Lycopene-induced hydroxyl radical causes oxidative DNA damage in Escherichia coli
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Lycopene, which is a well-known red carotenoid pigment, has been drawing scientific interest because of its potential biological functions. The current study reports that lycopene acts as a bactericidal agent by inducing reactive oxygen species (ROS)-mediated DNA damage in Escherichia coli. Lycopene treatment elevated the level of ROS—in particular, hydroxyl radicals (•OH) —which can damage DNA in E. coli. Lycopene-induced DNA damage in bacteria was confirmed and we also observed cell filamentation caused by cell division arrest, an indirect marker of the DNA damage repair system, in lycopene-treated E. coli. Increased RecA expression was observed, indicating activation of the DNA repair system (SOS response). To summarize, lycopene exerts its antibacterial effects by inducing •OH -mediated DNA damage that cannot be ameliorated by the SOS response. Lycopene may be a clinically useful adjuvant for current antimicrobial therapies.
Abstract
Lycopene, which is a well-known red carotenoid pigment, has been drawing scientific interest because of its potential biological functions. The current study reports that lycopene acts as a bactericidal agent by inducing reactive oxygen species (ROS)-mediated DNA damage in Escherichia coli. Lycopene treatment elevated the level of ROS—in particular, hydroxyl radicals (•OH) —which can damage DNA in E. coli. Lycopene-induced DNA damage in bacteria was confirmed and we also observed cell filamentation caused by cell division arrest, an indirect marker of the DNA damage repair system, in lycopene-treated E. coli. Increased RecA expression was observed, indicating activation of the DNA repair system (SOS response). To summarize, lycopene exerts its antibacterial effects by inducing •OH -mediated DNA damage that cannot be ameliorated by the SOS response. Lycopene may be a clinically useful adjuvant for current antimicrobial therapies.
참고문헌 (29)
- Arias CA, "Antibiotic-resistant bugs in the 21st century - a clinical super-challenge", N. Engl. J. Med, 360, pp.439~443 (2009)
- Basu A, "Tomatoes versus lycopene in oxidative stress and carcinogenesis: conclusions from clinical trials", Eur. J. Clin. Nutr, 61, pp.295~303 (2007)
- Brynildsen MP, "Potentiating antibacterial activity by predictably enhancing endogenous microbial ROS production", Nat. Biotechnol, 31, pp.160~165 (2013)
- Chandra RV, "Efficacy of lycopene in the treatment of gingivitis:a randomized, placebo-controlled clinical trial", J. Nutr, 138, pp.49~53 (2008)
- Chen J, "Early detection of DNA strand breaks in the brain after transient focal ischemia: implications for the role of DNA damage in apoptosis and neuronal cell death", J. Neurochem, 69, pp.232~245 (1997)
- Costa V, "Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights in to aging, apoptosis and disease", Mol. Aspects Med, 22, pp.217~246 (2001)
- Cozzi R, "Ascorbic acid and beta-carotene as modulators of oxidative damage", Carcinogenesis, 18, pp.223~228 (1997)
- Cui J, "Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain", FASEB J, 14, pp.955~967 (2000)
- Daniel B, "Quantification of sPSA-2ind uced early a nd late apoptosis c hanges i n neuronal c ell cultures using combined TUNEL and DAPI staining", Brain Res. Protoc, 13, pp.144~150 (2004)
- Ferullo DJ, "The stringent response and cell cycle arrest in Escherichia coli", PLoS Genet, 4, pp.1000300~ (2008)
- Foti JJ, "Oxidation of the guanine nucleotide pool underlies cell death by bactericidal antibiotics", Science, 334, pp.315~319 (2012)
- Furuya EY, "Antimicrobial-resistant bacteria in the community setting", Nat. Rev. Microbiol, 4, pp.36~45 (2006)
- Han CH, "Synergistic effect between lycopene and ciprofloxacin on a chronic bacterial prostatitis rat model", Int. J. Antimicrob. Agents, 31, pp.102~107 (2008)
- Huang D, "In situ detection of AP sites and DNA strand breaks with 3’-phosphate ends in ischemic mouse brain", FASEB J, 14, pp.407~417 (2000)
- Kaper JB, "Pathogenic Escherichia coli", Nat. Rev. Microbiol, 2, pp.123~140 (2004)
- Kaufmann WK, "DNA damage and cell cycle checkpoints", FASEB J, 10, pp.238~347 (1996)
- Klepser ME, "Influence of test conditions on antifungal time-kill curve results: proposal for standardized methods", Antimicrob. Agents Chemother, 42, pp.1207~1212 (1998)
- Kohanski MA, "A common mechanism of cellular death induced by bactericidal antibiotics", Cell, 130, pp.797~810 (2007)
- Lowe GM, "Lycopene and beta-carotene protect against oxidative damage in HT29cells at low concentrations but rapidly lose this capacity at higher doses", Free Radic. Res, 30, pp.141~151 (1999)
- Madeo F, "A yeast mutant showing diagnostic markers of early and late apoptosis", J. Cell Biol, 139, pp.729~734 (1997)
- Miller C, "SOS response induction by beta-lactams and bacterial defense against antibiotic lethality", Science, 305, pp.1629~1631 (2004)
- Phillips AJ, "Apoptosis induced by environmental stresses and amphothericin B in Candida albicans", Proc. Natl. Acad. Sci. USA, 100, pp.14327~14332 (2003)
- Sanchez L., "TCA protein precipitation" (2001), Protocols on line
- Sharma V, "Knockdown of recA gene expression by artificial small RNAs in Escherichia coli. Biochem", Biophys. Res. Commun, 430, pp.256~259 (2012)
- Shkilnyj P, "Bacteriophage 434Hex protein prevents RecA-mediated repressor autocleavage", Viruses, 5, pp.111~126 (2013)
- SUNG, WOO SANG, "Damage to the Cytoplasmic Membrane and Cell Death Caused by Lycopene in Candida albicans", Journal of Microbiology and Biotechnology, 17(11), pp.1797~1804 (2007), 한국미생물·생명공학회
- Tzur A, "Cell growth and size homeostasis in proliferating animal cells", Science, 325, pp.167~171 (2009)
- Zelezetsky I, "Controlled alteration of the shape and conformational stability of alpha-helical cell-lytic peptides:effect on mode of action and cell specificity", Biochem. J, 390, pp.177~188 (2005)
- Zunino SJ, "Parthenolide induces significant apoptosis and production of reactive oxygen species in high-risk pre-B leukemia cells", Cancer Lett, 254, pp.119~127 (2007)