Purification and Characterization of a β-Glucosidase from Aspergillus niger and Its Application in the Hydrolysis of Geniposide to Genipin
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.
Abstract
An extracellular β-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 β-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at 65°C and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The β-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 β-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.
참고문헌 (21)
- Almog J, "Genipin - a novel fingerprint reagent with colorimetric and fluorogenic activity", J. Forensic Sci, 49, pp.255~257 (2004)
- Bowers EM, "Salt effects on β-glucosidase: pH-profile narrowing", Biochim. Biophys. Acta, 1774, pp.1500~1507 (2007)
- Bradford MM., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Chen M, "Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementation", Enzyme Microb. Technol, 46, pp.444~449 (2010)
- Chen SC, "A novel pH-sensitive hydrogel composed of N,O-carboxymethyl chitosan and alginate cross-linked by genipin for protein drug delivery", J. Control. Release, 96, pp.285~300 (2004)
- Chiono V, "Genipin-crosslinked chitosan", gelatin blends for biomedical applications, 2008, pp.19, 889~898
- Diao JS, "Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N+ implantation", J. Radiat. Res. Radiat. Process, 28, pp.345~351 (2010)
- Djerassi C, "Naturally occurring oxygen heterocyclics. IX. Isolation and characterization of genipin", J. Org. Chem, 25, pp.2174~2177 (1960)
- Djerassi C, "Terpenoids. XLVII. The structure of genipin", J. Org .Chem, 26, pp.1192~1206 (1961)
- Henrissat B., "A classification of glycosyl hydrolases based on amino acid sequence similarities", Biochem. J, 280, pp.309~316 (1991)
- Henrissat B, "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities", Biochem. J, 293, pp.781~788 (1993)
- Koo HJ, "Antiinflammatory effects of genipin, an active principle of gardenia", Eur. J. Pharmacol, 495, pp.201~208 (2004)
- Lee SW, "Colorimetric determination of amino acids using genipin from Gardenia jasminoides", Anal. Chim. Acta, 480, pp.267~274 (2003)
- Lineweaver H, "The determination of enzyme dissociation constants", J. Am. Chem. Soc, 56, pp.658~666 (1934)
- Pei JJ, "Thermoanaerobacterium thermosaccharolyticum β-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose", Biotechnol. Biofuels, 5, pp.31~40 (2012)
- Saibi W, "Purification and biochemical characterization of an atypical β-glucosidase from Stachybotrys microspora", J. Mol. Catal. B Enzym, 72, pp.107~115 (2011)
- Wang JF, "Purification and characterization of geniposide-hydrolyzing β-glucosidase from Aspergillus niger", Mycosystema, 29, pp.683~690 (2010)
- Xu MM, "Microbial transformation of geniposide in Gardenia jasminoides Ellis into genipin by Penicillium nigricans", Enzyme Microb. Technol, 42, pp.440~444 (2008)
- Yang YS, "Transformation of geniposide into genipin by immobilized β-glucosidase in a two-phase aqueous-organic system", Molecules, 16, pp.4295~4304 (2011)
- Yuan Y, "The effect of cross-linking of chitosan microspheres with genipin on protein release", Carbohyd. Polym, 68, pp.561~567 (2007)
- Zhang CY, "Genipin inhibits UCP2-mediated proton leak and acutely reverses obesity- and high glucose-induced beta cell dysfunction in isolated pancreatic islets", Cell Metab, 3, pp.417~427 (2006)