014 · 제8권 제6호

이 간행물의 다른 논문 (16건)

Erratum : Non-destructive quality prediction of domestic, commercial red pepper powder using hyperspectral imaging최지영, 조정석, 임정호, 김상섭pp. 224-234Erratum : 효소 및 미생물 복합체를 사용한 인비트로 루왁 커피의 품질 특성강혜미, 오신영, 강혜민, 권중호, 정용진pp. 287-299Quality characteristics of fresh cut apples during storage according to processing steps and browning inhibitor treatment장민선, 김건희, 조순덕, 정현나pp. 369-382Development and validation of ultra-fast quantitative real-time PCR method to differentiate between Oncorhynchus keta and Oncorhynchus mykiss양지영, 이한철, 김중범, 박민지pp. 383-394Chemical profiles and biological activities of essential oil of Citrus hystrix DC. peelsVuong Bao Thy, Do Minh Long, Tran Thi Phuong Nhung, Nguyen Le Quynh Nhu, Le Pham Tan Quocpp. 395-404
원문 보기