추출조건에 따른 콩잎 추출물의 이소플라본 함량 및 이화학적 특성
Changes in Isoflavone Contents and Physicochemical Properties of Soybean Leaf Extract by Extraction Conditions
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초록
To utilize soybean leaf, which is a waste product in soybean production, as a value-added food material, this study aimed to investgate the extraction efficiency of isoflavone from soybean leaf and to characterize physicochemical properties of the extract. Maximum isoflavone content of 59.74 ± 4.54 mg/l was obtained from soybean leaf extracted at 90℃ for 12 h. DPPH (1,1-diphenyl-2-pricylhydrazyl) radical-scavenging activity and total polyphenol contents reached maximum levels of 67.26 ± 3.64% and 1,688.68 ± 97.37 μg/ml chlorogenic acid equivalent, respectively. Based on the contents of isoflavone and total polyphenol, and DPPH radical-scavenging activity, optimum extraction conditions for soybean leaf using water as solvent were 90℃ and 12 h.
Abstract
To utilize soybean leaf, which is a waste product in soybean production, as a value-added food material, this study aimed to investgate the extraction efficiency of isoflavone from soybean leaf and to characterize physicochemical properties of the extract. Maximum isoflavone content of 59.74 ± 4.54 mg/l was obtained from soybean leaf extracted at 90℃ for 12 h. DPPH (1,1-diphenyl-2-pricylhydrazyl) radical-scavenging activity and total polyphenol contents reached maximum levels of 67.26 ± 3.64% and 1,688.68 ± 97.37 μg/ml chlorogenic acid equivalent, respectively. Based on the contents of isoflavone and total polyphenol, and DPPH radical-scavenging activity, optimum extraction conditions for soybean leaf using water as solvent were 90℃ and 12 h.
참고문헌 (29)
- Wada K, "isoflavone intake and stomach cancer risk in Japan: from the takayama study", Int. J. Cancer, 137, pp.885~892 (2015)
- Nam H, "Development of bioactive peptides and its market trend", Food Indust. Nutr, 4, pp.17~19 (1999)
- Choi Y, "Dose response relationship of isoflavone supplementation on plasma lipid profiles and total antioxidant status in permenopausal and postmenopausal women", Korean Nutri. Soci, 34, pp.322~329 (2001)
- 이숙희, "콩잎 김치의 숙성에 미치는 소금농도의 효과", Food Science and Preservation, 10(4), pp.512~516 (2003), 한국식품저장유통학회
- 김민경, "콩잎 품종에 따른 이화학적 특성 비교 및 콩잎 분말을 첨가한 두부 개발", 한국식품조리과학회지, 27(5), pp.557~565 (2011), 한국식품조리과학회
- 류승희, "콩잎 및 콩잎 요리의 이소플라본 함량 및 항산화 관련 성분들의 비교", 한국식품조리과학회지, 21(4), pp.433~439 (2005), 한국식품조리과학회
- Ho HM, "Vascular effects of a soy leaves (Glycine max) extract and kaempferol glycosides in isolated rat carotid arteries", Planta. Med, 68, pp.487~491 (2002)
- Li H, "Soy leaf extract containing kaempferol glycosides and pheophorbides improves glucose homeostasis by enhancing pancreatic beta-cell function and suppressing hepatic lipid accumulation in db", db Mice, 2015, pp.63, 7198~7210
- Han JM, "Soy-leaf extract exerts atheroprotective effects via modulation of kruppel-like factor 2 and adhesion molecules", Int. J. Mol. Sci, 18, pp.21~26 (2017)
- 김재원, "해조분말 추출물의 건조방법에 따른 품질 및 항산화 특성", 한국식품과학회지, 44(6), pp.716~721 (2012), 한국식품과학회[DOI]
- 강재란, "반응표면분석을 이용한 흑마늘의 항산화 활성 최적화를 위한 추출조건 확립", Food Science and Preservation, 19(4), pp.577~585 (2012), 한국식품저장유통학회
- 이창용, "폴리페놀 함량과 항산화력에 따른 로즈마리의 최적 추출조건 확립", 한국식품과학회지, 45(4), pp.501~507 (2013), 한국식품과학회[DOI]
- 권유리, "추출방법에 따른 톳 추출물의 항산화 및 생리활성 특성", Food Science and Preservation, 24(5), pp.631~637 (2017), 한국식품저장유통학회
- 정진호, "동백나무(Camellia japonica) 잎의 열수추출 조건 확립", 한국식품과학회지, 38(6), pp.823~828 (2006), 한국식품과학회
- 인만진, "보리잎 분말차의 제조와 그 품질특성", 한국식품영양과학회지, 35(6), pp.734~737 (2006), 한국식품영양과학회
- 권구중, "화살나무 잎 열수추출물의 생리활성", 한국식품과학회지, 39(5), pp.569~574 (2007), 한국식품과학회
- Alam A, "Simple, efficient and economical methods for isolation and estimation of novel isoflavone using RP-HPLC", MethodsX, 4, pp.128~133 (2017)
- 한선경, "미생물발효차(Camellia sinensis L.) 제조과정 중의 품질특성 변화", 한국식품과학회지, 42(1), pp.21~26 (2010), 한국식품과학회
- Folin O, "phophotungstic-phosphomolybdic compounds as color reagents", J. Biol. Chem, 12, pp.239~243 (1912)
- 우관식, "반응표면분석에 의한 유자씨 에탄올 추출물의 항산화활성", 한국식품영양과학회지, 38(3), pp.384~390 (2009), 한국식품영양과학회
- Duncan DB, "Multiple range and multiple F test", Biometrics, 11, pp.1~42 (1955)
- HEO, SUNYEON, "Characterization of an Extracellular Xylanase in Paenibacillus sp. HY-8Isolated from an Herbivorous Longicorn Beetle", Journal of Microbiology and Biotechnology, 16(11), pp.1753~1759 (2006), 한국미생물·생명공학회
- Choi JS, "Isoflavone contents in some varieties of soybean", Food Sci. Biotechnol, 5, pp.167~169 (1996)
- 신지훈, "조리방법을 달리한 쥐눈이콩의 항산화력 및 이소플라본 배당체·비배당체 함량 비교", 한국식품조리과학회지, 32(2), pp.197~203 (2016), 한국식품조리과학회[DOI]
- 이명희, "콩 및 콩 가공식품의 이소플라본 함량", 한국식품과학회지, 34(3), pp.365~369 (2002), 한국식품과학회
- Kim YM, "Changes of enzyme activity, trypsin inhibitor, tannin and phytic acid during heat treatment of soybean", Korean J. Food Sci. Technol, 30, pp.1012~1017 (1998)
- Bressani R, "Tannin in common beans : methods of analysis and effects on protein quality", J. Food Sci, 48, pp.1000~1001 (1983)
- 김종필, "작두콩 추출물의 화학적 특성 및 DPPH 라디컬 소거능", 한국식품과학회지, 44(4), pp.441~446 (2012), 한국식품과학회
- 이란숙, "검은 콩 및 노란 콩의 품질 특성 및 콩 부위별 항산화 활성", 한국식품과학회지, 46(6), pp.757~761 (2014), 한국식품과학회[DOI]