Bacterial Apoptosis-Like Death through Accumulation of Reactive Oxygen Species by Quercetin in Escherichia coli
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The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin.
Abstract
The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin.
참고문헌 (25)
- Rohatgi A, "Natural and synthetic plant compounds as anti-biofilm agents against Escherichia coli O157 : H7 biofilm", Infect. Genet. Evol, 95, pp.105055~ (2021)
- Paitan Y, "Current trends in antimicrobial resistance of Escherichia coli", Escherichia coli, a versatile pathogen, 2018, pp.181~211 (2018)
- Lim JY, "A brief overview of Escherichia coli O157 : H7 and its plasmid O157", J. Microbiol. Biotechnol, 20, pp.5~14 (2010)
- Hwang IS, "Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved", J. Med. Microbiol, 61, pp.1719~1726 (2012)
- Kim AR, "Phenolic compounds with IL-6 inhibitory activity from Aster yomena", Arch. Pharm. Res, 37, pp.845~851 (2014)
- Kumar S, "Chemistry and biological activities of flavonoids : an overview", ScientificWorldJournal, 2013, pp.162750~ (2013)
- Hwang IS, "Indole-3-carbinol generates reactive oxygen species and induces apoptosis", Biol. Pharm. Bull, 34, pp.1602~1608 (2011)
- Lucia Forte, "Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite : an in vitro osteoblast–osteoclast–endothelial cell co-culture study", Acta Biomater, 32, pp.298~308 (2016)
- Memariani H, "An overview on anti-biofilm properties of quercetin against bacterial pathogens", World J. Microbiol. Biotechnol, 35, pp.143~ (2019)
- Cushnie TP, "Antimicrobial activity of flavonoids", Int. J. Antimicrob. Agents, 26, pp.343~356 (2005)
- Reczek CR, "ROS-dependent signal transduction", Curr. Opin. Cell Biol, 33, pp.8~13 (2015)
- N Guaragnella, "Hydrogen peroxide and superoxide anion production during acetic acid-induced yeast programmed cell death", Folia Microbiol(Praha), 52, pp.237~240 (2007)
- Beckman JS, "Apparent hydroxyl radical production by peroxynitrite : implications for endothelial injury from nitric oxide and superoxide", Proc. Natl. Acad. Sci. USA, 87, pp.1620~1624 (1990)
- Mittler R, "ROS are good", Trends Plant Sci, 22, pp.11~19 (2017)
- Gabriela Diaz de Barboza, "Oxidative stress, antioxidants and intestinal calcium absorption", World J. Gastroenterol, 23, pp.2841~2853 (2017)
- Lee H, "Apigenin induces cell shrinkage in Candida albicans by membrane perturbation", FEMS Yeast Res, 18, pp.foy003~ (2018)
- Lee J, "Melittin triggers apoptosis in Candida albicans through the reactive oxygen species-mediated mitochondria", caspase-dependent pathway, 2014, pp.355, 36~42
- Yun J, "Cecropin A-induced apoptosis is regulated by ion balance and glutathione antioxidant system in Candida albicans", IUBMB Life, 68, pp.652~662 (2016)
- Yun DG, "Assessment of silibinin as a potential antifungal agent and investigation of its mechanism of action", IUBMB Life, 69, pp.631~637 (2017)
- Choi H, "Coprisin exerts antibacterial effects by inducing apoptosis-like death in E scherichia coli", IUBMB Life, 68, pp.72~78 (2016)
- María Marcén, "Oxidative stress in E. coli cells upon exposure to heat treatments", Int. J. Food Microbiol, 241, pp.198~205 (2017)
- Manfredini V, "Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage", Braz. J. Med. Biol. Res, 37, pp.159~165 (2004)
- Erental A, "Two programmed cell death systems in Escherichia coli : an apoptotic-like death is inhibited by the mazEF-mediated death pathway", PLoS Biol, 10, pp.e1001281~ (2012)
- Lee W, "Effect of apigenin isolated from Aster yomena against Candida albicans : apigenin-triggered apoptotic pathway regulated by mitochondrial calcium signaling", J. Ethnopharmacol, 231, pp.19~28 (2019)
- Lee J, "Cell selectivity-membrane phospholipids relationship of the antimicrobial effects shown by pleurocidin enantiomeric peptides", J. Pept. Sci, 15, pp.601~606 (2009)