가토 모델에서 소양막콘택트렌즈의 창상 치유 효과
The Wound Healing Effects of AmniSite-Lens in Rabbits
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적: 가토의 각막 화학 화상 및 각막 절제술 시행 후 AmniSite-Lens(소양막 콘택트렌즈)의 창상 치유 효과를 평가하였다. 대상과 방법: 치료용 콘택트렌즈(FocusⓇ 1-2 week)를 착용시킨 것을 대조군으로 하였으며, 실험군은 AmniSite-Lens를 착용시켰다. 임상 양상 평가는 수술 후 각막 상피 치유 시간 및 각막 혼탁도를 평가하였으며, 조직학적 검사를 통하여 각막 내 염증세포 수 및자멸사 세포 수를 측정하였다. 결과: AmniSite-Lens군은 대조군에 비해 창상 치유 시간은 유의한 차이를 보이지 않았다. 하지만 화학 화상에서는 4주 이후, 각막표면 절제술에서는 1주 이후 유의한 각막 혼탁 호전을 보였다. H&E stain에서 화학 화상 및 표면절제술군 모두 각막 실질 내의 염증세포 수가 치료용 콘택트렌즈에 비해 유의하게 감소하였으며, TUNEL stain에서 화학 화상 및 표면절제술 군에서 각막 세포 자멸사 수가치료용 콘택트렌즈군에 비해 감소하였다. 결론: AmniSite-Lens는 양막의 장점인 각막 창상 치유 혼탁 억제 및 항염증 효과를 보이며, 치료용 콘택트렌즈로서의 장점인 사용과보관의 편리함을 가지고 있다.
Abstract
Purpose: The effects of AmniSite-Lens on wound healing were evaluated for a burn wound on a rabbit cornea. Methods: A chemical burn was inflicted on the cornea of rabbits using 0.1N NaOH and a superficial keratectomy with trephine was performed. The control group consisted of rabbits with a bandage contact lens (Focus Lens) after the operation. In the other group, the AmniSite-Le was applied on the rabbits’ cornea. The rabbits were evaluated for the following: 1) the time of epithelialization; 2) the grade of corneal opacity; and 3) the histological analysis by evaluation of inflammatory reaction and apoptotic keratocytes. Results: In the alkali-burn model, the time of epithelialization in the AmniSite-Lens group was not statistically significant compared with the bandage contact lens group. There was no difference of corneal opacity at postoperative week 1. The corneal opacity in the AmniSite-Lens group was clearer than the bandage contact lens group at postoperative weeks 4 and 8 and the difference of corneal opacity was statistically significant. In the keratectomy model, the time of epithelialization in the AmniSite-Lens group was not statistically significant compared with the bandage contact lens group. The corneal opacity in the AmniSite-Lens group was clearer than the bandage contact lens group at postoperative weeks 1 and 4 and the difference of corneal opacity was statistically significant. Conclusions: The AmniSite-Lens has both the wound healing effect of an amniotic membrane and the convenience of a bandage contact lens.
참고문헌 (12)
- Shimmura S, "Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders", Cornea, 20, pp.408~413 (2001)
- Kim JS, "Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn", Exp Eye Res, 70, pp.329~337 (2000)
- Gris O, "Amniotic membrane implantation as a therapeutic contact lens for the treatment of epithelial disorders", Cornea, 21, pp.22~27 (2002)
- Takano Y, "Dramatic healing of an allergic corneal ulcer persistent for 6 months by amniotic membrane patching in a patient with atopic keratoconjunctivitis: a case report", Cornea, 23, pp.723~725 (2004)
- Choi YS, "Effect of the application of human amniotic membrane on rabbit corneal wound healing after excimer laser photorefractive keratectomy", Cornea, 17, pp.389~395 (1998)
- Woo HM, "Effects of amniotic membrane on epithelial wound healing and stromal remodelling after excimer laser keratectomy in rabbit cornea", Br J Ophthalmol, 85, pp.345~349 (2001)
- 서장원, "양막 콘택트렌즈의 각막 창상치유에 대한 효과", 대한안과학회지, 50(7), pp.989~995 (2009), 대한안과학회
- De Rotth A., "Plastic repair of conjunctival defects with fetal membranes", Arch Ophthalmol, 23, pp.525~525 (1940)
- Batlle JF, "Placental membranes as a conjunctival substitute", Ophthalmology, 100, pp.107~ (1993)
- Kim JC, "Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas", Cornea, 14, pp.473~484 (1995)
- Nakamura T, "Novel clinical application of sterilized, freeze-dried amniotic membrane to treat patients with pterygium", Acta Ophthalmol Scand, 84, pp.401~405 (2006)
- Park M, "Healing of a porcine burn wound dressed with human and bovine amniotic membranes", Wound Repair Regen, 16, pp.520~528 (2008)