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旱蓮草 추출물의 항염증 효과

Anti-inflammatory effects of the water extract of Ecliptae Herba

김상찬|지선영|정호준|조희창
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2010.08|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제23권 제2호|pp. 125-138

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참고문헌

키워드

NOIL-6TNF-αPGE2iNOSCOX-2IL-1βEcliptae HerbaNFκBAnti-inflammaory effects
주제분류한의학

초록

Objective : The present study was examined to evaluate the effects of Ecliptae Herba on the production of inflammatory mediators in vivo and in vitro. Methods : In cell viability, all three doses of Ecliptae Herba extract (25, 50 and 100 μg/㎖) had no significant cytotoxicity during the experimental period. The increases of NO production and iNOS expression were detected in LPS-activated cells compared to control, but these increases were dose-dependently attenuated by pre-treatment with Ecliptae Herba extract. Results : 1. LPS plays a pivotal roles in inducing to the massive production of pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 in macrophages. 2. Ecliptae Herba extract reduced the elevated production of cytokines by LPS. 3. Ecliptae Herba extract reduced PGE2 levels in a dose-dependent manner as a consequence of inhibition of COX-2 activity. 4. Ecliptae Herba extract significantly reduced the nuclear translocation of NF-κB induced by LPS. 5. In histopathological study, Ecliptae Herba effectively inhibited the increases of hind paw edema, skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. Conclusions : These results provide evidences that therapeutic effect of Ecliptae Herba on the acute inflammation is partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2through the suppression of NF-κB.

Abstract

Objective : The present study was examined to evaluate the effects of Ecliptae Herba on the production of inflammatory mediators in vivo and in vitro. Methods : In cell viability, all three doses of Ecliptae Herba extract (25, 50 and 100 μg/㎖) had no significant cytotoxicity during the experimental period. The increases of NO production and iNOS expression were detected in LPS-activated cells compared to control, but these increases were dose-dependently attenuated by pre-treatment with Ecliptae Herba extract. Results : 1. LPS plays a pivotal roles in inducing to the massive production of pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 in macrophages. 2. Ecliptae Herba extract reduced the elevated production of cytokines by LPS. 3. Ecliptae Herba extract reduced PGE2 levels in a dose-dependent manner as a consequence of inhibition of COX-2 activity. 4. Ecliptae Herba extract significantly reduced the nuclear translocation of NF-κB induced by LPS. 5. In histopathological study, Ecliptae Herba effectively inhibited the increases of hind paw edema, skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. Conclusions : These results provide evidences that therapeutic effect of Ecliptae Herba on the acute inflammation is partly due to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2through the suppression of NF-κB.

참고문헌 (42)

  1. 정보섭, "도해향약대사전", pp.1043~1044 (2003), 영림사
  2. 본초학교수, "본초학", pp.599~600 (1992), 영림사
  3. 장정애, "한련초 추출물의 모발염색성에 관한 연구", 한국두피모발미용학회지, 2(1), pp.97~109 (2006)
  4. 문영희, "한련초로부터 정제한 다당체의 혈당강하 효과", 생약학회지, 34(3), pp.246~249 (2003), 한국생약학회
  5. 윤정식, "식물자원으로부터 Angiotensin converting enzyme 저해활성 탐색", 한국약용작물학회지, 11(3), pp.246~251 (2003), 한국약용작물학회
  6. 천상욱, "수종의 국화과 식물의 지상부 추출물로부터 살충 및 항균활성 연구", 한국잡초학회지, 23(2), pp.81~91 (2003), 한국잡초학회
  7. 정영돈, "足三里에 施術한 旱蓮草 藥鍼의 免疫增進과 抗癌作用에 관한 硏究", 대한침구학회지, 20(3), pp.13~ (2003), 대한침구학회
  8. 이충열, "흰쥐 신경세포에서 억제성 및 흥분성 한약재가 유발한 이온전류의 특성", 대한한의학회지, 19(2), pp.450~467 (1998)
  9. Lee YS, "IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans", J Microbiol Biotechnol, 10, pp.8~15 (2000)
  10. Higuchi M, "Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages", J Immunol, 144, pp.1425~1431 (1990)
  11. McDaniel ML, "Cytokines and nitric oxides in islet inflammation and diabetes", Proc. Soc. Exp. Biol. Med, 211, pp.24~32 (1996)
  12. Corbett JA, "Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses", J. Exp. Med., 181, pp.559~568 (1995)
  13. Cetkovic-Cvrlje M, "TNF and IFNγ potentiate the deleterious effects of IL-1β on mouse pancreatic islets mainly via generation of nitric oxide", Cytokine, 6, pp.399~406 (1994)
  14. Dohi T, "Hapten-induced colitis is associated with colonic patch hypertrophy and T helper cell 2-type responses", J Exp Med, 189, pp.1169~1180 (1999)
  15. Han. Y, "Preliminary studies on chemocal constiuents and phamacological action of Eclipta prosrata L", Materia Media, 23, pp.680~682 (1983)
  16. Melo PA, "Inhibition of the mytotoxic and hemorrhagic activities of crotalid venoms by Eclipta prostrata (Asteraceae) extracts and constiuents", Toxicon, 32, pp.595~603 (1994)
  17. 국윤범, "황련해독탕이 자발적 고혈압 백서의 혈압 및 신장 기능에 미치는 영향", 대한한의방제학회지, 10(1), pp.113~129 (2002)
  18. Lee JH, "The anti-inflammatory effects of 2 Hz electroacupuncture with different intensities on acute carrageenan-induced inflammation in the rat paw", Int J Mol Med, 16, pp.99~102 (2005)
  19. Gupta M, "Antiinflammatory evaluation of leaves of Plumeria acuminata", BMC Complement Altern Med, 6, pp.36~ (2006)
  20. Rao CV, "Anti-inflammatory and anti-nociceptive activities of Fumaria indica whole plant extract in experimental animals", Acta Pharm, 57(4), pp.491~498 (2007)
  21. Holt S, "Inhibitors of fatty acid amide hydrolase reduce carrageenan-induced hind paw inflammation in pentobarbital-treated mice: comparison with indomethacin and possible involvement of cannabinoid receptors", Br J Pharmacol, 146, pp.467~476 (2005)
  22. Liu J, "Anti-inflammatory effect and mechanism of osthole in rats", Zhong Yao Cai, 28, pp.1002~1006 (2005)
  23. Beloeil H, "Effects of bupivacaine and tetrodotoxin on carrageenan-induced hind paw inflammation in rats (Part 1): hyperalgesia, edema, and systemic cytokines", Anesthesiology, 105, pp.128~138 (2006)
  24. Kim YW, "Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB-dependent iNOS and proinflammatory cytokines production", Br J Pharmacol, 154(1), pp.165~173 (2008)
  25. Cho MK, "JunB", AP-1 and NF-κB-mediated induction of nitric oxide synthase by bovine type I collagen in serum stimulated murine macrophages, 2002, pp.6, 319~332
  26. KIM, HYEONG-DONG, "Effect of Exopolymers from Aureobasidium pullulans on Formalin-Induced Chronic Paw Inflammation in Mice", Journal of Microbiology and Biotechnology, 16(12), pp.1954~1960 (2006), 한국미생물·생명공학회
  27. Charles A Janeway, "Mark Walport, Mark Shlomchik. Immunobiology. 5th", pp.70~71 (2001), Garland Publishing
  28. Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37(6), pp.355~361 (2003)
  29. Jirik FR, "Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells", J Immunol., 142(1), pp.144~147 (1989)
  30. Chen F, "New insights into the role of nuclear factor-kappaB in cell growth regulation", Am J Pathol, 159(2), pp.387~397 (2001)
  31. 손갑호, "시호(柴胡), 인진(茵蔯)의 간암 세포에 대한 항암활성 및 항암제와의 상승(相乘)작용", 대한한의학회지, 16(2), pp.414~432 (1995)
  32. Yoon SB, "Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages", J Ethnopharmacol, 125(2), pp.286~290 (2009)
  33. Van Q, "Antiinflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay", J Ethnopharmacol, 125(3), pp.487~493 (2009)
  34. 노민수, "Inhibitory activities of natural products on lipopolysaccharide induced prostaglandin production in mouse macrophages", 약학회지, 42(6), pp.558~566 (1998)
  35. 서영준, "발암과정에 있어서 cyclooxigenase-2의 역할 및 그 저해를 통한 화학 암예방", 분자세포생물학뉴스, 13, pp.8~17 (2001)
  36. Nathan C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6, pp.3051~3064 (1992)
  37. Wang T, "Effects of escin on acute inflammation and the immune system in mice", Pharmacol Rep, 61(4), pp.697~704 (2009)
  38. Tag H, "Evaluation of anti-inflammatory potential of Chloranthus erectus (Buch.-Ham.) Verd. leaf extract in rats", J Ethnopharmacol, 126(2), pp.371~374 (2009)
  39. Gurgel AP, "Oliveira RA, Souza IA. In vivo study of the anti-inflammatory and antitumor activities of leaves from Plectranthus amboinicus (Lour.) Spreng (Lamiaceae)", J Ethnopharmacol, 125(2), pp.361~363 (2009)
  40. Wu Y, "Effect of total phenolics from Laggera alata on acute and chronic inflammation models", J Ethnopharmacol, 108(2), pp.243~250 (2006)
  41. Wise LE, "Evaluation of fatty acid amides in the carrageenaninduced paw edema model", Neuropharmacology, 54(1), pp.181~188 (2008)
  42. Babu NP, "Anti-inflammatory activity of Albizia lebbeck Benth., an ethnomedicinal plant, in acute and chronic animal models of inflammation", J Ethnopharmacol, 125(2), pp.356~360 (2009)

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