Effects of exogenous hormones treatment on spermiation and plasma levels of gonadal steroids in Roughscale sole, Clidoderma asperrimum
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Roughscale sole (Clidoderma asperrimum) is only wild caught because basic reproductive research on this species is lacking and gamete production in an artificial setting has not been successful. Exogenous hormone treatment has been used to induce gonadal maturation and final spermiation in wild-caught individuals. In this study, the effects of an exogenous hormone on spermiation in roughscale sole was investigated by implanting different concentrations of a salmon gonadotrophin-releasing hormone analog (sGnRH; 0, 25, 50, and 100 μg/kg body weight) into male fishes. The control group did not produce sperm after 21 days post-implantation, and the duration of spermiation was shorter compared to the other groups. The spermiation period and milt amount differed among the hormone-treated groups according to the hormone concentration used. Milt volumes in the groups treated with 25 and 100 μg/kg sGnRH increased compared to the control group, whereas exogenous hormone treatment had no effect on the movable sperm ratio. The spermatocrit was high at the beginning of spermiation in all groups and then tended to decrease gradually over time except in the experimental group treated with 100 μg/kg sGnRH. Plasma levels of testosterone, 11-ketotestosterone, 17α, 20β-dihydroxy-4-pregnen-3-one were not significantly affected by the sGnRH treatments. Our results demonstrate that it is possible to prolong the spermiation period and increase milt volume by treating male roughscale soles with an exogenous hormone. In addition, the artificial hormone treatment did not affect sperm motility.
Abstract
Roughscale sole (Clidoderma asperrimum) is only wild caught because basic reproductive research on this species is lacking and gamete production in an artificial setting has not been successful. Exogenous hormone treatment has been used to induce gonadal maturation and final spermiation in wild-caught individuals. In this study, the effects of an exogenous hormone on spermiation in roughscale sole was investigated by implanting different concentrations of a salmon gonadotrophin-releasing hormone analog (sGnRH; 0, 25, 50, and 100 μg/kg body weight) into male fishes. The control group did not produce sperm after 21 days post-implantation, and the duration of spermiation was shorter compared to the other groups. The spermiation period and milt amount differed among the hormone-treated groups according to the hormone concentration used. Milt volumes in the groups treated with 25 and 100 μg/kg sGnRH increased compared to the control group, whereas exogenous hormone treatment had no effect on the movable sperm ratio. The spermatocrit was high at the beginning of spermiation in all groups and then tended to decrease gradually over time except in the experimental group treated with 100 μg/kg sGnRH. Plasma levels of testosterone, 11-ketotestosterone, 17α, 20β-dihydroxy-4-pregnen-3-one were not significantly affected by the sGnRH treatments. Our results demonstrate that it is possible to prolong the spermiation period and increase milt volume by treating male roughscale soles with an exogenous hormone. In addition, the artificial hormone treatment did not affect sperm motility.
참고문헌 (26)
- Canario AVM, "Levels of 17α, 20α-dihydroxy-4-pregnen-3-one, 3β, 17α, 20α-trihydroxy-5β-pregnane, and other sex steroids, in blood plasma of male dab, Limanda limanda (marine flatfish) injected with human chorionic gonadotrophin", Gen Comp Endocrinol, 83, pp.258~264 (1991)
- Carolsfeld J, "Induced sexual maturation of herring using GnRH ‘quick-release’cholesterol pellets", Aquaculture, 70, pp.169~181 (1988)
- Clearwater SJ, "Gonadotropin releasing hormone-analogue treatment increases sperm motility, seminal plasma pH and sperm production in yellowtail flounder Pleuronectes ferrugineus", Fish Physiol Biochem, 19, pp.349~357 (1998)
- Harmin SA, "Influence of gonadotropic hormone-releasing hormone analog(GnRH-A)on plasma sex steroid profiles and milt production in male winter flounder, Pseudopleuronectes americanus(Walbaum)", Fish Physiol Biochem, 10, pp.399~407 (1993)
- Hunter GA, "5 Hormonal sex control, and its application to fish culture", Fish Physiol, 9, pp.223~303 (1983)
- Júnior JJB, "Gonadorelin increases semen production and does not affect its quality in Leporinus obtusidens", Anim Reprod Sci, 185, pp.154~160 (2017)
- Lim HK, "Effect of exogenous hormones on ovulation and gonadal steroid plasma levels in starry flounder, Platichthys stellatus", Aquacult Int, 24, pp.1061~1071 (2016)
- 임한규, "실내 사육한 강도다리 Platichthys stellatus의 성성숙과 생식주기", 한국양식학회지, 20(4), pp.212~218 (2007), 한국양식학회
- Lim HK, "Effects of gonadotropin releasing hormone analog on milt production enhancement in starry flounder Platichthys stellatus", Fish Sci, 68, pp.1197~1204 (2002)
- 임한규, "실내 사육한 줄가자미(Clidoderma asperrimum)의 성 성숙과 생식주기", 수산해양교육연구, 24(6), pp.1003~1012 (2012), 한국수산해양교육학회
- Lim HK, "Effects of slow release gonadotropin releasing hormone analog on milt characteristics and plasma levels of gonadal steroids in greenback flounder, Rhombosolea tapirina", Aquaculture, 240, pp.505~516 (2004)
- 임한규, "Luteinising Hormone Releasing Hormone Analogue과 Human Chorionic Gonadotropin 처리가 박대(Cynoglossus semilaevis)의 배정에 미치는 영향", 수산해양교육연구, 28(2), pp.596~602 (2016), 한국수산해양교육학회
- Mañanós E, "Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin-releasing hormone analogue", J Fish Biol, 60, pp.328~339 (2002)
- Moon SH, "Increased plasma 17-hydroxyprogesterone and milt production in response to gonadotropin-releasing hormone agonist in captive male starry flounder, Platichthys stellatus", Aquaculture, 218, pp.703~716 (2003)
- Mylonas CC, "Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone", Aquaculture, 202, pp.205~220 (2001)
- Mylonas CC, "Use of GnRHa-delivery systems for the control of reproduction in fish", Rev Fish Biol Fish, 10, pp.463~491 (2000)
- Mylonas CC, "Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality", Aquaculture, 472, pp.21~44 (2017)
- Mylonas CC, "Broodstock management and hormonal manipulations of fish reproduction", Gen Comp Endocrinol, 165, pp.516~534 (2010)
- Aquacultural technique development of roughscale sole, "National Fisheries Research and Development Institute (NFRDI)", NFRDI, 61~ (2011)
- Pankhurst NW, "Effects of gonadotropin releasing hormone analogue, human chorionic gonadotropin and gonadal steroids on milt volume in the New Zealand snapper, Pagrus auratus(Sparidae)", Aquaculture, 125, pp.185~197 (1994)
- Pankhurst NW, "Milt characteristics and plasma levels of gonadal steroids in greenback flounder Rhombosolea tapirina following treatment with exogenous hormones", Mar Freshwat Behav Physiol, 33, pp.141~159 (2000)
- Pérez L, "Induction of maturation and spermiation in the male European eel : assessment of sperm quality throughout treatment", J Fish Biol, 57, pp.1488~1504 (2000)
- Shangguan B, "Seasonal variations in sperm production and sperm quality in male winter flounder, Pleuronectes americanus : the effects of hypophysectomy, pituitary replacement therapy, and GnRH-a treatment", Mar Biol, 134, pp.19~27 (1999)
- Tokranov AM, "On the distribution and biology of roughscale sole Clidoderma asperrimum(Temminck et Schlegel, 1846)in the Pacific waters off the northern Kuril islands and southeastern Kamchatka", Bull Sea Fish Inst, 159, pp.67~80 (2003)
- Wang N, "Photothermal control of the reproductive cycle in temperate fishes", Rev Aquacult, 2, pp.209~222 (2010)
- Zohar Y, "Endocrine manipulations of spawning in cultured fish: from hormones to genes", Reprod Biotechnol Finfish Aquacult., pp.99~136 (2001)