Retinoic Acid가 돼지 지방세포의 분화에 미치는 영향
Effects of Retinoic Acid on Differentiation of Pig Preadipocytes
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The current study was undertaken to determine the effects of retinoic acid(RA) on differentiation of pig preadipocyes. Pig preadipocytes were isolated from the backfat of new-born pigs with collagenase digestion. The isolated cells were seeded on MEM/F12 media and washed one day after seeding(day 0). Ten μM RA treated on day 0~2, day 2~4 and day 4~6 decreased differentiation of pig preadipocytes(P<0.01) with the highest inhibition found on day 0~2 treatment. One, 5 and 10μM of RA showed inhibitory action on differentiation in dose-dependent manner. The inhibitory effects of RA on proliferation of pig preadipocytes were smaller, compared to the effects on differentiation.
Abstract
The current study was undertaken to determine the effects of retinoic acid(RA) on differentiation of pig preadipocyes. Pig preadipocytes were isolated from the backfat of new-born pigs with collagenase digestion. The isolated cells were seeded on MEM/F12 media and washed one day after seeding(day 0). Ten μM RA treated on day 0~2, day 2~4 and day 4~6 decreased differentiation of pig preadipocytes(P<0.01) with the highest inhibition found on day 0~2 treatment. One, 5 and 10μM of RA showed inhibitory action on differentiation in dose-dependent manner. The inhibitory effects of RA on proliferation of pig preadipocytes were smaller, compared to the effects on differentiation.
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