흰쥐의 좌골신경 압좌손상 후 저강도 레이저 치료가 이질통에 미치는 영향
Effects of Low Level Laser (He-Ne) Therapy on the Allodynia after Sciatic Compressed Nerve Injury in Rats
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본 연구는 좌골신경 압좌손상 시 He-Ne 저강도 레이저 치료를 실시하여 근력 약화보다 훨씬 많이 동반되는 감각기능에서의 통증완화 효과를 알아보는데 목적을 두었다. 실험 대상은 체중 200~300mg의 건강한 Spraque-Dawley계 웅성 흰쥐를 동일한 조건에서 사육한 6주령으로 치료군 8마리와 통제군 7마리를 사용하였다. 통제군과 실험군의 좌골신경 압좌 손상을 위하여 왼쪽 대퇴 외측부의 피부 1.5cm 절개하고 주위조직의 손상을 최소화하며 좌골신경을 노출시킨 후, 지혈겸자(hemostrtic forceps)를 이용하여 압좌손상을 유발하였다. 레이저 치료는 좌골신경압좌손상이 유발된 후 3일째부터 시작하여 2주 동안 매일 측정하였다. 4mJ/㎠(1일 1회 4분 10초) 저강도의 레이저를 좌골신경압좌 손상된 부위에 일정하게 조사하였다. 좌골신경압좌 손상의 정도와 저강도 레이저 치료의 효과를 확인하기 위한 감각기능 평가는 수술 전 1일, 수술 후 1, 4, 7, 14, 그리고 21일 레이저 치료 30분 후에 기계적 이질통과 냉 이질통으로 흰쥐의 좌우 뒷발바닥에서 평가하였다. 본 실험의 자료분석은 t-test를 사용하여 각 실험군 간의 통계학적 유의성을 검증하였으며, p의 값이 .05미만일 경우 유의한 수준으로 판정하였다. 본 실험의 결과는 실험군과 통제군 모두 기계적 이질통의 감소를 보였으나 실험군에서 더 큰 감소의 효과를 볼 수 있었다(p<.05). 냉 이질통에 대한 측정에서는 실험실의 더운 온도 및 주변 환경의 원인으로 신뢰할 수 있는 결과를 얻을 수 없었다. 그리고 수술 직후 1일부터 치료후 12일까지의 발모양을 사진으로 찍어 비교해본바 비정상적인 자세에서 정상적인 자세로의 현저한 변화가 있음이 육안으로 확인되었다.
Abstract
Peripheral nerve injury may lead to sever functional loss including motor impairment and long-lasting neuropathic pain. Low level laser therapy(LLLT) has been used for the treatment of a variety of diseases. The therapeutic properties of LLLT irradiation has been focused on the acute pain with inflammation and wound healing. This study investigated the effect of LLLT on chronic neuropathic pain following sciatic nerve injury. Sciatic nerve injury was made left side sciatic nerve under enflurane anesthesia. A He-Ne laser of 0.4J/cm2 were used for irradiation. The allodynia was measured before and after laser treatment application. There was significant difference in the allodynia between two groups. Applied laser irradiation group alleviatied pain than non-applied group. There was on significant difference in the cold allodynia. In conclusion, our study demonstrates that LLLT with He-Ne lasers can promote the healing of operative wounds and alleviate the allodynia.
참고문헌 (36)
- 김명옥, "좌골신경 손상의 정도와 손상 후 운동량에 따른 신경회복에 관한 실험적 연구", 재활의학, pp.2002
- 김영철, "좌골신경 손상된 랫드에서 초음파 검사에 의한 좌골신경 기능지수 향상효과", Laboratory Animal Research, 20(2), pp.171~176 (2004), 한국실험동물학회
- 김현애, "초음파가 흰쥐의 좌골신경 압좌손상 후 척수내 Neural Cell Adhesion Molecules의 발현에 미치는 영향", 대한물리치료학회지, 19(2), pp.41~55 (2007), 대한물리치료학회
- 김권영, "손상된 흰쥐의 좌골신경에 저출력 레이저 조사후 전기생리학적 변화", 생명과학회지, 16(1), pp.114~119 (2006), 한국생명과학회
- Aimbire, F., "Effect of LLLT Ga-Al-As(685nm) on LPS-induced inflammation of the airway and lung in the rat", Lasers in Medical Science, 20, pp.11~20 (2005)
- Babapour, R., "Low- energy laser systems", Clinics in Dermatology, 13, pp.87~90 (1995)
- Basford, J. R., "Low intensity laser therapy: still not an established clinical tool", Lasers in Surgery and Medicine, 16, pp.331~342 (1995)
- Basford, J. R., "Does low-intensity helium-neon laser irradiation alter sensory nerve active potentials or distal latencies?", Lasers in Surgery and Medicine, 10, pp.35~39 (1990)
- Beckerman, H., "The efficacy of laser therapy for musculoskeletal and skin disorders: a criteria-based meta-analysis of randomized clinical trials", Physical Therapy, 72, pp.483~491 (1992)
- Bain, J. R., "Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat", Plastic and Reconstructive Surgery, 83, pp.129~136 (1989)
- Bennett, G. J., "A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man", Pain, 33, pp.87~107 (1988)
- De Bie, R. A., "Low-level laser therapy in ankle sprains: a randomized clinical trial", Archives of Physical Medicine and Rehabilitation, 79, pp.1415~1420 (1998)
- Devor, M., "What`s in a laser beam for pain therapy?", Pain, 43, pp.139~146 (1990)
- Enwemeka, C. S., "Laser biostimulation of healing wounds: specific effects and mechanisms of action", Journal of Orthopaedic and Sports Physical Therapy, 9, pp.333~338 (1988)
- Global information, "Peripheral neuropathy and Neuropathic pain" (2008), Global Information, Inc
- Jarvis, D., "Electrophysiologic recording and thermodynamic modeling demonstrate that helium-neon laser irradiation does not affect peripheral Adelta- or C-fiber nociceptors", Pain, 43, pp.235~242 (1990)
- Kim, J., "Effects of morphine on mechanical allodynia in a rat model of central neuropathic pain", Neuroreport, 23(14), pp.1017~1020 (2003)
- Kim, J., "Cold and mechanical allodynia in both hindpaws and tail following thoracic spinal cord hemisection in rats: time courses and their correlates", Neuroscience Letters, 343, pp.200~204 (2003)
- King, C. E., "Effect of helium-neon laser auriculotherapy on experimental pain threshold", Physical Therapy, 70, pp.24~30 (1990)
- Lee, S. K., "Peripheral nerve injury and repair", Journal of the American Academy of Orthopaedic Surgeons, 8, pp.243~252 (2000)
- Lowe, A. S., "Effect of low intensity monochromatic light therapy(890nm) on a radiation- impaired, wound-healing model in murine skin", Lasers in Surgery and Medicine, 23, pp.291~298 (1998)
- Maegawa, Y., "Effects of near-infrared low-level laser irradiation on microcirculation", Lasers in Ssurgery and Medicine, 27, pp.427~437 (2000)
- Marchettini, P., "Painful peripheral neuropathies", Current Neuropharmacology, 4, pp.175~181 (2006)
- Mester, E., "The use of the laser beam in therapy", Orvosi Hetilap, 107, pp.1012~1016 (1996)
- Miloro, M., "Low-level laser effect on neural regeneration in Gore-Tex tubes. Oral Surgery, Oral Medicine, Oral Pathology", Oral Radiology, and Endodontics, 93, pp.27~34 (2002)
- Okamoto, H., "Dye-mediated bactericidal effect of He-Ne laser irradiation on oral microorganisms", Lasers in Surgery and Medicine, 12, pp.450~458 (1992)
- Rochkind, S., "Effects of laser irradiation on the spinal cord for the regeneration of crushed peripheral nerve in rats", Lasers in Surgery and Medicine, 28, pp.216~219 (2001)
- Rochkind, S., "Respones of peripheral nerve to He-Ne laser: experimental studies", Lasers in Surgery and Medicine, 7, pp.441~443 (1987)
- Rochkind, S., "Electrophysiological effect of HeNe laser on normal and injured sciatic nerve in the rat", Acta Neurochirurgica, 83, pp.125~130 (1986)
- Rochkind, S., "Spinal cord response to laser treatment of injured peripheral nerve", Spine, 15, pp.6~10 (1990)
- Sarikcioglu, L., "Yasargil-Phynox aneurysm clip: a simple and reliable device for making a peripheral nerve injury", International Journal of Neuroscience, 113, pp.455~464 (2003)
- Shamir, M. H., "Double-blind randomized study evaluating regeneration of the rat transected sciatic nerve after suturing and postoperative low-power laser treatment", Journal of Reconstructive Microsurgery, 17, pp.133~137 (2001)
- Van Meeteren, N. L., "Exercise training improves functional recovery and motor nerve conduction velocity after sciatic nerve crush lesion in the rat", Archives of Physical Medicine and Rehabilitation, 78, pp.70~77 (1997)
- Walker, M. D., "Effect of low intensity laser irradiation (660nm) on a radiation-impaired wound-healing model in murine skin", Lasers in Surgery and Medicine, 26, pp.41~47 (2000)
- Wollman, Y., "In vitro cellular processes sprouting in cortex microexplants of adult rat brains induced by low power laser irradiation", Neurological Research, 20, pp.470~472 (1998)
- Zarkovic, N., "Effect of semiconductor GaAs laser irradiation on pain perception in mice", Lasers in Surgery and Medicine, 9, pp.63~66 (1989)