본문 바로가기
KERD
논문KCI 등재

Efficacy of Lactate-Utilizing Bacteria Isolated from the Rumen in Alleviating Subacute Ruminal Acidosis In Vitro

김경진|이상무|박진현|김창현|김은중|윤두학|배귀석|Tabita Dameria Marbun|장문백|문병흠
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2021|농업생명과학연구 (Journal of Agriculture & Life Science)|제55권 제3호|pp. 73-80|DOI https://doi.org/10.14397/jals.2021.55.3.73

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

in vitroRumen fermentationRumen lactate-utilizing bacteriaSubacute ruminal acidosis

초록

This study was conducted to evaluate the effect of rumen origin lactate-utilizing bacteria (LUB) as one of the potential treatments on subacute ruminal acidosis (SARA) during in vitro challenge compared to buffering agents (NaHCO3, sea minerals, MgO) and direct-fed microorganism (yeast). We hypothesized that rumen LUB (RLUB) could be a potential treatment to treat ruminal acidosis. The supplementation level of other treatments was determined by referring to previous studies in the literature. The 108 CFU/g freeze-dried RLUB isolated from Hanwoo cattle were compared with 0.1% NaHCO3, 0.8% of MgO, 0.5% sea mineral and 0.4% yeast during in vitro SARA challenge. Rumen fluid collected from one cannulated Holstein and one Hanwoo steer fed by maize silage was mixed with 0.5g feed consisted of 0.05g forage and 0.45g concentrate. These mixtures were incubated in triplication for 3, 6, 12 and 24h. After 6h of incubation, along with MgO and sea minerals, RLUB treatment showed higher (p<0.05) ㏗ values than control with no significant differences in total volatile fatty acid concentration. However, in the same period, the propionate concentration and A:P ratio were higher in RLUB than in the other treatment (p<0.05), which might alter the fermentation pattern. On the other hand, the RLUB treatment produced a higher (p<0.05) ammonia-N concentration. Based on these results, we can conclude that RLUB might have the potential to alleviate SARA. Nonetheless, further study on its mechanism in SARA is required, especially with live animals.

Abstract

This study was conducted to evaluate the effect of rumen origin lactate-utilizing bacteria (LUB) as one of the potential treatments on subacute ruminal acidosis (SARA) during in vitro challenge compared to buffering agents (NaHCO3, sea minerals, MgO) and direct-fed microorganism (yeast). We hypothesized that rumen LUB (RLUB) could be a potential treatment to treat ruminal acidosis. The supplementation level of other treatments was determined by referring to previous studies in the literature. The 108 CFU/g freeze-dried RLUB isolated from Hanwoo cattle were compared with 0.1% NaHCO3, 0.8% of MgO, 0.5% sea mineral and 0.4% yeast during in vitro SARA challenge. Rumen fluid collected from one cannulated Holstein and one Hanwoo steer fed by maize silage was mixed with 0.5g feed consisted of 0.05g forage and 0.45g concentrate. These mixtures were incubated in triplication for 3, 6, 12 and 24h. After 6h of incubation, along with MgO and sea minerals, RLUB treatment showed higher (p<0.05) ㏗ values than control with no significant differences in total volatile fatty acid concentration. However, in the same period, the propionate concentration and A:P ratio were higher in RLUB than in the other treatment (p<0.05), which might alter the fermentation pattern. On the other hand, the RLUB treatment produced a higher (p<0.05) ammonia-N concentration. Based on these results, we can conclude that RLUB might have the potential to alleviate SARA. Nonetheless, further study on its mechanism in SARA is required, especially with live animals.

참고문헌 (46)

  1. Aikman PC, "Rumen ㏗ and fermentation characteristics in dairy cows supplemented with Megasphaera elsdenii NCIMB 41125in early lactation", J. Dairy Sci, 94, pp.2840~2849 (2011)
  2. Andersen PH, "Effect of feeding regimen on concentration of free endotoxin in ruminal fluid of cattle", J. Anim. Sci, 72, pp.487~491 (1994)
  3. AOAC, "Official methods of analysis" (2005), AOAC International
  4. Arik HD, "Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in cattle", J. Anim. Physiol. Anim. Nutr, 103, pp.416~426 (2019)
  5. Barnes A, "In vitro rumen fermentation of soluble and non-soluble polymeric carbohydrates in relation to ruminal acidosis", Ann. Microbiol, 68, pp.1~8 (2018)
  6. Beauchemin K, "Effects of particle size of alfalfa-based dairy cow diets on chewing activity, ruminal fermentation and milk production", J. Dairy Sci, 86, pp.630~643 (2003)
  7. Beauchemin KA, "Effects of barley grain processing on the site and extent of digestion of beef feedlot finishing diets", J. Anim. Sci, 79, pp.1925~1936 (2001)
  8. Beuvink JMW, "Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro", Appl. Microbiol. Biotechnol, 37, pp.505~509 (1992)
  9. Block E, "Manipulation of dietary cation-anion difference on nutritionally related production diseases, productivity and metabolic responses of dairy cows", J. Dairy Sci, 77, pp.1437~1450 (1994)
  10. Burrin D, "Response to monensin in cattle during subacute acidosis", J. Anim. Sci, 63, pp.888~893 (1986)
  11. Calsamiglia S, "Is subacute ruminal acidosis a ㏗ related problem? Causes and tools for its control", Anim. Feed Sci. Technol, 172, pp.42~50 (2012)
  12. Chaney AL, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
  13. Chen L, "Megasphaera elsdenii lactate degradation pattern shifts in rumen acidosis models", Front. Microbiol, 10, pp.162~172 (2019)
  14. Cooper R, "Effects of imposed feed intake variation on acidosis and performance of finishing steers", J. Anim.Sci, 77, pp.1093~1099 (1999)
  15. Counotte GH, "Regulation of lactate metabolism in the rumen", Vet. Res. Commun, 5, pp.101~115 (1981)
  16. Cruywagen CW, "The effect of buffering dairy cow diets with limestone, calcareous marine algae or sodium bicarbonate on ruminal ㏗ profiles, production responses and rumen fermentation", J. Dairy Sci, 98, pp.5506~5514 (2015)
  17. Enemark JMD, "The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review", Vet. J, 176, pp.32~43 (2008)
  18. Erdman R, "Dietary sodium bicarbonate and magnesium oxide for early postpartum lactating dairy cows: Effects of production, acid-based metabolism and digestion", J. Dairy Sci, 65, pp.712~731 (1982)
  19. Erwin ES, "Volatile fatty acid analyses of blood and rumen fluid by gas chromatography", J. Dairy Sci, 44, pp.1768~1771 (1961)
  20. Goad D, "Ruminal microbial and fermentative changes associated with experimentally induced subacute acidosis in steers", J. Anim. Sci, 76, pp.234~241 (1998)
  21. Goto H, "Effects of a bacterial probiotic on ruminal ㏗and volatile fatty acids during subacute ruminal acidosis (SARA) in cattle", J. Vet. Sci, 78(10), pp.1595~1600 (2016)
  22. Gozho GN, "Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced subacute ruminal acidosis in dairy cows", J. Dairy Sci, 90, pp.856~866 (2007)
  23. Gozho GN, "Subacute ruminal acidosis induces ruminal lipopolysaccharide endotoxin release and triggers an inflammatory response", J. Dairy Sci, 88, pp.1399~1403 (2005)
  24. Henning PH, "The potential of Megasphaera elsdenii isolates to control ruminal acidosis", Anim. Feed Sci. Technol, 157, pp.13~19 (2010)
  25. Kleen JL, "Subacute ruminal acidosis (SARA): A review", J. Vet. Med. A. Physiol. Pathol. Clin. Med, 50, pp.406~414 (2003)
  26. Klieve AV, "Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high grain diets", J. Appl. Microbiol, 95, pp.621~630 (2003)
  27. Krause KM, "Inducing subacute ruminal acidosis in lactating dairy cows", J. Dairy Sci, 88, pp.3633~3639 (2005)
  28. Lee MH, "Isolation of anaerobic bacteria used for feed additives which are able to alleviate ruminal acidosis", Master's, Department of Animal Life and Environmental Science, pp.2018
  29. Mackie R, "Microbiological and chemical changes in the rumen during the stepwise adaptation of sheep to high concentrate diets", J. Agric. Sci, 90, pp.241~254 (1978)
  30. Mackie RI, "Changes in lactate-producing and lactate-utilizing bacteria in relation to ㏗ in the rumen of sheep during stepwise adaptation to a high-concentrate diet", Appl. Environ. Microbiol, 38, pp.422~430 (1979)
  31. Mazon G, "Effects of a Megasphaera elsdenii oral drench on reticulorumen ㏗ dynamics in lactating dairy cows under subacute ruminal acidosis challenge", Anim. Feed Sci. Technol, 261, pp.114404~ (2020)
  32. Menke KH, "The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro", J. Agric. Sci, 93, pp.217~222 (1979)
  33. Nagaraja TG, "Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook", J. Dairy Sci, 90, pp.E17~E38 (2007)
  34. Nocek JE, "Ruminal supplementation of direct-fed microbials on diurnal ㏗variation and in situ digestion in dairy cattle", J. Dairy Sci, 85, pp.429~433 (2002)
  35. Nocek JE, "Bovine acidosis: Implications on laminitis", J. Dairy Sci, 80, pp.1005~1028 (1997)
  36. Pilgrim AF, "Synthesis of microbial protein from ammonia in the sheep's rumen and the proportion of dietary nitrogen converted into microbial nitrogen", Br. J. Nutr, 24, pp.589~598 (1970)
  37. Plaizier JC, "Subacute ruminal acidosis (SARA), endotoxins and health consequences", Anim. Feed Sci. Technol, 172, pp.9~21 (2012)
  38. Plaizier JC, "Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences", Vet. J, 176, pp.21~31 (2008)
  39. Rodríguez F, "Control of lactate accumulation in ruminants using Prevotella bryantii", Doctoral, Veterinary Microbiology and Preventive Medicine, pp.2003
  40. Santra A, "Effect of dietary sodium bicarbonate supplementation on fermentation characteristics and ciliate protozoal population in rumen of lambs", Small Rumin. Res, 47, pp.203~212 (2003)
  41. 서자겸, "Direct-fed Microbials for Ruminant Animals", Animal Bioscience, 23(12), pp.1657~1667 (2010), 아세아·태평양축산학회
  42. Shaver RD, "Dietary buffers for dairy cattle" (1988), University of WisconsinExtension
  43. Tilley JMA, "A two‐stage technique for the in vitro digestion of forage crops", Grass Forage Sci, 18, pp.104~111 (1963)
  44. Umucalilar HD, "Alteration in ruminal fermentation: The effect of Megasphaera elsdenii inoculation on subacute ruminal acidosis (SARA) in vitro", Sci. Papers Ser. D. Anim. Sci, 57, pp.115~117 (2014)
  45. Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3583~3597 (1991)
  46. Yoon I, "Effects of Saccharomyces cerevisiae and Aspergillus oryzae cultures on ruminal fermentation in dairy cows", J. Dairy Sci, 79, pp.411~417 (1996)

관련 논문AI추천

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattleDong Xianwen, Guan Jiuqiang, Hu Rui, Kang Kun, Luo Xiaolin, Peng Quanhui, Shah Ali Mujtaba, Wanapat Metha, Wang Zhisheng, Zhang Xiangfei아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제2호Effect of Lactobacillus mucosae on In vitro Rumen Fermentation Characteristics of Dried Brewers Grain, Methane Production and Bacterial Diversity김선호, 배귀석, 이상석, 장문백, 정창대, 최연재, Alvin P. Soriano, MAMUAD LOVELIA아세아·태평양축산학회 · Animal Bioscience2014 · 제27권 제11호Effect of active and heat-killed Clostridium butyricum on in vitro gas production, ruminal fermentation parameters, and microbiota at varying media pH levelsHu Guanghui, Jiao Peixin, Lin Xu, Liu Jincheng, Xie Xiaolai, Xin Hangshu, Zhang Xinlong, Zhang Zhiyue, Zhu Hongxu아세아·태평양축산학회 · Animal Bioscience2026 · 제39권 제1호Bifidobacterium ruminantium 첨가가 청보리와 티모시 조사료의 in vitro 반추위 내발효성상과 메탄 발생에 미치는 영향김세진, 김창현, 최정호동물생명과학연구소 · 동물자원연구2016 · 제27권 제1호Effects of nitrate in combination with lactate on rumen fermentation and methane production in an in vitro batch cultureBae Beomseok, Lee Chanhee, Park Kiyeon, Pope Logan아세아·태평양축산학회 · Animal Bioscience2025 · 제38권 제8호Mitigating Methane Emissions and Enhancing Rumen Microbial Diversity with Native Rumen Microbes Supplementation김선호, 손아랑, 이상석, 이성대, Michelle Miguel, Mozart Nuzul Aprilliza AM농업생명과학연구원 · 농업생명과학연구2025 · 제59권 제3호

이 간행물의 다른 논문 (14건)

왕피천유역 생태․경관보전지역의 관속식물상 - 수하계곡(경북 영양)을 중심으로 -정문화, 유주한pp. 1-16오아시스 전통의학정보포털 및 고의서 빅데이터를 활용한 한약재 과체(瓜蒂)의 고문헌 분석 및 활용 가치 제고박정환, 김한솔, 이지혜, 강영민, 전종욱, 김대욱, 임남희, 정유성pp. 17-31Sensitivity Variation of Fusarium oxysporum f. sp. fragariae, Strawberry Wilt Pathogen against Biocontrol Agent김다란, 채대한, 곽연식pp. 33-42산지복구비 산출체계에 관한 이해관계자 집단 간의 의식성향 비교서정일, 이상인, 권형근, 구기운pp. 43-53경남 연화산 소나무림의 지위지수 추정윤준혁, 전형국, 배은지, 손영모, 이정환pp. 55-59
원문 보기