Comparative Analysis of Clinical Samples Showing Weak Serum Reaction on AutoVue System Causing ABO Blood Typing Discrepancies
1
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Background: ABO blood typing in pre-transfusion testing is a major component of the high workload in blood banks that therefore requires automation. We often experienced discrepant results from an automated system, especially weak serum reactions. We evaluated the discrepant results by the reference manual method to confirm ABO blood typing. Methods: In total, 13,113 blood samples were tested with the AutoVue system; all samples were run in parallel with the reference manual method according to the laboratory protocol. Results: The AutoVue system confirmed ABO blood typing of 12,816 samples (97.7%), and these results were concordant with those of the manual method. The remaining 297 samples (2.3%) showed discrepant results in the AutoVue system and were confirmed by the manual method. The discrepant results involved weak serum reactions (<2+ reaction grade), extra serum reactions, samples from patients who had received stem cell transplants, ABO subgroups, and specific system error messages. Among the 98 samples showing ≤1+ reaction grade in the AutoVue system, 70 samples (71.4%) showed a normal serum reaction (≥2+ reaction grade) with the manual method, and 28 samples (28.6%) showed weak serum reaction in both methods. Conclusions: ABO blood tying of 97.7% samples could be confirmed by the AutoVue system and a small proportion (2.3%) needed to be re-evaluated by the manual method. Samples with a 2+ reaction grade in serum typing do not need to be evaluated manually, while those with ≤1+ reaction grade do.
Abstract
Background: ABO blood typing in pre-transfusion testing is a major component of the high workload in blood banks that therefore requires automation. We often experienced discrepant results from an automated system, especially weak serum reactions. We evaluated the discrepant results by the reference manual method to confirm ABO blood typing. Methods: In total, 13,113 blood samples were tested with the AutoVue system; all samples were run in parallel with the reference manual method according to the laboratory protocol. Results: The AutoVue system confirmed ABO blood typing of 12,816 samples (97.7%), and these results were concordant with those of the manual method. The remaining 297 samples (2.3%) showed discrepant results in the AutoVue system and were confirmed by the manual method. The discrepant results involved weak serum reactions (<2+ reaction grade), extra serum reactions, samples from patients who had received stem cell transplants, ABO subgroups, and specific system error messages. Among the 98 samples showing ≤1+ reaction grade in the AutoVue system, 70 samples (71.4%) showed a normal serum reaction (≥2+ reaction grade) with the manual method, and 28 samples (28.6%) showed weak serum reaction in both methods. Conclusions: ABO blood tying of 97.7% samples could be confirmed by the AutoVue system and a small proportion (2.3%) needed to be re-evaluated by the manual method. Samples with a 2+ reaction grade in serum typing do not need to be evaluated manually, while those with ≤1+ reaction grade do.
참고문헌 (18)
- Davies A, "End-to-end electronic control of the hospital transfusion process to increase the safety of blood transfusion: strengths and weaknesses", Transfusion, 46, pp.352~364 (2006)
- Sandler SG, "A fully automated blood typing system for hospital transfusion services. ABS2000 Study Group", Transfusion, 40, pp.201~207 (2000)
- Chang C, "Evaluation of Erytra® fully automated analyser for routine use in transfusion laboratory", Transfus Med, 24, pp.33~38 (2014)
- Garratty G, "Advances in red blood cell immunology 1960 to 2009", Transfusion, 50, pp.526~535 (2010)
- Butch SH, "Automation in the transfusion service", Immunohematology, 24, pp.86~92 (2008)
- Cheng YW, "An experience of the introduction of a blood bank automation system (Ortho AutoVue Innova) in a regional acute hospital", Transfus Apher Sci, 53, pp.58~63 (2015)
- Bajpai M, "Automation in immunohematology", Asian J Transfus Sci, 6, pp.140~144 (2012)
- AuBuchon JP, "Emily Cooley Memorial Award. Managing change to improve transfusion safety", Transfusion, 44, pp.1377~1383 (2004)
- Dada A, "Automation and Data Processing in Blood Banking Using the Ortho AutoVue Innova System", Transfus Med Hemother, 34, pp.341~346 (2007)
- 신소연, "수혈 전 검사 장비인 AutoVue Innova와 Techno TwinStation의 평가", Annals of Laboratory Medicine, 28(3), pp.214~220 (2008), 대한진단검사의학회
- 안태규, "ABO-RhD 혈액형 검사와 항체 선별검사를 위한 혈액은행 자동화 장비 AutoVue Innova와 QWALYS-3의 평가", 대한수혈학회지, 23(3), pp.204~209 (2012), 대한수혈학회
- Kim SH, "Evaluation of the Automatic Blood Bank Instrument AutoVue Innova for Antibody Screening", Korean J Blood Transfus, 19, pp.140~145 (2008)
- British Committee for Standards in Haemotology, "Guidelines for pre-transfusion compatibility procedures in blood transfusion laboratories", Transfus Med, 23, pp.3~35 (2013)
- Taylor J, "Multi-centre evaluation of pre-transfusional routine tests using 8-column format gel card (DG Gel®)", Transfus Med, pp.90~98 (2011)
- Cooling L, "Technical manual", American Association of Blood Banks, 291~315 (2014)
- 조덕, "ABO 유전형 검사에 의한 ABO 불일치의 해결", Annals of Laboratory Medicine, 26(2), pp.107~113 (2006), 대한진단검사의학회
- Bhagwat SN, "Comparison between conventional and automated techniques for blood grouping and crossmatching: experience from a tertiary care centre", J Lab Physicians, 7, pp.96~102 (2015)
- Storry JR, "The ABO blood group system revisited: a review and update", Immunohematology, 25, pp.48~59 (2009)